{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T02:18:38Z","timestamp":1777342718250,"version":"3.51.4"},"reference-count":58,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Purkinje neurons are critical for the functioning of the cerebellum, which is among the oldest and most conserved regions of the vertebrate brain. In mammals and in larval zebrafish, Purkinje neurons can generate tonic firing even when isolated from the network. Here we investigated the ionic basis of tonic firing in Purkinje neurons of larval zebrafish using voltage clamp for isolation of membrane currents along with pharmacology. We discovered that these neurons express L\u2010type and P\/Q\u2010type high voltage\u2010gated calcium currents, T\u2010type low voltage\u2010gated calcium currents and SK and BK\u2010type calcium\u2010dependent potassium currents. Among these, L\u2010type calcium currents and SK\u2010type calcium\u2010dependent potassium currents were indispensable for tonic firing, while blocking T\u2010type, P\/Q\u2010type and BK currents had little effect in comparison. We observed that action potentials were broadened when either L\u2010type or SK channels were blocked. Based on these results, we propose that calcium entry via L\u2010type calcium channels activates SK potassium channels leading to faster action potential repolarization, in turn aiding the removal of inactivation of sodium channels. This allows larval zebrafish Purkinje neurons to continue to fire tonically for sustained periods. In mammals also, tonic firing in Purkinje neurons is driven by calcium channels coupling to calcium\u2010dependent potassium channels, yet the specific types of channels involved are different. We therefore suggest that coupling of calcium channels and calcium\u2010dependent potassium channels could be a conserved mechanism for sustaining long bouts of high frequency firing.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp70151-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Tonic firing is an intrinsic property of Purkinje neurons in mammals and fish.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>These neurons express multiple types of voltage\u2010gated conductances including L\u2010type, T\u2010type and P\/Q\u2010type calcium currents and SK\u2010 and BK\u2010type calcium\u2010dependent potassium currents.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Blocking L\u2010type calcium channels and SK\u2010type calcium\u2010dependent potassium channels resulted in spike broadening and reduced tonic firing.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>L\u2010type calcium currents were activated during the repolarization of the spike.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Based on this we conclude that calcium entry via L\u2010type channels activates SK channels causing faster repolarization of the spike and therefore sustained tonic firing.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp286063","type":"journal-article","created":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:25:16Z","timestamp":1760027116000},"page":"7235-7253","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ionic conductances driving tonic firing in Purkinje neurons of larval zebrafish"],"prefix":"10.1113","volume":"603","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8898-1434","authenticated-orcid":false,"given":"Meha P.","family":"Jadhav","sequence":"first","affiliation":[{"name":"National Centre for Biological Sciences Tata Institute of Fundamental Research  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