{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T20:02:19Z","timestamp":1782936139727,"version":"3.54.5"},"reference-count":82,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T00:00:00Z","timestamp":1760227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"abstract":"Abstract<\/jats:title>Most vagal sensory afferents innervating the lower airways are activated by noxious stimuli including irritants (e.g. TRPV1 agonist capsaicin) and inflammatory mediators, causing nociceptive cardiorespiratory reflexes (e.g. cough, bronchospasm, changes in respiratory drive and heart rate). Vagal ganglia comprise embryologically distinct nodose and jugular neurons, but little is known of their specific contribution to nociceptive reflexes. Using a novel TRPV1Flp<\/jats:sup> mouse in combination with P2X2Cre<\/jats:sup>, Tac1Cre<\/jats:sup>, intersectional reporter mice, and adeno\u2010associated virus we mapped and modulated distinct nociceptive afferents. TRPV1+<\/jats:sup>P2X2+<\/jats:sup> neurons were found exclusively in the nodose ganglion and were activated by \u03b1\u03b2mATP and capsaicin, but rarely expressed Tac1. TRPV1+<\/jats:sup>P2X2+<\/jats:sup> fibres innervated the lungs (many projected into the alveoli) but not the trachea. Centrally they innervated the nucleus tractus solitarius (nTS). More than 90% of TRPV1+<\/jats:sup>Tac1+<\/jats:sup> neurons were found in the jugular ganglion and were activated by capsaicin but not \u03b1\u03b2mATP. TRPV1+<\/jats:sup>Tac1+<\/jats:sup> fibres innervated the lungs (although none projected into the alveoli) and the trachea submucosa. They terminated solely in the paratrigeminal complex (Pa5). Many TRPV1\u2013<\/jats:sup>Tac1+<\/jats:sup> neurons were found in both nodose and jugular ganglia that innervated the trachea and large pulmonary airways. These projected to both nTS and Pa5. Using intersectional chemogenetics, we selectively stimulated lower airway afferent subsets using intravenous injections of clozapine\u2010N<\/jats:italic>\u2010oxide. Activation of TRPV1+<\/jats:sup>, TRPV1+<\/jats:sup>P2X2+<\/jats:sup> or TRPV1+<\/jats:sup>Tac1+<\/jats:sup> fibres evoked bradycardia and bradypnoea. Activation of Tac1+<\/jats:sup> fibres evoked tachycardia and tachypnoea. Activation of vagal TRPV1\u2013<\/jats:sup>Tac1+<\/jats:sup> neurons only evoked tachycardia. These data show the distinct innervation patterns and reflex function of multiple nociceptive vagal afferent subsets.\nimage<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>Key points<\/jats:title>\nMost sensory innervation of the lower airways is provided by vagal afferents, which are divided into distinct nodose and jugular subsets.<\/jats:p><\/jats:list-item>\nOur intersectional approach showed that co\u2010expression of P2X2 and TRPV1 labelled nodose nociceptors, that co\u2010expression of Tac1 and TRPV1 labelled jugular nociceptors, and that Tac1 expression with TRPV1 labelled a mixed population of nodose and jugular neurons.\n<\/jats:p><\/jats:list-item>\nNodose nociceptors, jugular nociceptors and vagal TRPV1\u2010negative Tac1\u2010positive (TRPV1\u2013<\/jats:sup>Tac1+<\/jats:sup>) afferents project to distinct medulla subnuclei, and innervate different regions and tissues within the lower airways.<\/jats:p><\/jats:list-item>\nChemogenetic activation (via intravenous clozapine\u2010N<\/jats:italic>\u2010oxide) of nodose and jugular nociceptors evoked bradypnoea and bradycardia, whereas activation of TRPV1\u2010negative Tac1\u2010positive (TRPV1\u2013<\/jats:sup>Tac1+<\/jats:sup>) afferents evoked tachycardia.<\/jats:p><\/jats:list-item>\nThese data show that genetically distinct vagal afferent subsets have distinct innervation patterns and reflex function.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1113\/jp289279","type":"journal-article","created":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T22:16:56Z","timestamp":1760307416000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Anatomical and functional mapping of vagal nociceptive sensory nerve subsets innervating the mouse lower airways by intersectional genetics"],"prefix":"10.1113","author":[{"given":"Mayur 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nika","family":"Pavelkova","sequence":"additional","affiliation":[{"name":"Molecular Pharmacology & Physiology, Morsani College of Medicine University of South Florida Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay S.","family":"Nair","sequence":"additional","affiliation":[{"name":"Molecular Pharmacology & Physiology, Morsani College of Medicine University of South Florida Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7186-977X","authenticated-orcid":false,"given":"Teresa S.","family":"Darcey","sequence":"additional","affiliation":[{"name":"Molecular Pharmacology & Physiology, Morsani College of Medicine University of South Florida Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina V.","family":"Lurye","sequence":"additional","affiliation":[{"name":"Molecular Pharmacology & Physiology, Morsani College of Medicine University of South Florida Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghana","family":"Madaram","sequence":"additional","affiliation":[{"name":"Molecular Pharmacology & Physiology, Morsani College of Medicine University of South Florida Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jailene","family":"Fiallo","sequence":"additional","affiliation":[{"name":"Molecular Pharmacology & Physiology, Morsani College of Medicine University of South Florida Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen H.","family":"Hadley","sequence":"additional","affiliation":[{"name":"Molecular Pharmacology & Physiology, Morsani College of Medicine University of South Florida Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5056-2699","authenticated-orcid":false,"given":"Thomas