{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T10:10:49Z","timestamp":1790935849467,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1966,10,1]],"date-time":"1966-10-01T00:00:00Z","timestamp":-102643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1966,10]]},"abstract":"<jats:p>1. A technique for the radioautographic identification, localization, and study of the turnover of cellular depots of 5\u2010hydroxytryptamine (5\u2010HT) has been evaluated. A light microscopic survey was made of the uptake and turnover of 5\u2010HT, synthesized <jats:italic>in vivo<\/jats:italic> from its administered tritium\u2010labelled precursor, 5\u2010hydroxytryptophan (5\u2010HTP).<\/jats:p><jats:p>2. 5\u2010HT was taken up rapidly and retained for long periods of time by adrenal medullary chromaffin cells, gastric enterochromaffin cells, blood platelets, thyroid parafollicular cells, beta cells of pancreatic islets, mast cells, and septal cells of the lung. Reticulo\u2010endothelial cells of liver and spleen took up radioactive 5\u2010HT more slowly but also retained it for days.<\/jats:p><jats:p>3. Specific uptake, and a rapid turnover of radioactive 5\u2010HT, was found in pancreatic exocrine cells, neurones of the superior cervical ganglion, terminal axons of gastro\u2010intestinal myenteric plexus, carotid body cells, and pinealocytes.<\/jats:p><jats:p>4. A supranuclear localization of radioactive 5\u2010HT in renal proximal tubular cells may represent tubular secretion of 5\u2010HT.<\/jats:p><jats:p>5. The localization of 5\u2010HT by radioautography generally corresponds to that found by formaldehyde\u2010induced fluorescence. It makes possible the study of 5\u2010HT turnover and is capable of greater resolution than the fluorescence technique. Freeze\u2010drying may also be avoided.<\/jats:p>","DOI":"10.1113\/jphysiol.1966.sp008047","type":"journal-article","created":{"date-parts":[[2014,12,20]],"date-time":"2014-12-20T02:19:20Z","timestamp":1419041960000},"page":"477-492","source":"Crossref","is-referenced-by-count":148,"title":["Location of sites of 5\u2010hydroxytryptamine storage and metabolism by radioautography"],"prefix":"10.1113","volume":"186","author":[{"given":"M. D.","family":"Gershon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. L.","family":"Ross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1966,10]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1966.sp008047","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1966.sp008047","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T11:40:51Z","timestamp":1699443651000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1966.sp008047"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1966,10]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1966,10]]}},"alternative-id":["10.1113\/jphysiol.1966.sp008047"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1966.sp008047","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1966,10]]}}}