{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:21:25Z","timestamp":1787016085620,"version":"build-2736575974"},"reference-count":59,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T00:00:00Z","timestamp":1723593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      The subiculum is a key region of the brain involved in the initiation of pathological activity in temporal lobe epilepsy, and local GABAergic inhibition is essential to prevent subicular\u2010originated epileptiform discharges. Subicular pyramidal cells may be easily distinguished into two classes based on their different firing patterns. Here, we have compared the strength of the GABAa receptor\u2010mediated inhibitory postsynaptic currents received by regular\u2010\n                      <jats:italic>vs<\/jats:italic>\n                      . burst\u2010firing subicular neurons and their dynamic modulation by the activation of \u03bc opioid receptors. We have taken advantage of the sequential re\u2010patching of the same cell to initially classify pyramidal neurons according to their firing patters, and then to measure GABAergic events triggered by the optogenetic stimulation of parvalbumin\u2010 and somatostatin\u2010expressing interneurons. Activation of parvalbumin\u2010expressing cells generated larger responses in postsynaptic burst\u2010firing neurons whereas the opposite was observed for currents evoked by the stimulation of somatostatin\u2010expressing interneurons. In all cases, events depended critically on \u03c9\u2010agatoxin IVA\u2010 but not on \u03c9\u2010conotoxin GVIA\u2010sensitive calcium channels. Optogenetic GABAergic input originating from both parvalbumin\u2010 and somatostatin\u2010expressing cells was reduced in amplitude following the exposure to a \u03bc opioid receptor agonist. The kinetics of this pharmacological sensitivity was different in regular\u2010\n                      <jats:italic>vs<\/jats:italic>\n                      . burst\u2010firing neurons, but only when responses were evoked by the activation of parvalbumin\u2010expressing neurons, whereas no differences were observed when somatostatin\u2010expressing cells were stimulated. In conclusion, our results show that a high degree of complexity regulates the organizing principles of subicular GABAergic inhibition, with the interaction of pre\u2010 and postsynaptic diversity at multiple levels.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16242-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Optogenetic stimulation of parvalbumin\u2010 and somatostatin\u2010expressing interneurons (PVs and SOMs) triggers inhibitory postsynaptic currents (IPSCs) in both regular\u2010 and burst\u2010firing (RFs and BFs) subicular pyramidal cells.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>The amplitude of optogenetically evoked IPSCs from PVs (PV\u2010opto IPSCs) is larger in BFs whereas IPSCs generated by the light activation of SOMs (SOM\u2010opto IPSCs) are larger in RFs.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Both PV\u2010 and SOM\u2010opto IPSCs critically depend on \u03c9\u2010agatoxin IVA\u2010sensitive P\/Q type voltage\u2010gated calcium channels, whereas no major effects are observed following exposure to \u03c9\u2010conotoxin GVIA, suggesting no significant involvement of N\u2010type channels.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            The amplitude of both PV\u2010 and SOM\u2010opto IPSCs is reduced by the probable pharmacological activation of presynaptic \u03bc opioid receptors, with a faster kinetics of the effect observed in PV\u2010opto IPSCs from RFs\n                            <jats:italic>vs<\/jats:italic>\n                            . BFs, but not in SOM\u2010opto IPSCs.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>These results help us understand the complex interactions between different layers of diversity regulating GABAergic input onto subicular microcircuits.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp286679","type":"journal-article","created":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T15:09:43Z","timestamp":1723648183000},"page":"4195-4213","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Multiple layers of diversity govern the cell type specificity of GABAergic input received by mouse subicular pyramidal neurons"],"prefix":"10.1113","volume":"602","author":[{"given":"Nancy Castro","family":"Borjas","sequence":"first","affiliation":[{"name":"Department of Neuroscience, Feinberg School of Medicine Northwestern University  Chicago USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Anst\u00f6tz","sequence":"additional","affiliation":[{"name":"Institute of Anatomy II, Medical Faculty Heinrich\u2010Heine\u2010University  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2147-9236","authenticated-orcid":false,"given":"Gianmaria","family":"Maccaferri","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Feinberg School of Medicine Northwestern University  Chicago USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhab115"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.7b00485"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/mp.2015.113"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(96)13242-0"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.088708"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5034-14.2015"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-018-2848-4"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.031"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-019-0125-5"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1076510"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-21-06676.1996"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.20215"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(96)00251-5"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-020-0278-2"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.1107"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP279561"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19970414)380:3<395::AID-CNE8>3.0.CO;2-Y"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2520-04.2004"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2311885121"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80635-2"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf1836"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69746"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1542"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1255263"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2761-07.2007"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2790"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.21564"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.20931"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0953-816X.2004.03394.x"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.03.034"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1970-11.2011"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1515\/revneuro-2020-0091"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-023-01819-w"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2003.08.002"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2023.09.017"},{"key":"e_1_2_5_37_1","unstructured":"Marino M. Misuri L. &Brogioli D.(2014).A new open source software for the calculation of the liquid junction potential between two solutions according to the stationary Nernst\u2010Planck equation.arXiv.https:\/\/arxiv.org\/abs\/1403.3640v2"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1993.70.3.1244"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.039305"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.10123"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80101-4"},{"key":"e_1_2_5_42_1","volume-title":"Jasper's Basic Mechanisms of the Epilepsies [Internet]","author":"Miles R.","year":"2012"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2009.03.040"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602979103"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050241"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00007.2017"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abg4693"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2022.919773"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.12.013"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.078915"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2000.84.5.2398"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.90566.2008"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00227110"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-10-03544.2000"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2570"},{"issue":"4","key":"e_1_2_5_56_1","first-page":"E2726","article-title":"Frequency\u2010invariant temporal ordering of interneuronal discharges during hippocampal oscillations in awake mice","volume":"109","author":"Varga C.","year":"2012","journal-title":"Proceedings of the National Academy of Sciences of the USA"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.06.004"},{"key":"e_1_2_5_58_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-020-00894-3"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00319"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2023.05.032"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP286679","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,16]],"date-time":"2024-11-16T10:23:19Z","timestamp":1731752599000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/JP286679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,14]]},"references-count":59,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1113\/JP286679"],"URL":"https:\/\/doi.org\/10.1113\/jp286679","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,14]]},"assertion":[{"value":"2024-04-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}