{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:40:07Z","timestamp":1790854807525,"version":"4.1.0"},"reference-count":65,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T00:00:00Z","timestamp":1697673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/N018214\/1"],"award-info":[{"award-number":["BB\/N018214\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec><jats:title>Abstract<\/jats:title><jats:p>Impairments in myofibrillar protein synthesis (MyoPS) during bed rest accelerate skeletal muscle loss in older adults, increasing the risk of adverse secondary health outcomes. We investigated the effect of prior resistance exercise (RE) on MyoPS and muscle morphology during a disuse event in 10 healthy older men (65\u201380\u00a0years). Participants completed a single bout of unilateral leg RE the evening prior to 5\u00a0days of in\u2010patient bed\u2010rest. Quadriceps cross\u2010sectional area (CSA) was determined prior to and following bed\u2010rest. Serial muscle biopsies and dual stable isotope tracers were used to determine rates of integrated MyoPS (iMyoPS) over a 7\u00a0day habitual \u2018free\u2010living\u2019 phase and the bed\u2010rest phase, and rates of acute postabsorptive and postprandial MyoPS (aMyoPS) at the end of bed rest. Quadriceps CSA at 40%, 60% and 80% of muscle length significantly decreased in exercised (EX) and non\u2010exercised control (CTL) legs with bed\u2010rest. The decline in quadriceps CSA at 40% and 60% of muscle length was attenuated in EX compared with CTL. During bed\u2010rest, iMyoPS rates decreased from habitual values in CTL, but not EX, and were significantly different between legs. Postprandial aMyoPS rates increased above postabsorptive values in EX only. The change in iMyoPS over bed\u2010rest correlated with the change in quadriceps CSA in CTL, but not EX. A single bout of RE attenuated the decline in iMyoPS rates and quadriceps atrophy with 5\u00a0days of bed\u2010rest in older men. Further work is required to understand the functional and clinical implications of prior RE in older patient populations.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp15779-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec><jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Age\u2010related skeletal muscle deterioration, linked to numerous adverse health outcomes, is driven by impairments in muscle protein synthesis that are accelerated during periods of disuse.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Resistance exercise can stimulate muscle protein synthesis over several days of recovery and therefore could counteract impairments in this process that occur in the early phase of disuse.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>In the present study, we demonstrate that the decline in myofibrillar protein synthesis and muscle atrophy over 5\u00a0days of bed\u2010rest in older men was attenuated by a single bout of unilateral resistance exercise performed the evening prior to bed\u2010rest.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>These findings suggest that concise resistance exercise intervention holds the potential to support muscle mass retention in older individuals during short\u2010term disuse, with implications for delaying sarcopenia progression in ageing populations.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1113\/jp285130","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T16:39:59Z","timestamp":1697733599000},"page":"87-105","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["A single bout of prior resistance exercise attenuates muscle atrophy and declines in myofibrillar protein synthesis during bed\u2010rest in older men"],"prefix":"10.1113","volume":"603","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0327-5726","authenticated-orcid":false,"given":"Benoit","family":"Smeuninx","sequence":"first","affiliation":[{"name":"School of Sport, Exercise and Rehabilitation Sciences University of Birmingham  Birmingham UK"},{"name":"Cellular &amp; Molecular Metabolism Laboratory Monash University  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasir S.","family":"Elhassan","sequence":"additional","affiliation":[{"name":"Institute of Metabolism and Systems Research University of Birmingham  Birmingham UK"},{"name":"Centre for Endocrinology, Diabetes and Metabolism Birmingham Health Partners  Birmingham 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