{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T03:57:15Z","timestamp":1785902235287,"version":"3.56.0"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T00:00:00Z","timestamp":1715731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["MOP\u201093760"],"award-info":[{"award-number":["MOP\u201093760"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102024\u201004520"],"award-info":[{"award-number":["RGPIN\u20102024\u201004520"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:sec><jats:title>Abstract<\/jats:title><jats:p>Skeletal muscle dysfunction is a major problem in critically ill patients suffering from sepsis. This condition is associated with mitochondrial dysfunction and increased autophagy in skeletal muscles. Autophagy is a proteolytic mechanism involved in eliminating dysfunctional cellular components, including mitochondria. The latter process, referred to as mitophagy, is essential for maintaining mitochondrial quality and skeletal muscle health. Recently, a fluorescent reporter system called mito\u2010QC (i.e. mitochondrial quality control) was developed to specifically quantify mitophagy levels. In the present study, we used mito\u2010QC transgenic mice and confocal microscopy to morphologically monitor mitophagy levels during sepsis. To induce sepsis, Mito\u2010QC mice received <jats:italic>Escherichia coli<\/jats:italic> lipopolysaccharide (10\u00a0mg\u00a0kg<jats:sup>\u20131<\/jats:sup> <jats:sc>i.p.<\/jats:sc>) or phosphate\u2010buffered saline and skeletal muscles (hindlimb and diaphragm) were excised 48\u00a0h later. In control groups, there was a negative correlation between the basal mitophagy level and overall muscle mitochondrial content. Sepsis increased general autophagy in both limb muscles and diaphragm but had no effect on mitophagy levels. Sepsis was associated with a downregulation of certain mitophagy receptors (Fundc1, Bcl2L13, Fkbp8 and Phbb2). The present study suggests that general autophagy and mitophagy can be dissociated from one another, and that the characteristic accumulation of damaged mitochondria in skeletal muscles under the condition of sepsis may reflect a failure of adequate compensatory mitophagy.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16103-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec><jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>There was a negative correlation between the basal level of skeletal muscle mitophagy and the mitochondrial content of individual muscles.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Mitophagy levels in limb muscles and the diaphragm were unaffected by lipopolysaccharide (LPS)\u2010induced sepsis.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>With the exception of BNIP3 in sepsis, LPS administration induced either no change or a downregulation of mitophagy receptors in skeletal muscles.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1113\/jp286216","type":"journal-article","created":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T17:59:16Z","timestamp":1715795956000},"page":"2823-2838","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Lack of compensatory mitophagy in skeletal muscles during sepsis"],"prefix":"10.1113","volume":"602","author":[{"given":"Sami","family":"Sedraoui","sequence":"first","affiliation":[{"name":"Meakins\u2010Christie Laboratories, Department of Medicine McGill University  Montreal QC Canada"},{"name":"Translational Research in Respiratory Diseases Program Research Institute of the McGill University 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