{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T13:01:54Z","timestamp":1788267714946,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T00:00:00Z","timestamp":1708473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["19\u2010CE14\u20100011\u201001"],"award-info":[{"award-number":["19\u2010CE14\u20100011\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:sec><jats:title>Abstract<\/jats:title><jats:p>Renin is the key enzyme of the systemic renin\u2013angiotensin\u2013aldosterone system, which plays an essential role in regulating blood pressure and maintaining electrolyte and extracellular volume homeostasis. Renin is mainly produced and secreted by specialized juxtaglomerular (JG) cells in the kidney. In the present study, we report for the first time that the conserved transmembrane receptor neuropilin\u20101 (NRP1) participates in the development of JG cells and plays a key role in renin production. We used the myelin protein zero\u2010Cre (<jats:italic>P0\u2010Cre<\/jats:italic>) to abrogate <jats:italic>Nrp1<\/jats:italic> constitutively in <jats:italic>P0\u2010Cre<\/jats:italic> lineage\u2010labelled cells of the kidney. We found that the <jats:italic>P0\u2010Cre<\/jats:italic> precursor cells differentiate into renin\u2010producing JG cells. We employed a lineage\u2010tracing strategy combined with RNAscope quantification and metabolic studies to reveal a cell\u2010autonomous role for NRP1 in JG cell function. <jats:italic>Nrp1<\/jats:italic>\u2010deficient animals displayed abnormal levels of tissue renin expression and failed to adapt properly to a homeostatic challenge to sodium balance. These findings provide new insights into cell fate decisions and cellular plasticity operating in <jats:italic>P0\u2010Cre<\/jats:italic>\u2013expressing precursors and identify NRP1 as a novel key regulator of JG cell maturation.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp15957-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec><jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Renin is a centrepiece of the renin\u2013angiotensin\u2013aldosterone system and is produced by specialized juxtaglomerular cells (JG) of the kidney.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Neuropilin\u20101 (NRP1) is a conserved membrane\u2010bound receptor that regulates vascular and neuronal development, cancer aggressiveness and fibrosis progression.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We used conditional mutagenesis and lineage tracing to show that NRP1 is expressed in JG cells where it regulates their function.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Cell\u2010specific <jats:italic>Nrp1<\/jats:italic> knockout mice present with renin paucity in JG cells and struggle to adapt to a homeostatic challenge to sodium balance.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The results support the versatility of renin\u2010producing cells in the kidney and may open new avenues for therapeutic approaches.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1113\/jp285422","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T14:36:04Z","timestamp":1708526164000},"page":"1815-1833","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Neuropilin\u20101 regulates renin synthesis in juxtaglomerular cells"],"prefix":"10.1113","volume":"602","author":[{"given":"Yunzhu","family":"Shen","sequence":"first","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenza","family":"Lotenberg","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Zaworski","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5937-6756","authenticated-orcid":false,"given":"Katharina A.\u2010E.","family":"Broeker","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologie Universit\u00e4t Regensburg  Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Vasseur","sequence":"additional","affiliation":[{"name":"Institut Necker Enfants Malades, Growth and Signalling departement Universit\u00e9 Paris Cit\u00e9, INSERM U1151, CNRS UMR 8253  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7008-9352","authenticated-orcid":false,"given":"Liliane","family":"Louedec","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7075-4655","authenticated-orcid":false,"given":"Sandrine","family":"Placier","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Perrine","family":"Fr\u00e8re","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Christine","family":"Verpont","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2771-1596","authenticated-orcid":false,"given":"Pierre","family":"Galichon","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Buob","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8260-6265","authenticated-orcid":false,"given":"Juliette","family":"Hadchouel","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiola","family":"Terzi","sequence":"additional","affiliation":[{"name":"Institut Necker Enfants Malades, Growth and Signalling departement Universit\u00e9 Paris Cit\u00e9, INSERM U1151, CNRS UMR 8253  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos","family":"Chatziantoniou","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5479-6153","authenticated-orcid":false,"given":"Am\u00e9lie","family":"Calmont","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9, INSERM, Unit\u00e9 mixte de Recherche 1155, Kidney Research Centre, H\u00f4pital Tenon  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.14797\/mdcj-14-4-305"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI57301"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00311.2017"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP282615"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-022-02694-8"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/335161a0"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2021.12.028"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd2985"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI41203"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1529"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23034"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/nep.12363"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v21.i23.7065"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/kisup.2014.6"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2017.186"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP270818"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00169-2"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80534-6"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.070112"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2008.10.007"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2022.04.026"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.21.4895"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2017.0240"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80392-X"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2014.08.008"},{"issue":"1","key":"e_1_2_5_28_1","article-title":"ZEB2 controls kidney stromal progenitor differentiation and inhibits abnormal myofibroblast expansion and kidney fibrosis","volume":"8","author":"Kumar S.","year":"2023","journal-title":"Journal of Clinical Investigation Insight"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2941-1"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2012080791"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(74)90291-7"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00030.2016"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00617.2012"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2012.06.026"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0137876"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2021.103209"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2014.11.003"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2018.10.017"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/phy2.132"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0138620"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2006.10.018"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.04.024"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811521106"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.4161\/cam.4.1.10207"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(04)00134-0"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.318064"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81402-6"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/5007"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.22939"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2016.10.010"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2022.05.010"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9323"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd873"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP285422","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,20]],"date-time":"2024-04-20T13:04:17Z","timestamp":1713618257000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/JP285422"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,21]]},"references-count":52,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1113\/JP285422"],"URL":"https:\/\/doi.org\/10.1113\/jp285422","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,21]]},"assertion":[{"value":"2023-11-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}