{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T23:35:15Z","timestamp":1771630515040,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1971,7,1]],"date-time":"1971-07-01T00:00:00Z","timestamp":47174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1971,7]]},"abstract":"<jats:p>1. Field stimulation of desheathed preparations of guinea\u2010pig vas deferens, treated with a ganglion\u2010blocking agent, has revealed the presence of two tetrodotoxin\u2010susceptible components in the motor response, suggesting the existence of two sets of post\u2010ganglionic motor nerve fibres of different excitability: one set responding maximally to pulses of 0\u00b71\u20130\u00b74 msec; the other, to pulses of 2 msec. No distinction could be made pharmacologically between the two components.<\/jats:p><jats:p>2. Cooling potentiated that component in the twitch\u2010responses which was due to stimulation of the more excitable fibres.<\/jats:p><jats:p>3. The sensitivity of the longitudinal muscle to the motor action of noradrenaline was low and was subject to considerable animal variation. But normal responses to post\u2010ganglionic field stimulation were elicited in noradrenaline\u2010insensitive preparations, in which the twitches elicited by 5 pulses could not be matched with noradrenaline, even 100\u2013125 \u03bcg\/ml.<\/jats:p><jats:p>4. In some forty experiments, small doses of noradrenaline inhibited the twitch\u2010responses evoked by either set of motor fibres. This inhibition differed from that produced by isoprenaline in two respects. Firstly, propranolol did not antagonize the noradrenaline inhibition, thus excluding an action on \u03b2\u2010adrenoceptors; and secondly, noradrenaline did not depress contractions elicited by muscarine or by 5\u2010methylfurmethide.<\/jats:p><jats:p>5. Phenoxybenzamine, 10<jats:sup>\u22126<\/jats:sup> g\/ml., produced a thousandfold reduction in the sensitivity of the muscle to the motor action of noradrenaline, without any decrease in the height of the twitches elicited by 0\u00b71 or 1 msec pulses.<\/jats:p><jats:p>6. The twitch\u2010responses were not affected by combined \u03b1 + \u03b2 adrenoceptor blockade with phentolamine and propranolol.<\/jats:p><jats:p>7. Tyramine, amphetamine, tranylcypromine and prostaglandin E<jats:sub>2<\/jats:sub> inhibited the twitches but potentiated the contractile effect of noradrenaline.<\/jats:p><jats:p>8. The twitch\u2010responses and their inhibition by noradrenaline were present in preparations taken from reserpinized animals.<\/jats:p><jats:p>9. Although the twitch\u2010responses could be paralysed by bretylium or guanethidine, the foregoing results excluded adrenergic transmission at the motor endings. Cholinergic transmission was also excluded by negative findings with anticholinesterases, atropine, nicotine and (+)\u2010tubocurarine.<\/jats:p><jats:p>10. Motor transmission by histamine, 5\u2010hydroxytryptamine, \u03b3\u2010aminobutyric acid or ATP was also excluded.<\/jats:p>","DOI":"10.1113\/jphysiol.1971.sp009530","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T11:12:41Z","timestamp":1418987561000},"page":"359-389","source":"Crossref","is-referenced-by-count":212,"title":["Evidence against adrenergic motor transmission in the guinea\u2010pig vas deferens"],"prefix":"10.1113","volume":"216","author":[{"given":"N.","family":"Ambache","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Aboo","family":"Zar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1971,7]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1971.sp009530","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1971.sp009530","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T16:42:52Z","timestamp":1699461772000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1971.sp009530"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,7]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1971,7]]}},"alternative-id":["10.1113\/jphysiol.1971.sp009530"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1971.sp009530","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,7]]}}}