{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T23:56:29Z","timestamp":1789170989894,"version":"build-2803163510"},"reference-count":97,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,11]],"date-time":"2025-05-11T00:00:00Z","timestamp":1746921600000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:sec><jats:title>Abstract<\/jats:title><jats:p>Increasing evidence indicates resveratrol (RES) supplementation evokes anti\u2010obesogenic responses that could mitigate obesity\u2010induced reductions in skeletal muscle (SkM) contractility. Contractile function is a key facet of SkM health that underpins whole body health. For the first time, the present study examines the effects of a high\u2010fat diet and RES supplementation on isolated soleus (SOL) and extensor digitorum longus (EDL) contractile function. Female CD\u20101\u00a0mice, \u223c6\u00a0weeks old (<jats:italic>n<\/jats:italic>\u00a0=\u00a038), consumed a standard laboratory diet (SLD) or a high\u2010fat diet (HFD), with or without RES (4\u00a0g\u00a0kg<jats:sup>\u22121<\/jats:sup> diet) for 12\u00a0weeks. SOL and EDL (<jats:italic>n<\/jats:italic>\u00a0=\u00a08\u201310 per muscle, per group) were isolated and then absolute and normalised (to muscle size and body mass) isometric force and work loop power output (PO) were measured, and fatigue resistance was determined. Furthermore, sirtuin\u20101 expression was determined to provide mechanistic insight into any potential contractile changes. For SOL absolute force was higher in HFDRES compared to HFD (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.033), and PO normalised to body mass and cumulative work during fatigue were reduced in HFD groups (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.014). EDL absolute and normalised PO and cumulative work during fatigue were lower in HFD compared to other groups (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.019). RES negated most adverse effects of HFD consumption on EDL contractility, with HFDRES producing PO and cumulative work comparable to the SLD groups. Sirtuin\u20101 expression was not influenced by diet in either muscle (<jats:italic>P\u00a0<\/jats:italic>&gt;\u00a00.165). This study uniquely demonstrates that RES attenuates HFD\u2010induced reductions in contractile performance of EDL, but this response is not explained by altered sirtuin\u20101 expression. These results suggest RES may be an appropriate strategy to alleviate obesity\u2010induced declines in SkM function.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16688-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec><jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Skeletal muscle health, a precursor for disease prevention, whole body health and quality of life, is substantially reduced because of obesity.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Growing evidence suggests that the anti\u2010obesogenic effects of nutritional supplement resveratrol may mitigate against obesity\u2010induced muscle pathology. However, the effect of resveratrol on skeletal muscle contractile performance, a primary marker of skeletal muscle health, is yet to be examined.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Our findings indicate that resveratrol reduces the adverse effects of high\u2010fat diet consumption on the contractile performance of isolated fast twitch muscle and reduces the accumulation of central adipose.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Resveratrol had little effect on skeletal muscle performance of standard diet mice, highlighting its specific efficacy in addressing high\u2010fat diet\u2010induced muscle pathology.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1113\/jp287056","type":"journal-article","created":{"date-parts":[[2025,5,11]],"date-time":"2025-05-11T15:38:21Z","timestamp":1746977901000},"page":"2675-2698","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Adverse effects of high\u2010fat diet consumption on contractile mechanics of isolated mouse skeletal muscle are reduced when supplemented with resveratrol"],"prefix":"10.1113","volume":"603","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9032-1868","authenticated-orcid":false,"given":"Sharn P.","family":"Shelley","sequence":"first","affiliation":[{"name":"Research Centre for Physical Activity, Sport and Exercise Science Coventry University  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob S.","family":"James","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences University of Bradford  Bradford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven J.","family":"Eustace","sequence":"additional","affiliation":[{"name":"Research Centre for Physical Activity, Sport and Exercise Science Coventry University  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5503-6188","authenticated-orcid":false,"given":"Mark C.","family":"Turner","sequence":"additional","affiliation":[{"name":"Research Centre for Health and Life Sciences Coventry University  Coventry UK"},{"name":"Institute for Cardio\u2010Metabolic Medicine University Hospital Coventry and Warwickshire  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan","family":"Brett","sequence":"additional","affiliation":[{"name":"Research Centre for Health and Life Sciences Coventry University  Coventry UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma L. 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