{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:04:52Z","timestamp":1790017492234,"version":"4.0.1"},"reference-count":69,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"abstract":"\n Abstract<\/jats:title>\n \n Central respiratory chemoreceptors (CRCs) are critically important for maintaining normal systemic levels of pH and PCO\n 2<\/jats:sub>\n . Their specific identity has been controversial. It is widely acknowledged that CRCs must respond to small changes in pH via cell\u2010autonomous (intrinsic) mechanisms. However most studies designed to identify CRCs have only blocked a limited subset of synaptic mechanisms. Here we used patch\u2010clamp recordings to compare chemosensitivity of two candidates for CRCs: (1)\n Phox2b<\/jats:italic>\n \u2010expressing neurones of the retrotrapezoid nucleus (RTN) and (2) serotonin (5\u2010HT)\u2010producing neurones of the medullary raphe nuclei. We used acute dissociation to ensure responses were intrinsic. In response to a change in CO\n 2<\/jats:sub>\n from 5% to 9% (pH 7.4 to \u22487.2) 48% of medullary 5\u2010HT neurones (\n n<\/jats:italic>\n \u00a0=\u00a0118) increased their firing rate by more than 20% with a mean increase of 139%, whereas 16% of RTN neurones (\n n<\/jats:italic>\n \u00a0=\u00a093) increased their firing rate by more than 20% with a mean increase of 46%. RTN neurones that expressed Neuromedin B (\n Nmb<\/jats:italic>\n ) (a proposed biomarker of RTN CRCs) were not more likely to have a larger pH response. After 3 days in culture many RTN neurones began to receive excitatory synaptic drive from 5\u2010HT neurones and also responded to acidosis. These results support the conclusion that a subset of medullary 5\u2010HT neurones are CRCs. Many RTN neurones may play a role in integration and relay of pH information from 5\u2010HT neurones and other chemoreceptor sites but contribute less to direct pH sensation.\n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>\n \n Key points<\/jats:title>\n \n \n \n \n Central respiratory chemoreceptors in the brainstem detect changes in CO\n 2<\/jats:sub>\n and pH via intrinsic mechanisms and induce changes in breathing to maintain pH homoeostasis. The identity of these cells is controversial.\n <\/jats:p>\n <\/jats:list-item>\n \n We measured the pH response of two prominent candidates for these chemoreceptors after they were acutely isolated from all other cells to ensure that their responses were intrinsic.<\/jats:p>\n <\/jats:list-item>\n \n A small percentage of neurones from the retrotrapezoid nucleus had a small response to acidosis. A much larger percentage of serotonin neurones were stimulated by acidosis with a large increase in firing rate.<\/jats:p>\n <\/jats:list-item>\n \n Over the first few days in cultured neuronal preparations serotonin neurones formed synapses on retrotrapezoid neurones and stimulated them in response to acidosis.<\/jats:p>\n <\/jats:list-item>\n \n These results reveal that a large subset of serotonin neurones have properties consistent with central chemoreceptors, whereas a small number of retrotrapezoid neurones have a small response, and may be more important as relays of chemoreceptor information rather than as direct sensors of pH themselves.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1113\/jp290796","type":"journal-article","created":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T01:19:36Z","timestamp":1781918376000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Intrinsic pH chemosensitivity of acutely dissociated medullary serotonergic and retrotrapezoid neurones"],"prefix":"10.1113","author":[{"given":"Yuanming","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Neurology University of Iowa Iowa City Iowa USA"},{"name":"Iowa Neuroscience Institute University of Iowa Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9209-841X","authenticated-orcid":false,"given":"Eduardo","family":"Bravo","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Iowa Iowa City Iowa USA"},{"name":"Iowa Neuroscience Institute University of Iowa Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5696-1554","authenticated-orcid":false,"given":"George Bradley","family":"Richerson","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Iowa Iowa City Iowa USA"},{"name":"Department of Molecular Physiology & Biophysics University of Iowa Iowa City Iowa USA"},{"name":"Iowa Neuroscience Institute University of Iowa Iowa City Iowa USA"},{"name":"Neurology Service Veteran's Affairs Medical Center Iowa City Iowa USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,19]]},"reference":[{"issue":"18","key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"5806","DOI":"10.1523\/JNEUROSCI.1106-09.2009","article-title":"Photostimulation of retrotrapezoid nucleus phox2b\u2010expressing neurons in vivo produces long\u2010lasting activation of breathing in rats","volume":"29","author":"Abbott S. 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