{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T16:27:17Z","timestamp":1791995237110,"version":"4.4.0"},"reference-count":78,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["ZIA HL006221"],"award-info":[{"award-number":["ZIA HL006221"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Mitochondria within skeletal muscle cells are located either between the muscle contractile apparatus (interfibrillar mitochondria, IFM) or beneath the cell membrane (subsarcolemmal mitochondria, SSM), with several structural and functional differences reported between IFM and SSM. However, recent 3D imaging studies demonstrate that mitochondria are particularly concentrated in the proximity of capillaries embedded in sarcolemmal grooves rather than in proximity to the sarcolemma itself (paravascular mitochondria, PVM). To evaluate the impact of capillary\n                      <jats:italic>vs<\/jats:italic>\n                      . sarcolemmal proximity, we compared the structure and function of skeletal muscle mitochondria located either lateral to embedded capillaries (PVM), adjacent to the sarcolemma but not in PVM pools (SSM) or interspersed between sarcomeres (IFM). Mitochondrial morphology and interactions were assessed by 3D electron microscopy coupled with machine learning segmentation, whereas mitochondrial energy conversion was assessed by two\u2010photon microscopy of mitochondrial membrane potential, content, calcium, NADH redox and flux in live, intact cells. Structurally, although PVM and SSM were similarly larger than IFM, PVM were larger, rounder and had more physical connections to neighbouring mitochondria compared to both IFM and SSM. Functionally, PVM had similar or greater basal NADH flux compared to SSM and IFM, respectively, despite a more oxidized NADH pool and a greater membrane potential, signifying a greater activation of the electron transport chain in PVM. Together, these data indicate that proximity to capillaries has a greater impact on resting mitochondrial energy conversion and distribution in skeletal muscle than the sarcolemma alone.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16018-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Capillaries have a greater impact on mitochondrial energy conversion in skeletal muscle than the sarcolemma.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Paravascular mitochondria are larger, and the outer mitochondrial membrane is more connected with neighbouring mitochondria.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Interfibrillar mitochondria are longer and have greater contact sites with other organelles (i.e. sarcoplasmic reticulum and lipid droplets).<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Paravascular mitochondria have greater activation of oxidative phosphorylation than interfibrillar mitochondria at rest, although this is not regulated by calcium.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp286246","type":"journal-article","created":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T12:26:11Z","timestamp":1712060771000},"page":"1967-1986","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Impact of capillary and sarcolemmal proximity on mitochondrial structure and energetic function in skeletal muscle"],"prefix":"10.1113","volume":"602","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1182-3623","authenticated-orcid":false,"given":"Hailey A.","family":"Parry","sequence":"first","affiliation":[{"name":"National Lung, Blood, and Heart Institute National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. Bradley","family":"Willingham","sequence":"additional","affiliation":[{"name":"National Lung, Blood, and Heart Institute National Institutes of Health  Bethesda MD USA"},{"name":"Shephard Center's Virginia C. Crawford Research Institute  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin A.","family":"Giordano","sequence":"additional","affiliation":[{"name":"Holy Cross Orthopedic Institute  Fort Lauderdale FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6038-4358","authenticated-orcid":false,"given":"Yuho","family":"Kim","sequence":"additional","affiliation":[{"name":"National Lung, Blood, and Heart Institute National Institutes of Health  Bethesda MD USA"},{"name":"University of Massachusetts  Lowell MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shureed","family":"Qazi","sequence":"additional","affiliation":[{"name":"National Lung, Blood, and Heart Institute National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jay R.","family":"Knutson","sequence":"additional","affiliation":[{"name":"National Lung, Blood, and Heart Institute National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4656-4840","authenticated-orcid":false,"given":"Christian A.","family":"Combs","sequence":"additional","affiliation":[{"name":"National Lung, Blood, and Heart Institute National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8571-244X","authenticated-orcid":false,"given":"Brian","family":"Glancy","sequence":"additional","affiliation":[{"name":"National Lung, Blood, and Heart Institute National Institutes of Health  Bethesda MD USA"},{"name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases National Institutes of Health  Bethesda MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00031.2005"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2012.03613.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2728(78)90104-4"},{"key":"e_1_2_6_5_1","first-page":"175","article-title":"Ontogenesis of mitochondrial reticulum in rat diaphragm muscle","volume":"25","author":"Bakeeva L. 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