{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T08:01:43Z","timestamp":1785225703618,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"\n Abstract<\/jats:title>\n \n Chronic kidney disease (CKD) imposes a substantial health burden globally, with emerging evidence pointing to the significance of metabolic acidosis and low urinary NH\n 4<\/jats:sub>\n +<\/jats:sup>\n excretion resulting in poor CKD outcomes. The present study aims to identify in CKD patients, loss of function mutations in RhBG, one of the NH\n 3<\/jats:sub>\n \/NH\n 4<\/jats:sub>\n +<\/jats:sup>\n transporters in the collecting duct, and to show that NH\n 3<\/jats:sub>\n \/NH\n 4<\/jats:sub>\n +<\/jats:sup>\n transport is impaired by these mutations. Single nucleotide polymorphisms of RhBG associated with CKD occurrence were identified using ancestry\u2010stratified data from the Chronic Renal Insufficiency Cohort (CRIC) study. Functional analysis of NH\n 3<\/jats:sub>\n \/NH\n 4<\/jats:sub>\n +<\/jats:sup>\n transport was conducted in\n Xenopus<\/jats:italic>\n oocytes expressing RhBG protein or mutants. NH\n 3<\/jats:sub>\n and NH\n 4<\/jats:sub>\n +<\/jats:sup>\n transport was evaluated by electrophysiological measurements, including whole cell current, surface pH and intracellular pH. Our study identified six critical RhBG mutations associated with CKD. G86S and G86C inhibited the transport of NH\n 3<\/jats:sub>\n ; mutations G148R and G148W completely blocked transport of NH\n 3<\/jats:sub>\n and NH\n 4<\/jats:sub>\n +<\/jats:sup>\n , whereas T250A and T250S only inhibited NH\n 3<\/jats:sub>\n transport. Mutation T250M completely inhibited transport of both NH\n 3<\/jats:sub>\n and NH\n 4<\/jats:sub>\n +<\/jats:sup>\n . Our study identified critical rare non\u2010synonymous single nucleotide polymorphisms in RhBG associated with CKD and elucidated the impact of these variants on NH\n 3<\/jats:sub>\n \/NH\n 4<\/jats:sub>\n +<\/jats:sup>\n transport. These data are crucial to our understanding of how mutations can disrupt NH\n 3<\/jats:sub>\n \/NH\n 4<\/jats:sub>\n +<\/jats:sup>\n transport, potentially affecting kidney function in CKD patients susceptible to acidosis.\n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>\n \n Key points<\/jats:title>\n \n \n \n Acidosis and low urinary ammonium excretion contribute to poor outcomes in chronic kidney disease (CKD).<\/jats:p>\n <\/jats:list-item>\n \n This study investigates how the function of an ammonia transporter in renal collecting duct (RhBG) may contribute to CKD.<\/jats:p>\n <\/jats:list-item>\n \n Here, we report six rare RhBG mutations associated with CKD, identified using data from the Chronic Renal Insufficiency Cohort (CRIC) study.<\/jats:p>\n <\/jats:list-item>\n \n \n Using electrophysiological measurements, functional analysis in\n Xenopus<\/jats:italic>\n oocytes showed that these RhBG mutations disrupt ammonia transport, with some mutations affecting only NH\n 3<\/jats:sub>\n transport, whereas others affect both NH\n 3<\/jats:sub>\n and NH\n 4<\/jats:sub>\n +<\/jats:sup>\n transport.\n <\/jats:p>\n <\/jats:list-item>\n \n \n The results suggest that impaired ammonia transport by RhBG contributes to CKD, highlighting the need to understand mechanisms that link function (NH\n 3<\/jats:sub>\n \/NH\n 4<\/jats:sub>\n +<\/jats:sup>\n and acid\u2010base regulation) and genetic predisposition to CKD.\n <\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1113\/jp288958","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T06:41:22Z","timestamp":1770878482000},"page":"2302-2335","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mutations in ammonia transporter RhBG that impair NH\n 3<\/sub>\n \/NH\n 4<\/sub>\n +<\/sup>\n transport in patients with chronic kidney disease"],"prefix":"10.1113","volume":"604","author":[{"given":"He","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Medicine Tulane Medical School New Orleans LA USA"},{"name":"Department of Physiology Tulane Medical School New Orleans LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solange","family":"Abdulnour\u2010Nakhoul","sequence":"additional","affiliation":[{"name":"Department of Medicine Tulane Medical School New Orleans LA USA"},{"name":"Department of Physiology Tulane Medical School New Orleans LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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