{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:32:14Z","timestamp":1771626734691,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1967,11,1]],"date-time":"1967-11-01T00:00:00Z","timestamp":-68428800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1967,11]]},"abstract":"<jats:p>1. 5\u2010Hydroxytryptamine (5\u2010HT) and various 5\u2010HT antagonists have been applied micro\u2010electrophoretically from multibarrelled micropipettes into the environment of single neurones in the post\u2010sigmoid and suprasylvian gyri of the cat cerebral cortex.<\/jats:p><jats:p>2. In unanaesthetized animals (enc\u00e9phale isol\u00e9) a high proportion of neurones (30%) were excited by 5\u2010HT. This excitation usually had a rapid onset and was seen both in spontaneously active neurones and in otherwise quiescent neurones in which firing was induced by \n<jats:sc>L<\/jats:sc>\u2010glutamate. Some neurones were so sensitive that the uncontrolled diffusion from micropipettes was sufficient to excite them. More cells were excited by 5\u2010HT applied as a cation from solutions of the bimaleate salt than when solutions of the creatinine sulphate salt were used.<\/jats:p><jats:p>3. In a high proportion of cells (33%) spontaneous firing or amino acid excitation was depressed by 5\u2010HT.<\/jats:p><jats:p>4. A mixed effect was seen in a small proportion (6%) of the cells tested; usually 5\u2010HT caused an excitation initially which was followed by a depression. In other cells, desensitization occurred, and the excitatory effect of 5\u2010HT was diminished or lost.<\/jats:p><jats:p>5. When glutamate was used to excite otherwise quiescent cells, there was a significant increase in the number of cells excited by 5\u2010HT and a significant decrease in the number of cells unaffected compared with spontaneously active cells.<\/jats:p><jats:p>6. The micro\u2010electrophoretic application of \n<jats:sc>D<\/jats:sc>\u2010lysergic diethylamide (LSD 25), 2\u2010brom LSD (BOL 148), methysergide (UML 491), or 2\u2032\u2010 (3\u2010dimethylaminopropylthio)cinnamanilide (SQ 10643) temporarily prevented excitation by 5\u2010HT in half the cells tested. LSD and SQ 10643 were particularly potent in this respect. This antagonism of 5\u2010HT excitation could still be seen when excitation of the cell by \n<jats:sc>L<\/jats:sc>\u2010glutamate or acetylcholine (ACh) was unaffected.<\/jats:p><jats:p>7. The depression induced by 5\u2010HT was not prevented by the application of known 5\u2010HT antagonists in the majority of the cells tested (93%). In two cells, however, the depression was reversibly prevented by these antagonists.<\/jats:p><jats:p>8. Some cells tested with 5\u2010HT were also tested with ACh or (\u2014)\u2010noradrenaline. The response of a cell to ACh was not significantly related to its response to 5\u2010HT. The degree of correlation between the responses to noradrenaline and 5\u2010HT was large, but not statistically significant with the small number of cells studied.<\/jats:p><jats:p>9. The effects of 5\u2010HT on cells in animals anaesthetized with \u03b1\u2010chloralose did not differ significantly from its effects in unanaesthetized preparations. It is suggested that the use of this anaesthetic may prove a useful alternative to unanaesthetized preparations.<\/jats:p><jats:p>10. The systemic injection of small quantities of thiopentone sodium selectively and reversibly reduced the sensitivity of some units to excitation by 5\u2010HT at a time when the response to glutamate was unaffected. On other occasions, the 5\u2010HT excitation was unaffected, though the response to glutamate was reduced.<\/jats:p><jats:p>11. These results are discussed in relation to the possible nature of the 5\u2010HT receptors in the cerebral cortex, and the interfering effects of anaesthesia on the response of brain cells to potential transmitter substances.<\/jats:p>","DOI":"10.1113\/jphysiol.1967.sp008357","type":"journal-article","created":{"date-parts":[[2014,12,20]],"date-time":"2014-12-20T00:58:32Z","timestamp":1419037112000},"page":"269-294","source":"Crossref","is-referenced-by-count":231,"title":["Excitation and depression of cortical neurones by 5\u2010hydroxytryptamine"],"prefix":"10.1113","volume":"193","author":[{"given":"M. H. T.","family":"Roberts","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. W.","family":"Straughan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1967,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1967.sp008357","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1967.sp008357","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T19:59:29Z","timestamp":1699473569000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1967.sp008357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1967,11]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1967,11]]}},"alternative-id":["10.1113\/jphysiol.1967.sp008357"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1967.sp008357","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1967,11]]}}}