{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T06:04:29Z","timestamp":1792476269480,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T00:00:00Z","timestamp":1724630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"\n Abstract<\/jats:title>\n \n Spatially selective vagus nerve stimulation (sVNS) offers a promising approach for addressing heart disease with enhanced precision. Despite its therapeutic potential, VNS is limited by off\u2010target effects and the need for time\u2010consuming titration. Our research aimed to determine the spatial organization of cardiac afferent and efferent fibres within the vagus nerve of pigs to achieve targeted neuromodulation. Using trial\u2010and\u2010error sVNS\n in vivo<\/jats:italic>\n and\n ex vivo<\/jats:italic>\n micro\u2010computed tomography fascicle tracing, we found significant spatial separation between cardiac afferent and cardiac efferent fibres at the mid\u2010cervical level and they were localized on average on opposite sides of the nerve cross\u2010section. This was consistent between both\n in vivo<\/jats:italic>\n and\n ex vivo<\/jats:italic>\n methods. Specifically, cardiac afferent fibres were located near pulmonary fibres, consistent with findings of cardiopulmonary convergent circuits and, notably, cardiac efferent fascicles were exclusive. These cardiac efferent regions were located in close proximity to the recurrent laryngeal regions. This is consistent with the roughly equitable spread across the nerve of the afferent and efferent fibres. Our study demonstrated that targeted neuromodulation via sVNS could achieve scalable heart rate decreases without eliciting cardiac afferent\u2010related reflexes; this is desirable for reducing sympathetic overactivation associated with heart disease. These findings indicate that understanding the spatial organization of cardiac\u2010related fibres within the vagus nerve can lead to more precise and effective VNS therapy, minimizing off\u2010target effects and potentially mitigating the need for titration.\n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>\n \n Key points<\/jats:title>\n \n \n \n Spatially selective vagus nerve stimulation (sVNS) presents a promising approach for addressing chronic heart disease with enhanced precision.<\/jats:p>\n <\/jats:list-item>\n \n Our study reveals significant spatial separation between cardiac afferent and efferent fibres in the vagus nerve, particularly at the mid\u2010cervical level.<\/jats:p>\n <\/jats:list-item>\n \n \n Utilizing trial\u2010and\u2010error sVNS\n in vivo<\/jats:italic>\n and micro\u2010computed tomography fascicle tracing, we demonstrate the potential for targeted neuromodulation, achieving therapeutic effects such as scalable heart rate decrease without stimulating cardiac afferent\u2010related reflexes.\n <\/jats:p>\n <\/jats:list-item>\n \n This spatial understanding opens avenues for more effective VNS therapy, minimizing off\u2010target effects and potentially eliminating the need for titration, thereby expediting therapeutic outcomes in myocardial infarction and related conditions.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1113\/jp286494","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T03:45:25Z","timestamp":1724643925000},"page":"1983-2004","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Towards spatially selective efferent neuromodulation: anatomical and functional organization of cardiac fibres in the porcine cervical vagus 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