{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:32:46Z","timestamp":1790742766304,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T00:00:00Z","timestamp":1737936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Fa\u00f1anas cells (FCs) are cerebellar glia of unknown function. First described more than a century ago, they have been almost absent from the scientific literature ever since. Here, we combined whole\u2010cell, patch clamp recordings, near\u2010UV laser photolysis, dye\u2010loading and confocal imaging for a first characterization of FCs in terms of their morphology, electrophysiology and glutamate\u2010evoked currents. We identified FCs of the molecular layer in cerebellar slices by their stubby process and small cell bodies. Despite their more compact shape compared to Bergmann glia (BGs), FCs showed similar membrane resistances and basal currents, suggesting that these passive currents are partly a result of electrical coupling between neighbouring glia. Dye filling and pharmacological experiments confirmed both homo\u2010 and heterotypic gap\u2010junction coupling among FCs and BGs. Parallel\u2010fibre stimulation evoked TTX\u2010sensitive slow inward currents in FCs that were partially blocked by NBQX but not APV. Occasionally, we observed superimposed fast (milliseconds) current transients. Near\u2010UV flash photolysis of MNI\u2010caged glutamate revealed rapid desensitization of these AMPA\u2010receptor mediated currents, which fully recovered only for stimulation intervals &gt;500\u00a0ms. We mapped the highest current densities in proximal processes. We conclude that FCs respond with fast AMPA currents to local glutamate release and they integrate ambient glutamate rises to a slow inward current. Interestingly, we found FCs to prevail throughout adulthood at stable but different densities among cerebellar lobules, with the highest cell densities in lobules I\u2013II and X. Our results strongly suggest that FCs are not just displaced BGs, and that they may have lobule\u2010specific functions \u2010 both locally and at the circuit level, yet to be uncovered.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16459-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Using whole\u2010cell recordings and near\u2010UV laser photolyisis of caged glutamate, we provide a first characterization of cells of Fa\u00f1anas (FCs) in mouse cerebellar slices.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>FCs are present from postnatal day 5 onward throughout adulthood and have a lobule\u2010 dependent density.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Parallel\u2010fibre stimulation generates biphasic, predominantly AMPA\u2010mediated currents in FCs.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Currents induced in FCs by parallel fibre stimulation are not NMDA receptor\u2010dependent and are enhanced upon glutamate\u2010transporter block with TBOA.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Local near\u2010UV glutamate uncaging indicates that FCs can detect fast glutamatergic inputs on the millisecond\u2010time scale.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>FCs functionally integrate into the glial syncytium.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp285949","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T09:53:36Z","timestamp":1737971616000},"page":"855-871","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A first morphological and electrophysiological characterization of Fa\u00f1anas cells of the mouse cerebellum"],"prefix":"10.1113","volume":"603","author":[{"given":"A.","family":"Singer","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, CNRS Saints\u2010P\u00e8res Paris Institute for the Neurosciences  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6704-7303","authenticated-orcid":false,"given":"F.","family":"Trigo","sequence":"additional","affiliation":[{"name":"Departamento de Neurofisiologia Celular y Molecular Instituto de Investigaciones Biol\u00f3gicas Clemente Estable (IIBCE)  Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Vinel","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9, CNRS Saints\u2010P\u00e8res Paris Institute for the Neurosciences  Paris 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