{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T23:07:01Z","timestamp":1779232021303,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T00:00:00Z","timestamp":1740096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007334","name":"Ministry of Health and Family Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007334","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009104","name":"Department of Health Research, India","doi-asserted-by":"publisher","award":["R.11012\/03\/2020\u2010HR"],"award-info":[{"award-number":["R.11012\/03\/2020\u2010HR"]}],"id":[{"id":"10.13039\/501100009104","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:sec><jats:title>Abstract<\/jats:title><jats:p>Sulphur dioxide (SO\u2082) is a toxic air pollutant with deleterious effects on the respiratory, cardiovascular, endocrine and reproductive systems. SO\u2082, being freely diffusible, can cross the placenta and reach the fetal system. Previous studies have reported the association between SO\u2082 exposure and adverse pregnancy and fetal outcomes without much emphasis on its molecular aspects. The present study has investigated the adverse consequences of SO\u2082 derivatives on the two key trophoblast functions critical for placentation: invasion and fusion. SO\u2082 exposure inhibited the fusogenic potential of BeWo cells, as well as the invasion and migratory ability of the extravillous HTR8\/SVneo cells. A molecular insight revealed dysregulated endoplasmic reticulum stress, with an altered epigenetic state as seen by histone modifications following SO\u2082 exposure. Collectively, our findings revealed the harmful effect of SO\u2082 and its consequences on the placental function. We therefore elucidated the detrimental impact of SO\u2082 exposure on trophoblast cells and the possible health consequences that may contribute to pregnancy\u2010related complications as a result of compromised placental function.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16548-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec><jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Sulphur dioxide (SO\u2082) derivatives severely impaired trophoblasts migration, invasion and its ability of fusogenic differentiation.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>SO\u2082 derivatives appear to affect trophoblast cell interactions, thereby affecting its 3D organization and its capacity to form spheroids.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Mechanistic insight into these events indicates SO\u2082 derivatives mediated the induction of endoplasmic reticulum stress and inflammation, as well as altered histone\u20103\u00a0markers.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>We therefore conclude that SO\u2082 exposure has a detrimental effect on placental trophoblast cells. Long\u2010term exposure during pregnancy may result in an adverse outcome.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1113\/jp287409","type":"journal-article","created":{"date-parts":[[2025,2,22]],"date-time":"2025-02-22T00:24:27Z","timestamp":1740183867000},"page":"1263-1279","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Environmental pollutant SO\u2082 exposure affects trophoblast function involving an ER stress pathway"],"prefix":"10.1113","volume":"603","author":[{"given":"Sunil","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Biochemistry All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isha","family":"Goel","sequence":"additional","affiliation":[{"name":"Department of Psychiatry All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javed Ahsan","family":"Quadri","sequence":"additional","affiliation":[{"name":"Department of Anatomy All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rashmi","family":"Minocha","sequence":"additional","affiliation":[{"name":"Department of Biochemistry All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neha","family":"Kashyap","sequence":"additional","affiliation":[{"name":"Department of Biochemistry All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anubhuti","family":"Rana","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashok","family":"Ahirwar","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4114-9461","authenticated-orcid":false,"given":"Om Saswat","family":"Sahoo","sequence":"additional","affiliation":[{"name":"Department of Biotechnology National Institute of Technology Durgapur West Bengal India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruby","family":"Dhar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4757-8729","authenticated-orcid":false,"given":"Subhradip","family":"Karmakar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry All India Institute of Medical Sciences New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,2,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232012698"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-020-0372-6"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2010.12.002"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2010.12.002"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP281994"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01570-w"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2017.00078"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2015.10.005"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2022.113508"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12834"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0218799"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101545"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06298-9"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/00039896.1971.10665860"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2020.111872"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2023.1200330"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2203071119"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/451735"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03104-6"},{"issue":"12","key":"e_1_2_5_21_1","doi-asserted-by":"crossref","DOI":"10.1101\/cshperspect.a023010","article-title":"Molecular cross\u2010talk at the feto\u2013maternal interface","volume":"5","author":"Lash G. 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