{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:18:54Z","timestamp":1792801134099,"version":"4.5.0"},"reference-count":41,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM125503"],"award-info":[{"award-number":["P20GM125503"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR075794\u201001A1\/AR\/NIAMS"],"award-info":[{"award-number":["R01AR075794\u201001A1\/AR\/NIAMS"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"\n Abstract<\/jats:title>\n \n We investigated the prophylactic effects of exercise before and during cancer cachexia (CC) using a model designed to mimic endurance and resistance (i.e., concurrent) adaptations. Male and female\n Balb\/c<\/jats:italic>\n mice were randomly assigned to exercise or control groups whereby exercise groups were subjected to an 8\u2010week voluntary progressive weighted wheel running (PoWeR) programme of habitual loading\u2010mediated physical activity beginning at 8\u00a0weeks of age. At 16\u00a0weeks of age, mice were injected bilaterally with colon\u201026 adenocarcinoma (C26) cells or phosphate\u2010buffered saline, and exercise training was maintained throughout disease progression. Twenty\u2010five days post\u2010tumour induction, we assessed whole\u2010body and muscle phenotype, muscle protein synthesis,\n a priori<\/jats:italic>\n targeted gene expression, and transcriptomic adaptations via RNA sequencing. PoWeR training preserved skeletal muscle mass across nearly all muscle groups and maintained tumour\u2010free body and cardiac mass. Muscle mass adaptations related to running volume, and running distance relative to controls were not appreciably reduced by tumour status. Tumour burden was reduced after \u223c11.5\u00a0weeks of PoWeR compared to sedentary, but this was not explanatory for muscle adaptations. PoWeR induced a faster\u2010to\u2010slower muscle fibre type transition in the gastrocnemius and suppressed key protein turnover markers (\n Redd1<\/jats:italic>\n ,\n Murf1<\/jats:italic>\n ,\n Atrogin<\/jats:italic>\n ,\n Ubc<\/jats:italic>\n ,\n Gadd45a<\/jats:italic>\n ) as well as the mitophagy\u2010related marker\n Bnip3<\/jats:italic>\n in tumour\u2010bearing muscle; 24\u00a0h muscle protein synthesis remained stable. PoWeR counteracted tumour\u2010induced impairments in the muscle mitochondrial\u2010 and metabolic\u2010related transcriptome. Collectively, physical activity prior to and during cancer preserves muscle mass, reduces tumour growth and mitigates molecular drivers of CC, underscoring its preventive and therapeutic potential as a lifestyle intervention.\n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>\n \n Key points<\/jats:title>\n \n \n \n Cancer cachexia (CC) is a severe, multifactorial syndrome with limited effective therapies.<\/jats:p>\n <\/jats:list-item>\n \n Exercise training has emerged as a promising non\u2010pharmacological approach to mitigate CC.<\/jats:p>\n <\/jats:list-item>\n \n Concurrent endurance and resistance training, initiated prior to and maintained during cancer, preserves skeletal muscle mass and reduces tumour burden in C26 colorectal tumour\u2010bearing mice.<\/jats:p>\n <\/jats:list-item>\n \n Concurrent exercise training suppresses key mitochondrial\u2010 and metabolic\u2010related molecular mediators of CC.<\/jats:p>\n <\/jats:list-item>\n \n Concurrent exercise training may serve as a preventive and therapeutic non\u2010pharmacological strategy against CC.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1113\/jp290740","type":"journal-article","created":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T12:12:35Z","timestamp":1780575155000},"page":"6028-6056","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exercise training prior to and during cancer in mice preserves muscle mass, reduces tumour weight and suppresses molecular mediators of cachexia"],"prefix":"10.1113","volume":"604","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6753-5945","authenticated-orcid":false,"given":"Stavroula","family":"Tsitkanou","sequence":"first","affiliation":[{"name":"Cachexia Research Laboratory, Exercise Science Research Center, Department of Health, Human Performance and Recreation University of Arkansas Fayetteville AR USA"},{"name":"Department of Neurology, Beth Israel Deaconess Medical Center Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter J.","family":"Koopmans","sequence":"additional","affiliation":[{"name":"Molecular Muscle Mass Regulation Laboratory, Exercise Science Research Center, Department of Health, Human Performance and Recreation University of Arkansas Fayetteville AR USA"},{"name":"Cell and Molecular Biology Program University of Arkansas Fayetteville AR USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Regina","family":"Cabrera","sequence":"additional","affiliation":[{"name":"Cachexia Research Laboratory, Exercise Science Research Center, Department of Health, Human Performance and Recreation University of Arkansas Fayetteville AR 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Laboratory, Exercise Science Research Center, Department of Health, Human Performance and Recreation University of Arkansas Fayetteville AR USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francielly","family":"Morena","sequence":"additional","affiliation":[{"name":"Cachexia Research Laboratory, Exercise Science Research Center, Department of Health, Human Performance and Recreation University of Arkansas Fayetteville AR USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine University of Kentucky Lexington KY USA"},{"name":"Department of Internal Medicine, College of Medicine University of Kentucky Lexington KY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1919-5907","authenticated-orcid":false,"given":"Tyrone 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