{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T03:44:54Z","timestamp":1771645494191,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1969,3,1]],"date-time":"1969-03-01T00:00:00Z","timestamp":-26438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1969,3]]},"abstract":"<jats:p>1. A total of nineteen ram and three goat testes have been perfused in isolation at 34\u201335\u00b0 C for 3\u00bd\u20109 hr with heparinized blood and an added 5\u2010HT antagonist (bromolysergic acid diethylamide) and their function compared with that of the normal ram testes <jats:italic>in vivo<\/jats:italic>.<\/jats:p><jats:p>2. The metabolism of the perfused testes and the testes <jats:italic>in vivo<\/jats:italic> was similar but blood flow through the perfused testes was two to three times normal.<\/jats:p><jats:p>3. Vasoconstriction was produced by adrenaline and noradrenaline (10\u201320 \u03bcg \n<jats:sc>I.A.<\/jats:sc>) and by electrical stimulation of nerves in the spermatic cord or of the lumbar sympathetic chain; these responses were abolished or reduced by phenoxybenzamine.<\/jats:p><jats:p>4. Flow of fluid from the rete testis continued only if ischaemia was reduced to a minimum and glucose concentration in the blood perfusing the testis was kept above about 25 mg\/100 ml.; the fluid secreted during perfusion was of normal composition.<\/jats:p><jats:p>5. The perfused testis showed no evidence of autoregulation and the flow of fluid was not affected by changes in perfusion pressure.<\/jats:p><jats:p>6. When the temperature of three testes was raised to 40\u00b0 C for 2 hr, metabolism increased but blood flow was unaltered; the flow of fluid and the concentration of spermatozoa decreased during heating.<\/jats:p><jats:p>7. The testes perfused at normal scrotal temperatures (34\u201335\u00b0 C) were histologically normal but some abnormalities were observed in the heated testes.<\/jats:p>","DOI":"10.1113\/jphysiol.1969.sp008747","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T11:54:30Z","timestamp":1418990070000},"page":"129-143","source":"Crossref","is-referenced-by-count":17,"title":["Metabolism, sperm and fluid production of the isolated perfused testis of the sheep and goat"],"prefix":"10.1113","volume":"201","author":[{"given":"J. L.","family":"Linzell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. P.","family":"Setchell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1969,3]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1969.sp008747","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1969.sp008747","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T16:43:15Z","timestamp":1699461795000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1969.sp008747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1969,3]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1969,3]]}},"alternative-id":["10.1113\/jphysiol.1969.sp008747"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1969.sp008747","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1969,3]]}}}