{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T06:11:23Z","timestamp":1774332683517,"version":"3.50.1"},"reference-count":109,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T00:00:00Z","timestamp":1707696000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Voltage\u2010gated Na\n                      <jats:sup>+<\/jats:sup>\n                      channels are crucial to action potential propagation in excitable tissues. Because of the high amplitude and rapid activation of the Na\n                      <jats:sup>+<\/jats:sup>\n                      current, voltage\u2010clamp measurements are very challenging and are usually performed at room temperature. In this study, we measured Na\n                      <jats:sup>+<\/jats:sup>\n                      current voltage\u2010dependence in stem cell\u2010derived cardiomyocytes at physiological temperature. While the apparent activation and inactivation curves, measured as the dependence of current amplitude on voltage, fall within the range reported in previous studies, we identified a systematic error in our measurements. This error is caused by the deviation of the membrane potential from the command potential of the amplifier. We demonstrate that it is possible to account for this artifact using computer simulation of the patch\u2010clamp experiment. We obtained surprising results through patch\u2010clamp model optimization: a half\u2010activation of \u221211.5\u00a0mV and a half\u2010inactivation of \u221287\u00a0mV. Although the half\u2010activation deviates from previous research, we demonstrate that this estimate reproduces the conduction velocity dependence on extracellular potassium concentration.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp15921-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>\n                            Voltage\u2010gated Na\n                            <jats:sup>+<\/jats:sup>\n                            currents play a crucial role in excitable tissues including neurons, cardiac and skeletal muscle.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            Measurement of Na\n                            <jats:sup>+<\/jats:sup>\n                            current is challenging because of its high amplitude and rapid kinetics, especially at physiological temperature.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            We have used the patch\u2010clamp technique to measure human Na\n                            <jats:sup>+<\/jats:sup>\n                            current voltage\u2010dependence in human induced pluripotent stem cell\u2010derived cardiomyocytes.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            The patch\u2010clamp data were processed by optimization of the model accounting for voltage\u2010clamp experiment artifacts, revealing a large difference between apparent parameters of Na\n                            <jats:sup>+<\/jats:sup>\n                            current and the results of the optimization.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            We conclude that actual Na\n                            <jats:sup>+<\/jats:sup>\n                            current activation is extremely depolarized in comparison to previous studies.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            The new Na\n                            <jats:sup>+<\/jats:sup>\n                            current model provides a better understanding of action potential propagation; we demonstrate that it explains propagation in hyperkalaemic conditions.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp285162","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T10:29:25Z","timestamp":1707733765000},"page":"633-661","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Human sodium current voltage\u2010dependence at physiological temperature measured by coupling a patch\u2010clamp experiment to a mathematical model"],"prefix":"10.1113","volume":"602","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-5995-1405","authenticated-orcid":false,"given":"Veronika O.","family":"Abrasheva","sequence":"first","affiliation":[{"name":"Sechenov University  Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandaara G.","family":"Kovalenko","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology  Moscow Russia"},{"name":"M.\u00a0F. Vladimirsky Moscow Regional Research Clinical Institute  Moscow Russia"},{"name":"ITMO University  St Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihail","family":"Slotvitsky","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology  Moscow Russia"},{"name":"M.\u00a0F. Vladimirsky Moscow Regional Research Clinical Institute  Moscow Russia"},{"name":"ITMO University  St Petersburg Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serafima \u0410.","family":"Romanova","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology  Moscow Russia"},{"name":"M.\u00a0F. Vladimirsky Moscow Regional Research Clinical Institute  Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleria A.","family":"Aitova","sequence":"additional","affiliation":[{"name":"Moscow Institute of Physics and Technology  Moscow Russia"},{"name":"M.\u00a0F. 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