{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T07:47:54Z","timestamp":1785743274126,"version":"3.56.0"},"reference-count":48,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["RE\/24\/130023"],"award-info":[{"award-number":["RE\/24\/130023"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["RG\/F\/22\/110078"],"award-info":[{"award-number":["RG\/F\/22\/110078"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>Cardiac optical mapping in fully contracting hearts is limited by motion artefacts, leading most investigations to rely on excitation\u2013contraction uncoupling despite its impact on physiological relevance. Although motion tracking and ratiometric imaging have each been used to reduce motion artefacts, and their combined use has been demonstrated with excitation ratiometry, systematic validation of emission ratiometry combined with motion tracking against an established electrophysiological reference remains limited.<\/jats:p>\n                    <jats:p>\n                      In this study, we integrated emission ratiometry with two\u2010dimensional, marker\u2010free motion tracking to suppress motion\u2010related artefacts during optical mapping of Langendorff\u2010perfused rabbit hearts, leveraging the advantage that emission ratiometry derives both signals from the same excitation pathway and thereby reduces sensitivity to motion\u2010related photometric fluctuations in fluorescence intensity. Motion\u2010corrected optical action potentials were quantitatively validated against simultaneously recorded monophasic action potentials (MAPs), with optical signals extracted from regions adjacent to the MAP electrode. Compared with raw recordings and with either method applied alone, the combined approach produced the closest agreement with MAP\u2010derived repolarization measurements. Bland\u2013Altman analysis indicated no systematic bias at APD\n                      <jats:sub>70<\/jats:sub>\n                      and APD\n                      <jats:sub>80<\/jats:sub>\n                      , with bias confidence intervals including zero. Cumulative probability analysis revealed a marked improvement in precision following combined motion correction, with the proportion of optical action potential duration (APD) measurements within \u00b110\u00a0ms of MAP increasing from 16.9%, 29.2% and 26.2% in raw recordings to 75.4%, 87.7% and 80% for APD\n                      <jats:sub>50<\/jats:sub>\n                      , APD\n                      <jats:sub>70<\/jats:sub>\n                      and APD\n                      <jats:sub>80<\/jats:sub>\n                      , respectively.\n                    <\/jats:p>\n                    <jats:p>\n                      Collectively, these findings establish the integrated motion\u2010correction strategy as a validated framework for accurate quantification of action potentials in contracting hearts, supporting more physiologically relevant optical mapping.\n                      <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                        <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/tjp70655-gra-0001-m.png\">\n                          <jats:alt-text>image<\/jats:alt-text>\n                        <\/jats:graphic>\n                      <\/jats:boxed-text>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>New &amp; noteworthy<\/jats:title>\n                    <jats:p>While motion tracking and ratiometric approaches have previously been used to mitigate motion artefacts in optical mapping, this study is the first to integrate emission ratiometry with fully marker\u2010free motion tracking and to provide systematic validation against simultaneously recorded monophasic action potentials in freely contracting isolated rabbit hearts. This validated framework enables accurate optical assessment of cardiac electrical activity under physiologically relevant conditions without mechanical stabilization or excitation\u2013contraction uncoupling.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/jp291400","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:30:14Z","timestamp":1781656214000},"page":"5758-5776","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Combined emission ratiometry and motion tracking for optical mapping of contracting hearts: Validation with monophasic action potentials"],"prefix":"10.1113","volume":"604","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3708-0329","authenticated-orcid":false,"given":"Vineesh","family":"Kappadan","sequence":"first","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0424-8534","authenticated-orcid":false,"given":"Zhen","family":"Hua","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"},{"name":"Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna B.","family":"Tonko","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najmah","family":"Mohamed","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8754-4964","authenticated-orcid":false,"given":"Jan","family":"Lebert","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute University of California  San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paraskevas","family":"Efstathiou","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasser","family":"Abdelghani","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danya","family":"Agha\u2010Jaffar","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4923-2529","authenticated-orcid":false,"given":"Anies","family":"Sohi","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5354-5833","authenticated-orcid":false,"given":"Xianbo","family":"Sun","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Baptiste","family":"Guichard","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"},{"name":"Institut Cl\u00ednic Cardiovascular (ICCV) Hospital Cl\u00ednic, Universitat de Barcelona  Barcelona Catalonia Spain"},{"name":"Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS)  Barcelona Catalonia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3594-4717","authenticated-orcid":false,"given":"Jan","family":"Christoph","sequence":"additional","affiliation":[{"name":"Cardiovascular Research Institute University of California  San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas S.","family":"Peters","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8681-4368","authenticated-orcid":false,"given":"Fu Siong","family":"Ng","sequence":"additional","affiliation":[{"name":"National Heart and Lung Institute (NHLI) Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1099800409338516"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1109\/TBME.2011.2148719"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00234.2004"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8167.2000.tb00066.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2012.069369"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.00171.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","unstructured":"Chowdhary S. Lebert J. Dickman S. Manetta M. Gordon C. &Christoph J.(2026).Panoramic voltage\u2010sensitive optical mapping of contracting hearts using cooperative multiview motion tracking with 12 cameras.The Journal of Physiology. Advance online publication.https:\/\/doi.org\/10.1113\/JP290533","DOI":"10.1113\/JP290533"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01483"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2017.09.015"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.90.3.1469"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000130529.18016.35"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hrthm.2006.12.047"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(96)00074-0"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(99)00390-9"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6107(03)00013-0"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP273873"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03279-y"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvaa141"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.247494"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00169.2025"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP283683"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00464"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2000.279.3.H1421"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6107(03)00022-1"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1540-8167.2003.02077.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP085042"},{"key":"e_1_2_7_28_1","unstructured":"Lebert J. &Christoph J.(2023).optimap: An open\u2010source library for the processing of fluorescence video data. [Accessed March 16 2026].https:\/\/doi.org\/10.5281\/zenodo.8361821"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.787627"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvz039"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-012-1135-6"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00711.2011"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-025-09240-z"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(96)00054-5"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370219894942"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00036.2019"},{"issue":"7","key":"e_1_2_7_37_1","first-page":"1424","article-title":"Distinct electrogram features and ventricular arrhythmia induction modes between repolarization and conduction heterogeneities","volume":"10","author":"Renard E.","year":"2024","journal-title":"Journal of the American College of Cardiology: Clinical Electrophysiology"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1109\/TBME.2004.840464"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.191.4226.485"},{"key":"e_1_2_7_40_1","doi-asserted-by":"crossref","unstructured":"Sobitov I. Ritzenhoff K. Gaulrapp M. Becker L. Liutkute A. Seibertz F. Fakuade F. E. Mason F. E. &Voigt N.(2026).Blebbistatin reduces calcium buffering in cardiomyocytes: Consequences for cellular electrophysiology.The Journal of Physiology. Advance online publication.https:\/\/doi.org\/10.1113\/JP287545","DOI":"10.1113\/JP287545"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1109\/IEMBS.2009.5333531"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1088\/0967-3334\/29\/2\/009"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00477.2021"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2008.02.021"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00556.2014"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00696.2013"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(96)00089-2"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2016.03.043"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2023.09.017"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP291400","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/JP291400","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/JP291400","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T07:35:05Z","timestamp":1785742505000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/JP291400"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,16]]},"references-count":48,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1113\/JP291400"],"URL":"https:\/\/doi.org\/10.1113\/jp291400","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,16]]},"assertion":[{"value":"2026-03-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}