{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T04:21:37Z","timestamp":1783052497488,"version":"3.54.6"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T00:00:00Z","timestamp":1738368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:sec><jats:title>Abstract<\/jats:title><jats:p>Facioscapulohumeral muscular dystrophy (FSHD) is an inherited muscle disease characterized by weakness and muscle wasting. In the absence of available treatments, exercise training has emerged as a potential strategy to attenuate muscle tissue deterioration. However, little is known about the impact of chronic exercise on degenerative events and regenerative capacity in FSHD muscle. Muscle biopsies were obtained from 16 FSHD patients before and after a 24\u00a0week training program combining aerobic\u2010, strength\u2010 and high\u2010intensity exercise (Control; <jats:italic>n<\/jats:italic> = 8, Training; <jats:italic>n<\/jats:italic> = 8). Histochemical and immunohistochemical approaches were applied to assess histopathological signs, markers of regeneration, inflammatory infiltrates and satellite cell content. Muscle telomere length was measured as an indicator of the remaining regenerative capacity. The proportion of muscle fibres expressing developmental myosins and centralized myonuclei was not exacerbated after the intervention. Similarly, no alterations were observed in the number of inflammatory infiltrates (CD68<jats:sup>+<\/jats:sup> cells). Alongside muscle hypertrophy in slow (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.022) and fast fibres (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.022 and <jats:italic>P<\/jats:italic>\u00a0=\u00a00.008), satellite cell content increased specifically in fast fibres (+75 %, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.015), indicating a functional satellite cell pool in FSHD muscle. Importantly, exercise training was not associated with a shortening of muscle telomere length, suggesting that muscle cell turnover was not accelerated despite an expansion of the satellite cell pool. Our findings suggest that combined exercise training elicits beneficial muscular adaptations without impairing important indicators of skeletal muscle regenerative capacity in patients with FSHD.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16543-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec><jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>A 24\u00a0week combined exercise training program is a safe and well\u2010tolerated strategy to attenuate skeletal muscle deterioration in facioscapulohumeral muscular dystrophy (FSHD) patients.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Markers of histopathology, muscle fibre regeneration and inflammatory infiltrates were not exacerbated following exercise training in FSHD muscle.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Here, we show novel data that exercise training in FSHD patients induced muscle fibre hypertrophy and triggered an expansion of the satellite cell pool specifically in fast fibres.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Exercise training in these patients is not associated with a shortening of muscle telomere length thereby indicating a preserved capacity for muscle regeneration.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1113\/jp287033","type":"journal-article","created":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T09:14:06Z","timestamp":1738401246000},"page":"1057-1069","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Increased muscle satellite cell content and preserved telomere length in response to combined exercise training in patients with FSHD"],"prefix":"10.1113","volume":"603","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3500-2896","authenticated-orcid":false,"given":"Oscar","family":"Horwath","sequence":"first","affiliation":[{"name":"School of Health Sciences \u00d6rebro University  \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3268-1544","authenticated-orcid":false,"given":"Diego","family":"Montiel\u2010Rojas","sequence":"additional","affiliation":[{"name":"School of Health Sciences \u00d6rebro University  \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8071-4745","authenticated-orcid":false,"given":"Elodie","family":"Ponsot","sequence":"additional","affiliation":[{"name":"School of Health Sciences \u00d6rebro University  \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8163-5539","authenticated-orcid":false,"given":"L\u00e9onard","family":"F\u00e9asson","sequence":"additional","affiliation":[{"name":"Inter\u2010University Laboratory of Human Movement Sciences University Lyon, UJM\u2010Saint\u2010Etienne  Saint\u2010Etienne France"},{"name":"Myology Unit, Referent Center of Rare Neuromuscular Diseases, Euro\u2010NmD University Hospital of Saint\u2010Etienne  Saint\u2010Etienne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9831-0896","authenticated-orcid":false,"given":"Fawzi","family":"Kadi","sequence":"additional","affiliation":[{"name":"School of Health Sciences \u00d6rebro University  \u00d6rebro Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.21.10114"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-017-8497-9"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000001808"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880181312"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddaa164"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12231"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000004497"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2008.00368.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.15480"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.880090611"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2008.07.022"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60071-3"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.10.e47"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600056"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2016.04.002"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.10394"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.141481"},{"key":"e_1_2_5_19_1","doi-asserted-by":"crossref","unstructured":"Elkin L. A. Kay M. Higgins J. J. &Wobbrock J. O.(2021).An aligned rank transform procedure for multifactor contrast tests. In:UIST '21: The 34th Annual ACM Symposium on User Interface Software and Technology 754\u2013768.","DOI":"10.1145\/3472749.3474784"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-201X.2005.01457.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2020.00958"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00893.2019"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/sms.13848"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmd.2024.01.003"},{"key":"e_1_2_5_25_1","first-page":"1","article-title":"Adaptation of human skeletal muscle to training and anabolic steroids","volume":"646","author":"Kadi F.","year":"2000","journal-title":"Acta Physiologica Scandinavica Supplementum"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-005-0102-0"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0838.2009.00966.x"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1249\/mss.0b013e3181596695"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.065904"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.2883"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.27074"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.067595"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00789.2010"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0838.2010.01178.x"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202101096R"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/function\/zqaa009"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2010.00016"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000150584.45055.27"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0052769"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01215.2007"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2011.063818"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.20964"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-010-1353-6"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2014.05.021"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12609"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.3233\/JND-140034"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000134660.30793.1F"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.21054"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000001008"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11910-016-0667-0"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2008.04.034"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehy585"},{"key":"e_1_2_5_53_1","doi-asserted-by":"crossref","unstructured":"Wobbrock J. O. Findlater L. Gergle D. &Higgins J. J.(2011).The aligned rank transform for nonparametric factorial analyses using only anova procedures. In:CHI '11: Proceedings of the SIGCHI Conference on Human Factors in Computing Systems.143\u2013146.","DOI":"10.1145\/1978942.1978963"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP287033","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T10:08:42Z","timestamp":1754906922000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/JP287033"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2]]},"references-count":52,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1113\/JP287033"],"URL":"https:\/\/doi.org\/10.1113\/jp287033","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2]]},"assertion":[{"value":"2024-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}