{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:29:14Z","timestamp":1790418554963,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1971,2,1]],"date-time":"1971-02-01T00:00:00Z","timestamp":34214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1971,2]]},"abstract":"<jats:p>1. Insulin deficiency induced by anti\u2010insulin serum or streptozotocin increased glucose absorption, as measured in everted sacs of rat upper ileum incubated for 30 min with oxygenated Krebs\u2014Henseleit bicarbonate medium.<\/jats:p><jats:p>2. Everted sacs prepared from the terminal ileum of insulin\u2010deficient rats were able to accumulate glucose against a concentration gradient (i.e. development of active glucose transport).<\/jats:p><jats:p>3. In experimental diabetes induced by streptozotocin, everted sacs of upper ileum showed increased 3\u2010methyl glucose active transport, and sacs of terminal ileum showed development of 3\u2010methyl glucose active transport.<\/jats:p><jats:p>4. Lactic acid formation during the absorption of both glucose and 3\u2010methyl glucose was increased approximately twofold in everted sacs of insulin\u2010deficient animals.<\/jats:p><jats:p>5. Insulin added at 100 mu.\/ml. to the incubating media of everted sacs prepared from insulin\u2010deficient rats did not result in a reduction of glucose absorption or reverse the other effects.<\/jats:p><jats:p>6. Fluoride (5 \u00d7 10<jats:sup>\u22123<\/jats:sup> \n<jats:sc>M<\/jats:sc>) added to the serosal and mucosal media of sacs of terminal ileum prepared from insulin\u2010deficient rats decreased [<jats:sup>14<\/jats:sup>C]CO<jats:sub>2<\/jats:sub> formation from [U\u2010<jats:sup>14<\/jats:sup>C]glucose and lactate formation during glucose absorption, but was unable to reverse the effect of insulin deficiency on glucose active transport.<\/jats:p><jats:p>7. The effects of insulin deficiency induced by streptozotocin were more striking than those induced by anti\u2010insulin serum.<\/jats:p><jats:p>8. Everted sacs prepared from rats starved for 3 days showed increased glucose active transport accompanied by diminished conversion of [U\u2010<jats:sup>14<\/jats:sup>C]glucose to [<jats:sup>14<\/jats:sup>C]CO<jats:sub>2<\/jats:sub>.<\/jats:p><jats:p>9. The possible role of hexokinase is discussed in relation to these findings.<\/jats:p>","DOI":"10.1113\/jphysiol.1971.sp009358","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T10:59:29Z","timestamp":1418986769000},"page":"819-838","source":"Crossref","is-referenced-by-count":34,"title":["The effect of insulin and insulin deficiency on the transport and metabolism of glucose by rat small intestine"],"prefix":"10.1113","volume":"212","author":[{"given":"H. J.","family":"Leese","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. R. L.","family":"Mansford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1971,2]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1971.sp009358","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1971.sp009358","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T17:24:09Z","timestamp":1699464249000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1971.sp009358"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,2]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1971,2]]}},"alternative-id":["10.1113\/jphysiol.1971.sp009358"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1971.sp009358","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,2]]}}}