{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:29:06Z","timestamp":1790393346724,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL158846"],"award-info":[{"award-number":["HL158846"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL155180"],"award-info":[{"award-number":["HL155180"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL166411"],"award-info":[{"award-number":["HL166411"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["24CDA1269410"],"award-info":[{"award-number":["24CDA1269410"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["25CDA1435154"],"award-info":[{"award-number":["25CDA1435154"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["24POST1191382"],"award-info":[{"award-number":["24POST1191382"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"\n Abstract<\/jats:title>\n \n Polycystin 1 (PKD1) is a large transmembrane protein expressed by the\n Pkd1<\/jats:italic>\n gene. Previous data obtained using germline non\u2010specific knockout mice suggested that PKD1 regulates arterial smooth muscle cell contractility. Here, we generated tamoxifen\u2010inducible, smooth muscle cell\u2010specific (\n Myh11<\/jats:italic>\n \u2010\n \n CreER\n T2<\/jats:sup>\n ) Pkd1\n <\/jats:italic>\n knockout (\n Pkd1\u00a0<\/jats:italic>\n smKO) mice to further investigate the functional significance of PKD1 in arterial myocytes. Reverse\u2010transcription polymerase chain reaction, western blotting and immunofluorescence imaging demonstrated that PKD1\u00a0message and protein were expressed in arterial myocytes of male control (\n \n Pkd1\n fl\/fl<\/jats:sup>\n <\/jats:italic>\n ) mice but absent in arterial myocytes of\n Pkd1\u00a0<\/jats:italic>\n smKO mice. Polycystin 2 (PKD2), a transient receptor potential channel which can couple to PKD1, was similarly expressed in arteries of\n \n Pkd1\n fl\/fl<\/jats:sup>\n <\/jats:italic>\n and\n Pkd1\u00a0<\/jats:italic>\n smKO mice. Kidney anatomy and glomerular and proximal tubule sizes were unaltered in\n Pkd1\u00a0<\/jats:italic>\n smKO mice. Intravascular pressures of between 10 and 125\u00a0mmHg similarly constricted resistance\u2010size mesenteric and gastrocnemius muscle arteries of\n \n Pkd1\n fl\/fl<\/jats:sup>\n <\/jats:italic>\n and\n Pkd1<\/jats:italic>\n \u00a0smKO mice, regardless of whether experiments were performed 14 or 28\u00a0days after tamoxifen administration. Membrane depolarization (60\u00a0m\n m<\/jats:sc>\n K\n +<\/jats:sup>\n ), phenylephrine and angiotensin II also comparably constricted arteries of\n \n Pkd1\n fl\/fl<\/jats:sup>\n <\/jats:italic>\n and\n Pkd1\u00a0<\/jats:italic>\n smKO mice. Acetylcholine, an endothelium\u2010dependent vasodilator, stimulated equivalent dilatation in\n \n Pkd1\n fl\/fl<\/jats:sup>\n <\/jats:italic>\n and\n Pkd1\u00a0<\/jats:italic>\n smKO mouse arteries. Consistent with these results, blood pressure and heart rate were similar in\n \n Pkd1\n fl\/fl<\/jats:sup>\n <\/jats:italic>\n and\n Pkd1\u00a0<\/jats:italic>\n smKO mice. In summary, these data indicate that PKD1 is expressed in systemic artery myocytes, but it does not contribute to pressure\u2010, depolarization\u2010 or agonist\u2010induced vasoconstriction, or regulate blood pressure.\n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>\n \n Key points<\/jats:title>\n \n \n \n Polycystin 1 (PKD1) is a large membrane protein expressed in several cell types, including vascular smooth muscle cells. Previous data obtained using germline non\u2010specific knockout mice proposed that PKD1 regulates arterial smooth muscle cell contractility.<\/jats:p>\n <\/jats:list-item>\n \n We generated tamoxifen\u2010inducible smooth muscle cell\u2010specific PKD1 knockout mice to further investigate the regulation of arterial smooth muscle cell contractility by PKD1.<\/jats:p>\n <\/jats:list-item>\n \n Our data show that PKD1 is expressed in smooth muscle cells, but it does not contribute to intravascular pressure\u2010, agonist\u2010 or depolarization\u2010induced constriction, nor does its knockout alter endothelial cell\u2010dependent vasodilatation in resistance\u2010size systemic arteries of male mice.<\/jats:p>\n <\/jats:list-item>\n \n Smooth muscle cell PKD1 does not regulate blood pressure or heart rate.<\/jats:p>\n <\/jats:list-item>\n \n We conclude that PKD1 is dispensable for physiological regulation of arterial smooth muscle cell contractility.<\/jats:p>\n <\/jats:list-item>\n <\/jats:list>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1113\/jp291612","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T15:32:48Z","timestamp":1783524768000},"page":"6348-6368","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Smooth muscle cell polycystin 1 (PKD1) is dispensable for physiological regulation of arterial contractility"],"prefix":"10.1113","volume":"604","author":[{"given":"Ang\u00e9lica K.","family":"Bernardelli","sequence":"first","affiliation":[{"name":"Department of Physiology, College of Medicine University of Tennessee Health Science Center Memphis Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2937-575X","authenticated-orcid":false,"given":"Roberto B.","family":"Pontes","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine University of Tennessee Health Science Center Memphis Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1831-7842","authenticated-orcid":false,"given":"Alejandro","family":"Mata\u2010Daboin","sequence":"additional","affiliation":[{"name":"Department of Physiology, College of Medicine University of Tennessee Health Science Center Memphis Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tessa A. 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