{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T11:08:15Z","timestamp":1788433695188,"version":"build-2803163510"},"reference-count":64,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T00:00:00Z","timestamp":1733184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec><jats:title>Abstract<\/jats:title><jats:p>Pulmonary hypertension (PH) is a chronic, progressive disease characterized by pulmonary vascular remodelling, dyspnoea and exercise intolerance. Key facets of dyspnoea and exercise intolerance include skeletal and respiratory muscle contractile and metabolic disturbances; however, muscle perfusion during exercise has not been investigated. We hypothesized that diaphragm blood flow () would be increased and locomotory muscle  would be decreased during submaximal treadmill running in PH rats compared to healthy controls. Female Sprague\u2013Dawley rats were injected (<jats:sc>i.p.<\/jats:sc>) with monocrotaline to induce PH (<jats:italic>n<\/jats:italic>\u00a0=\u00a016), or a vehicle control (<jats:italic>n<\/jats:italic>\u00a0=\u00a015). Disease progression was monitored via echocardiography. When moderate disease severity was confirmed, maximal oxygen uptake () tests were performed. Rats were given &gt;24\u00a0h to recover, and then fluorescent microspheres were infused during treadmill running (20\u00a0m\/min, 10% grade; \u223c40\u201350% maximal speed attained during the  test) to determine tissue . In PH rats compared with healthy controls,  was lower (84 (7) <jats:italic>vs<\/jats:italic>. 67 (11) ml\/min\/kg; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), exercising diaphragm  was 35% higher and soleus  was 28% lower. Diaphragm  was negatively correlated with soleus  and  in PH rats. Furthermore, there was regional  redistribution in the diaphragm in PH compared to healthy rats, which may represent or underlie diaphragmatic weakness in PH. These findings suggest the presence of a pathological respiratory muscle blood flow steal phenomenon in PH and that this may contribute to the exercise intolerance reported in patients.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp16456-gra-0001-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec><jats:sec><jats:title>Key points<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Pulmonary hypertension (PH) impairs exercise tolerance, which is associated with skeletal and respiratory muscle dysfunction.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Increased work of breathing in PH may augment diaphragm blood flow and lower locomotory muscle blood flow during exercise, hindering exercise tolerance.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Our findings demonstrate that respiratory muscle blood flow is increased while the locomotory muscle is decreased in PH compared to healthy rats during exercise, suggesting that blood flow is preferentially redistributed to sustain ventilatory demand.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Furthermore, blood flow is regionally redistributed within the diaphragm in PH, which may underlie diaphragm dysfunction.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Greater respiratory muscle work at a given workload in PH commands higher respiratory muscle blood flow, impairing locomotory muscle oxygen delivery and compromising exercise tolerance, which may be improved by therapeutics which target the diaphragm vasculature.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1113\/jp287549","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T15:55:49Z","timestamp":1733241349000},"page":"337-351","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Skeletal and respiratory muscle blood flow redistribution during submaximal exercise in pulmonary hypertensive rats"],"prefix":"10.1113","volume":"603","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6467-5179","authenticated-orcid":false,"given":"Kiana M.","family":"Schulze","sequence":"first","affiliation":[{"name":"Department of Kinesiology Kansas State University  Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5110-270X","authenticated-orcid":false,"given":"Ramona E.","family":"Weber","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Kansas State University  Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4435-4022","authenticated-orcid":false,"given":"Andrew G.","family":"Horn","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Kansas State University  Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. Sue","family":"Hageman","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology Kansas State University  Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan J.","family":"Kenney","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Kansas State University  Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley J.","family":"Behnke","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Kansas State University  Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2441-3793","authenticated-orcid":false,"given":"David C.","family":"Poole","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Kansas State University  Manhattan KS USA"},{"name":"Department of Anatomy and Physiology Kansas State University  Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy I.","family":"Musch","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Kansas State University  Manhattan KS USA"},{"name":"Department of Anatomy and Physiology Kansas State University  Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,3]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062702"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1989.67.5.1855"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1984.246.1.H59"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.09-1140"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-010-1799-6"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2012-0506OC"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2007.06.014"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1979.47.6.1278"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114101"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP085182"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00358.2016"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.91566.2008"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.198382"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00164.2019"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00027909"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201003-0354OC"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1996.80.1.261"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-445X.1999.01852.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.4187\/respcare.02113"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00014.2016"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0160-5402(84)90050-0"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.14.3.413"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00212.2011"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP270818"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1997.82.5.1573"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2000.89.1.131"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00772.2014"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00320.2020"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00021.2022"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00099.2024"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00979.2001"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00408-013-9542-9"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/01902148.2017.1422157"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1982.243.2.H296"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00327.2021"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/HCR.0b013e3181d6f962"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2009.117168"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027993"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-014-1244-0"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0000000000000696"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00135.2012"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.618397"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.04.00095804"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1993.265.5.H1721"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1988.65.2.964"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1992.262.6.H1846"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1992.262.2.H411"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00943.2011"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005768-199706000-00003"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201402-0383OC"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1992.73.3.1067"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.89.4.1733"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2024.104686"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2023.1281715"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP089631"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2022.104334"},{"key":"e_1_2_5_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5687(95)00033-A"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1998.84.3.971"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01913-2018"},{"key":"e_1_2_5_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2019.02.003"},{"key":"e_1_2_5_62_1","doi-asserted-by":"publisher","DOI":"10.1161\/hc2901.093198"},{"key":"e_1_2_5_63_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.58.030196.000321"},{"key":"e_1_2_5_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ahj.2007.03.005"},{"key":"e_1_2_5_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/pul2.12358"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP287549","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T16:57:29Z","timestamp":1737046649000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/JP287549"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,3]]},"references-count":64,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1113\/JP287549"],"URL":"https:\/\/doi.org\/10.1113\/jp287549","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,3]]},"assertion":[{"value":"2024-08-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}