E.","family":"Taylor\u2010Clark","sequence":"additional","affiliation":[{"name":"Molecular Pharmacology & Physiology, Morsani College of Medicine University of South Florida Tampa FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,12]]},"reference":[{"issue":"1","key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1006\/dbio.2001.0156","article-title":"Vertebrate cranial placodes I. Embryonic induction","volume":"232","author":"Baker C. V.","year":"2001","journal-title":"Developmental Biology"},{"issue":"4","key":"e_1_2_7_3_1","doi-asserted-by":"crossref","DOI":"10.1084\/jem.20201637","article-title":"A TRPA1 inhibitor suppresses neurogenic inflammation and airway contraction for asthma treatment","volume":"218","author":"Balestrini A.","year":"2021","journal-title":"Journal of Experimental Medicine"},{"issue":"4","key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1038\/nm.4501","article-title":"Nociceptor sensory neurons suppress neutrophil and \u03b3\u03b4 T cell responses in bacterial lung infections and lethal pneumonia","volume":"24","author":"Baral P.","year":"2018","journal-title":"Nature Medicine"},{"issue":"17","key":"e_1_2_7_5_1","doi-asserted-by":"crossref","first-page":"3072","DOI":"10.1002\/cne.25398","article-title":"Analysis of the distribution of vagal afferent projections from different peripheral organs to the nucleus of the solitary tract in rats","volume":"530","author":"Bassi J. K.","year":"2022","journal-title":"Journal of Comparative Neurology"},{"issue":"5","key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"1899","DOI":"10.1172\/JCI34192","article-title":"TRPA1 is a major oxidant sensor in murine airway sensory neurons","volume":"118","author":"Bessac B. F.","year":"2008","journal-title":"Journal of Clinical Investigation"},{"issue":"1","key":"e_1_2_7_7_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1113\/jphysiol.1991.sp018740","article-title":"Neurones in commissural nucleus tractus solitarii required for full expression of the pulmonary C fibre reflex in rat","volume":"441","author":"Bonham A. C.","year":"1991","journal-title":"The Journal of Physiology"},{"issue":"6","key":"e_1_2_7_8_1","doi-asserted-by":"crossref","first-page":"2642","DOI":"10.1152\/jappl.2001.91.6.2642","article-title":"Multiple mechanisms of reflex bronchospasm in guinea pigs","volume":"91","author":"Canning B. 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L.","year":"2008","journal-title":"American Journal of Physiology\u2010Regulatory, Integrative and Comparative Physiology"},{"key":"e_1_2_7_17_1","first-page":"1","article-title":"Afferent vagal C fibre innervation of the lungs and airways and its functional significance","volume":"99","author":"Coleridge J. C.","year":"1984","journal-title":"Reviews of Physiology, Biochemistry and Pharmacology"},{"issue":"2","key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1016\/j.cell.2018.06.035","article-title":"A suite of transgenic driver and reporter mouse lines with enhanced brain\u2010cell\u2010type targeting and functionality","volume":"174","author":"Daigle T. 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J.","year":"2020","journal-title":"The Journal of Physiology"},{"issue":"7","key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1038\/nmeth.2996","article-title":"Targeting cells with single vectors using multiple\u2010feature boolean logic","volume":"11","author":"Fenno L. E.","year":"2014","journal-title":"Nature Methods"},{"issue":"9","key":"e_1_2_7_24_1","doi-asserted-by":"crossref","first-page":"1734","DOI":"10.1038\/s41593-024-01712-5","article-title":"A vagal\u2010brainstem interoceptive circuit for cough\u2010like defensive behaviors in mice","volume":"27","author":"Gannot N.","year":"2024","journal-title":"Nature Neuroscience"},{"issue":"2","key":"e_1_2_7_25_1","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1093\/toxsci\/kfn005","article-title":"Cardiac oxidative stress and electrophysiological changes in rats exposed to concentrated ambient particles are mediated by TRP\u2010dependent pulmonary reflexes","volume":"102","author":"Ghelfi E.","year":"2008","journal-title":"Toxicological Sciences"},{"issue":"2","key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1152\/jappl.1984.57.2.562","article-title":"Pulmonary C\u2010fibers evoke both apnea and tachypnea of pulmonary chemoreflex","volume":"57","author":"Green J. 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H.","year":"2020","journal-title":"eNeuro"},{"issue":"2","key":"e_1_2_7_32_1","first-page":"ENEURO.0026","article-title":"Mapping of the sensory innervation of the mouse lung by specific vagal and dorsal root ganglion neuronal subsets","volume":"9","author":"Kim S. H.","year":"2022","journal-title":"eNeuro"},{"issue":"5","key":"e_1_2_7_33_1","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/j.neuron.2016.07.044","article-title":"Coupled activation of primary sensory neurons contributes to chronic pain","volume":"91","author":"Kim Y. 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Y.","year":"2014","journal-title":"Comprehensive Physiology"},{"issue":"6548","key":"e_1_2_7_41_1","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1038\/377432a0","article-title":"Coexpression of P2\u00d72 and P2\u00d73 receptor subunits can account for ATP\u2010gated currents in sensory neurons","volume":"377","author":"Lewis C.","year":"1995","journal-title":"Nature"},{"issue":"9","key":"e_1_2_7_42_1","doi-asserted-by":"crossref","first-page":"L941","DOI":"10.1152\/ajplung.00366.2011","article-title":"TRPV1 induction in airway vagal low\u2010threshold mechanosensory neurons by allergen challenge and neurotrophic factors","volume":"302","author":"Lieu T. 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