{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:56:18Z","timestamp":1791237378079,"version":"4.1.0"},"reference-count":70,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T00:00:00Z","timestamp":1724803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T00:00:00Z","timestamp":1724803200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Acute injury of skeletal muscle disrupts myofibres, microvessels and motor innervation. Myofibre regeneration is well characterized, however its relationship with the regeneration of microvessels and motor nerves is undefined. Endothelial cell (EC) ephrin\u2010B2 (\n                      <jats:italic>Efnb2<\/jats:italic>\n                      ) is required for angiogenesis during embryonic development and promotes neurovascular regeneration in the adult. We hypothesized that, following acute injury to skeletal muscle, loss of EC\n                      <jats:italic>Efnb2<\/jats:italic>\n                      would impair microvascular regeneration and the recovery of neuromuscular junction (NMJ) integrity. Mice (aged 3\u20136\u00a0months) were bred for EC\u2010specific conditional knockout (CKO) of\n                      <jats:italic>Efnb2<\/jats:italic>\n                      following tamoxifen injection with non\u2010injected CKO mice as controls (CON). The gluteus maximus, tibialis anterior or extensor digitorum longus muscle was then injured with local injection of BaCl\n                      <jats:sub>2<\/jats:sub>\n                      . Intravascular staining with wheat germ agglutinin revealed diminished capillary area in the gluteus maximus of CKO\n                      <jats:italic>vs<\/jats:italic>\n                      . CON at 5\u00a0days post\u2010injury (dpi); both recovered to uninjured (0\u00a0dpi) level by 10\u00a0dpi. At 0\u00a0dpi, tibialis anterior isometric force of CKO was less than CON. At 10\u00a0dpi, isometric force was reduced by half in both groups. During intermittent contractions (75\u00a0Hz, 330\u00a0ms\u00a0s\n                      <jats:sup>\u20131<\/jats:sup>\n                      , 120\u00a0s), isometric force fell during indirect (sciatic nerve) stimulation whereas force was maintained during direct (electrical field) stimulation of myofibres. Neuromuscular transmission failure correlated with perturbed presynaptic (terminal Schwann cells) and postsynaptic (nicotinic acetylcholine receptors) NMJ morphology in CKO. Resident satellite cell number on extensor digitorum longus myofibres did not differ between groups. Following acute injury of skeletal muscle, loss of\n                      <jats:italic>Efnb2<\/jats:italic>\n                      in ECs delays capillary regeneration and attenuates recovery of NMJ structure and function.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16243-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>\n                            The relationship between microvascular regeneration and motor nerve regeneration following skeletal muscle injury is undefined. Expression of\n                            <jats:italic>Efnb2<\/jats:italic>\n                            in endothelial cells (ECs) is essential to vascular development and promotes neurovascular regeneration in the adult.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            To test the hypothesis that\n                            <jats:italic>EfnB2<\/jats:italic>\n                            in ECs is required for microvascular regeneration and myofibre reinnervation, we induced conditional knockout of\n                            <jats:italic>Efnb2<\/jats:italic>\n                            in ECs of mice. Acute injury was then induced by BaCl\n                            <jats:sub>2<\/jats:sub>\n                            injection into gluteus maximus, tibialis anterior or extensor digitorum longus (EDL) muscle.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            Capillary regeneration was reduced at 5\u00a0days post\u2010injury (dpi) in gluteus maximus of conditional knockout\n                            <jats:italic>vs<\/jats:italic>\n                            . controls; at 10\u00a0dpi, neither differed from uninjured. Nerve stimulation revealed neuromuscular transmission failure in tibialis anterior with perturbed neuromuscular junction structure. Resident satellite cell number on EDL myofibres did not differ between groups.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            Conditional knockout of EC\n                            <jats:italic>Efnb2<\/jats:italic>\n                            delays capillary regeneration and attenuates recovery of neuromuscular junction structure and function.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp285402","type":"journal-article","created":{"date-parts":[[2024,8,28]],"date-time":"2024-08-28T13:35:25Z","timestamp":1724852125000},"page":"4907-4927","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Inducible deletion of endothelial cell\n                    <i>Efnb2<\/i>\n                    delays capillary regeneration and attenuates myofibre reinnervation following myotoxin injury in mice"],"prefix":"10.1113","volume":"602","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9790-705X","authenticated-orcid":false,"given":"Aaron B.","family":"Morton","sequence":"first","affiliation":[{"name":"Department of Kinesiology and Sport Management Texas A&amp;M University  College Station TX USA"},{"name":"Department of Medical Pharmacology and Physiology University of Missouri  Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1860-4585","authenticated-orcid":false,"given":"Nicole L.","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology and Physiology University of Missouri  Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2221-5999","authenticated-orcid":false,"given":"Alexandra R.","family":"Diller","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences University of Missouri  Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9940-8497","authenticated-orcid":false,"given":"Jacob A.","family":"Kendra","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Sport Management Texas A&amp;M University  College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shadi","family":"Golpasandi","sequence":"additional","affiliation":[{"name":"Department of Kinesiology and Sport Management Texas A&amp;M University  College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D D W","family":"Cornelison","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences University of Missouri  Columbia MO USA"},{"name":"Christopher S. Bond Life Sciences Center University of Missouri  Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5667-2154","authenticated-orcid":false,"given":"Steven S.","family":"Segal","sequence":"additional","affiliation":[{"name":"Department of Medical Pharmacology and Physiology University of Missouri  Columbia MO USA"},{"name":"Dalton Cardiovascular Research Center  Columbia MO USA"},{"name":"Department of Biomedical Sciences University of Missouri  Columbia MO USA"},{"name":"Department of Biomedical, Biological, and Chemical Engineering University of Missouri  Columbia MO USA"},{"name":"Department of Nutrition and Exercise Physiology University of Missouri  Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a001875"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.3.295"},{"key":"e_1_2_6_4_1","volume-title":"Molecular Biology of the Cell 4th edition","author":"Alberts B.","year":"2002"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.759"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-021-04865-4"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abg9509"},{"issue":"2","key":"e_1_2_6_8_1","first-page":"89","article-title":"Skeletal muscle fiber types in C57BL6J mice","volume":"21","author":"Augusto V.","year":"2004","journal-title":"Journal of Morphological Sciences"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.068262"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/10739680490517659"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.061903"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a001735"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/life12050691"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.243699"},{"key":"e_1_2_6_15_1","unstructured":"Calcar J. S. V. Oporinus J. &Vesalius A.(ca. 1546). On the Fabric of the Human Body in Seven Books.Joannes Oporinus Basel Switzerland."},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2008.01.021"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03875"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.07.021"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2016.04.005"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0028090"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0416"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8721"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.03.026"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1171704"},{"issue":"3","key":"e_1_2_6_25_1","first-page":"671","article-title":"Involvement of endothelial PECAM\u20101\/CD31 in angiogenesis","volume":"151","author":"DeLisser H. M.","year":"1997","journal-title":"American Journal of Pathology"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-003-0784-3"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2005.01.008"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1305405"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddu476"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP277247"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00061.2019"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP087558"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00095.2020"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23042254"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00426"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-019-02011-1"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.6.1397"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.116.308620"},{"issue":"72","key":"e_1_2_6_39_1","article-title":"Evaluation of muscle function of the extensor digitorum longus muscle ex vivo and tibialis anterior muscle in situ in mice","author":"Hakim C. H.","year":"2013","journal-title":"Journal of Visualized Experiments: JoVE"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0147198"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1984.66"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2023.114431"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP282292"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP270874"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1993.75.1.341"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.160240"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(06)00256-9"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a009159"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH13-12-1034"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123278"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.45863"},{"issue":"3","key":"e_1_2_6_52_1","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1387\/ijdb.1702679","article-title":"Tissue patterning in the developing mouse limb","volume":"34","author":"Martin P.","year":"1990","journal-title":"International Journal of Developmental Biology"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00501.2020"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13395-019-0213-2"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2024.114818"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(99)80008-7"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7576"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08995"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2089"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1986.61.2.660"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1986.250.3.C474"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.08.252"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-1016-5_7"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201502036"},{"issue":"126","key":"e_1_2_6_65_1","article-title":"Semi\u2010automated analysis of mouse skeletal muscle morphology and fiber\u2010type composition","author":"Tyagi S.","year":"2017","journal-title":"Journal of Visualized Experiments: JoVE"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21165623"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09002"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2018.06.018"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-019-0825-x"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.067678"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3934-04.2004"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP285402","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/JP285402","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP285402","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T01:12:12Z","timestamp":1785460332000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/JP285402"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,28]]},"references-count":70,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1113\/JP285402"],"URL":"https:\/\/doi.org\/10.1113\/jp285402","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,28]]},"assertion":[{"value":"2023-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}