{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T02:17:21Z","timestamp":1790561841443,"version":"4.1.0"},"reference-count":92,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Urinary continence is maintained by the cooperation of smooth and striated muscles where urethral smooth muscle cell (USMC) contractions contribute to resting urethral tone during bladder filling, whereas striated muscle contracts in response to sudden abdominal pressure rises. Anoctamin\u20101 (ANO1), a calcium\u2010activated chloride channel (CaCC), has been identified as a potential regulator of USMC excitability. This review evaluates recent advances in understanding the cellular expression profile and functional contributions of ANO1 to USMC activity, for which there are significant species\u2010specific differences. Discrepancies in findings are often attributed to a lack of cell\u2010specific markers to map ANO1 expression in different cells and potentially off\u2010target effects of CaCC antagonists. In rabbits, ANO1 is localized in specialized interstitial cell of Cajal\u2010like cells (ICC\u2010LC) that provide a depolarizing stimulus to enhance USMC excitability and contractility. Conversely in mice, rats and sheep, ANO1 is expressed directly within USMC. Although early studies in multiple species using non\u2010specific antagonists suggested that CaCCs were critical for urethral tone and agonist\u2010evoked contractions, recent experiments utilizing more selective pharmacological tools (e.g. Ani9) and cell\u2010specific optogenetic sensors to distinguish ICC\u2010LC from USMC, indicate ANO1 may not significantly contribute to tone or neurotransmitter\u2010evoked contractions under normal physiological conditions, at least in mice and pigs. Although abundantly expressed, ANO1's role in urethra remains elusive. Emerging evidence suggests functional importance may manifest during pathophysiological states, such as urinary infections or metabolic disorders. This review summarizes these recent findings and proposes experiments that might fully elucidate the role of ANO1 channels in urethral smooth muscle.\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp70424-gra-0001-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>","DOI":"10.1113\/jp287614","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:13:16Z","timestamp":1773000796000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Update on cellular expression and function of ANO1 channels in urethral smooth muscle"],"prefix":"10.1113","author":[{"given":"Bernard T.","family":"Drumm","sequence":"first","affiliation":[{"name":"Department of Life &amp; Health Science, Smooth Muscle Research Centre Dundalk Institute of Technology  Dundalk Co. Louth Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neha","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Life &amp; Health Science, Smooth Muscle Research Centre Dundalk Institute of Technology  Dundalk Co. Louth Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,23]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1982.sp014182"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00585163"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.202213217"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.17383"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/MNH.0000000000000967"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00013.2017"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2409754122"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.14440\/bladder.2024.0029"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22992"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1468-7"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.097311"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.110932"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-013-1357-2"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1163518"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2022.01.042"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00174.2020"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP273618"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20888"},{"key":"e_1_2_3_20_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP281930"},{"key":"e_1_2_3_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/19336950.2025.2583809"},{"key":"e_1_2_3_22_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP284744"},{"key":"e_1_2_3_23_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP282570"},{"key":"e_1_2_3_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2014.241"},{"key":"e_1_2_3_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-39729-7"},{"key":"e_1_2_3_26_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP275719"},{"key":"e_1_2_3_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00520.2020"},{"key":"e_1_2_3_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2023.175677"},{"key":"e_1_2_3_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-017-2028-5"},{"key":"e_1_2_3_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28892"},{"key":"e_1_2_3_31_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.046607"},{"key":"e_1_2_3_32_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP278784"},{"key":"e_1_2_3_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15995"},{"key":"e_1_2_3_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00074.2009"},{"key":"e_1_2_3_35_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP284754"},{"key":"e_1_2_3_36_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP278888"},{"key":"e_1_2_3_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/function\/zqac041"},{"key":"e_1_2_3_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2016959117"},{"key":"e_1_2_3_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2024.102931"},{"key":"e_1_2_3_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-00953-z"},{"key":"e_1_2_3_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.459ae.x"},{"key":"e_1_2_3_42_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP284756"},{"key":"e_1_2_3_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15584.x"},{"key":"e_1_2_3_44_1","doi-asserted-by":"publisher","DOI":"10.2215\/CJN.04520413"},{"key":"e_1_2_3_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00413.2002"},{"key":"e_1_2_3_46_1","doi-asserted-by":"publisher","DOI":"10.4103\/tcmj.tcmj_221_22"},{"key":"e_1_2_3_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911935106"},{"key":"e_1_2_3_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/373347a0"},{"key":"e_1_2_3_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13010038"},{"key":"e_1_2_3_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13431"},{"key":"e_1_2_3_51_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.176198"},{"key":"e_1_2_3_52_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP277335"},{"key":"e_1_2_3_53_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.275107"},{"key":"e_1_2_3_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2011.01506.x"},{"key":"e_1_2_3_55_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP287315"},{"key":"e_1_2_3_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-024-03890-x"},{"key":"e_1_2_3_57_1","doi-asserted-by":"publisher","DOI":"10.1139\/y05-040"},{"key":"e_1_2_3_58_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00690.2020"},{"key":"e_1_2_3_59_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.15504"},{"key":"e_1_2_3_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13420"},{"key":"e_1_2_3_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2006.06617.x"},{"key":"e_1_2_3_62_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-7800.92525"},{"key":"e_1_2_3_63_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.189506"},{"key":"e_1_2_3_64_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.116657"},{"key":"e_1_2_3_65_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP288125"},{"issue":"1","key":"e_1_2_3_66_1","first-page":"35","article-title":"Increased complexity of Tmem16a\/Anoctamin 1 transcript alternative splicing","volume":"12","author":"O'Driscoll K. E.","year":"2011","journal-title":"BioMed Central Molecular Biology"},{"key":"e_1_2_3_67_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.273.5.C1588"},{"key":"e_1_2_3_68_1","doi-asserted-by":"publisher","DOI":"10.1152\/advan.00014.2018"},{"key":"e_1_2_3_69_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00514.2019"},{"key":"e_1_2_3_70_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1992.263.1.G115"},{"key":"e_1_2_3_71_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00344.2011"},{"key":"e_1_2_3_72_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00036.2022"},{"key":"e_1_2_3_73_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00006.2016"},{"key":"e_1_2_3_74_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP284745"},{"key":"e_1_2_3_75_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00037.2013"},{"key":"e_1_2_3_76_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2011.058248"},{"key":"e_1_2_3_77_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0155771"},{"key":"e_1_2_3_78_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.t01-2-00359.x"},{"key":"e_1_2_3_79_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-5895-1_6"},{"key":"e_1_2_3_80_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00085.2002"},{"key":"e_1_2_3_81_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2006.tb00399.x"},{"key":"e_1_2_3_82_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aaw7963"},{"key":"e_1_2_3_83_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP275478"},{"key":"e_1_2_3_84_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00070.2016"},{"key":"e_1_2_3_85_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP281686"},{"key":"e_1_2_3_86_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP289160"},{"key":"e_1_2_3_87_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2307513120"},{"key":"e_1_2_3_88_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.70007"},{"key":"e_1_2_3_89_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12072575"},{"key":"e_1_2_3_90_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020343"},{"key":"e_1_2_3_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mpsur.2017.03.002"},{"key":"e_1_2_3_92_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201812294"},{"key":"e_1_2_3_93_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00360.2014"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP287614","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/JP287614","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP287614","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:13:17Z","timestamp":1773000797000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/JP287614"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,23]]},"references-count":92,"alternative-id":["10.1113\/JP287614"],"URL":"https:\/\/doi.org\/10.1113\/jp287614","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,23]]},"assertion":[{"value":"2025-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"JP287614"}}