{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T23:35:31Z","timestamp":1789601731206,"version":"build-2803163510"},"reference-count":61,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Obesity during pregnancy is at pandemic proportions and predisposes women to pre\u2010 and postnatal cardiovascular dysfunction. The mechanisms underlying this maternal cardiovascular vulnerability remain unclear, partly due to a lack of translatable models capable of longitudinal\n                      <jats:italic>in vivo<\/jats:italic>\n                      cardiovascular monitoring. Here, we characterize a novel ovine model of maternal diet\u2010induced obesity during pregnancy. Ewes were fed a control (CON) or obesogenic (OB;\n                      <jats:italic>ad libitum<\/jats:italic>\n                      concentrates) diet for 60\u00a0days pre\u2010pregnancy and throughout gestation. Pregnant ewes were surgically instrumented with vascular catheters and Transonic flow probes using the wireless CamDAS system, which measured maternal cardiovascular function near term in free\u2010moving ewes. Uterine artery vasoreactivity was assessed\n                      <jats:italic>ex vivo<\/jats:italic>\n                      by\n                      <jats:italic>in vitro<\/jats:italic>\n                      wire myography. OB ewes entered pregnancy 30% heavier than controls (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.003) and were hyperglycaemic, hyperinsulinaemic and hyperlipidaemic during pregnancy, relative to CON ewes (all\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). OB ewes had elevated haematocrit and haemoglobin across pregnancy, and were hypertensive near term, with an increase in basal femoral artery blood flow, and elevated peripheral oxygen and glucose delivery (all\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). OB mothers carrying a female fetus showed increased uterine artery vascular resistance\n                      <jats:italic>in vivo<\/jats:italic>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.005) and reduced smooth muscle\u2010dependent vasorelaxation\n                      <jats:italic>ex vivo<\/jats:italic>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05) relative to CON. Conversely, OB mothers carrying a male fetus showed greater NO\u2010independent mechanisms mediating the uterine vasodilator response to methacholine\n                      <jats:italic>ex vivo<\/jats:italic>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.001). Collectively, this study characterizes a robust model of maternal obesity during pregnancy that offers clinical translational potential and highlights fetal sex\u2010specific changes to uterine artery function.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp70603-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>Obesity during pregnancy is increasingly common and predisposes women to cardiovascular dysfunction during pregnancy and long after birth, but the specific mechanisms underlying this remain unclear.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>We developed a novel ovine model of diet\u2010induced obesity during pregnancy that displays maternal hypertension, elevated haemoglobin, metabolic dysfunction, and alterations in uterine and peripheral blood flow and nutrient delivery near term.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            Mothers with obesity carrying a female fetus had elevated uterine vascular resistance\n                            <jats:italic>in vivo<\/jats:italic>\n                            and reduced uterine artery smooth muscle\u2010dependent vasodilator reactivity\n                            <jats:italic>ex vivo<\/jats:italic>\n                            .\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            Mothers with obesity carrying a male fetus showed no effect on uterine vascular resistance\n                            <jats:italic>in vivo<\/jats:italic>\n                            , but greater NO\u2010independent mechanisms mediating the uterine vasodilator response to methacholine\n                            <jats:italic>ex vivo<\/jats:italic>\n                            .\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>These findings highlight that fetal sex may influence maternal cardiovascular function during obese pregnancy.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp290766","type":"journal-article","created":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T01:50:19Z","timestamp":1779501019000},"page":"5011-5030","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Maternal cardiometabolic dysfunction and fetal sex\u2010specific alterations to uterine vascular reactivity in an ovine model of obesity during pregnancy"],"prefix":"10.1113","volume":"604","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3024-9803","authenticated-orcid":false,"given":"Rachael C.","family":"Crew","sequence":"first","affiliation":[{"name":"Department of Physiology, Development and Neuroscience University of Cambridge  Cambridge UK"},{"name":"Department of Obstetrics and Gynaecology University of Cambridge  Cambridge UK"},{"name":"School of Human Sciences University of Western Australia  Perth Western Australia Australia"},{"name":"Loke Centre for Trophoblast Research University of Cambridge  Cambridge UK"},{"name":"Strategic Research Initiative in Reproduction University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7763-7322","authenticated-orcid":false,"given":"Anna L. 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