{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:27:06Z","timestamp":1789709226671,"version":"4.0.1"},"reference-count":84,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T00:00:00Z","timestamp":1728259200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Sensory hair cells of the inner ear utilize specialized ribbon synapses to transmit sensory stimuli to the central nervous system. This transmission necessitates rapid and sustained neurotransmitter release, which depends on a large pool of synaptic vesicles at the hair\u2010cell presynapse. While previous work in neurons has shown that kinesin motor proteins traffic synaptic material along microtubules to the presynapse, the mechanisms of this process in hair cells remain unclear. Our study demonstrates that the kinesin motor protein Kif1a, along with an intact microtubule network, is essential for enriching synaptic vesicles at the presynapse in hair cells. Through genetic and pharmacological approaches, we disrupt Kif1a function and impair microtubule networks in hair cells of the zebrafish lateral\u2010line system. These manipulations led to a significant reduction in synaptic\u2010vesicle populations at the presynapse in hair cells. Using electron microscopy,\n                      <jats:italic>in vivo<\/jats:italic>\n                      calcium imaging, and electrophysiology, we show that a diminished supply of synaptic vesicles adversely affects ribbon\u2010synapse function.\n                      <jats:italic>Kif1aa<\/jats:italic>\n                      \u00a0mutants exhibit dramatic reductions in spontaneous vesicle release and evoked postsynaptic calcium responses. Furthermore,\n                      <jats:italic>kif1aa<\/jats:italic>\n                      \u00a0mutants exhibit impaired rheotaxis, a behaviour reliant on the ability of hair cells in the lateral line to respond to sustained flow stimuli. Overall, our results demonstrate that Kif1a\u2010mediated microtubule transport is critical to enrich synaptic vesicles at the active zone, a process that is vital for proper ribbon\u2010synapse function in hair cells.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16337-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>\n                            <jats:italic>Kif1a<\/jats:italic>\n                            mRNAs are present in zebrafish hair cells.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Loss of Kif1a disrupts the enrichment of synaptic vesicles at ribbon synapses.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Disruption of microtubules depletes synaptic vesicles at ribbon synapses.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            <jats:italic>Kif1aa<\/jats:italic>\n                            \u00a0mutants have impaired ribbon\u2010synapse and sensory\u2010system function.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp286263","type":"journal-article","created":{"date-parts":[[2024,10,7]],"date-time":"2024-10-07T09:24:20Z","timestamp":1728293060000},"page":"6391-6421","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Kif1a and intact microtubules maintain synaptic\u2010vesicle populations at ribbon synapses in zebrafish hair cells"],"prefix":"10.1113","volume":"603","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4888-0708","authenticated-orcid":false,"given":"Sandeep","family":"David","sequence":"first","affiliation":[{"name":"Section on Sensory Cell Development and Function National Institute on Deafness and other Communication Disorders  Bethesda Maryland USA"},{"name":"National Institutes of Health\u2013Brown University Graduate Partnership Program  Bethesda Maryland 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