{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T21:56:37Z","timestamp":1705010197215},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1971,11,1]],"date-time":"1971-11-01T00:00:00Z","timestamp":57801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1971,11]]},"abstract":"<jats:p>1. The Ca movements in normal and \u2018ghost\u2019 L cells have been examined; all measurements were made using <jats:sup>45<\/jats:sup>Ca.<\/jats:p><jats:p>2. Normal cells have a Ca concentration of about 1  m\u2010mole\/l. of cell volume, and exchange Ca in a complex way but with great rapidity; the time taken for the initial Ca<jats:sup>*<\/jats:sup> content to fall to half was less than 2 min.<\/jats:p><jats:p>3. Poisoning normal cells with DNP 10<jats:sup>\u22123<\/jats:sup> \n<jats:sc>M<\/jats:sc> + IAA 10<jats:sup>\u22124<\/jats:sup> \n<jats:sc>M<\/jats:sc> causes a marked reduction in the Ca efflux, no change in Ca influx and an increase in total Ca.<\/jats:p><jats:p>4. Variation in internal or external Na concentration does not alter the Ca fluxes or concentrations. Application of cyanide or ouabain and alteration of external K concentration had no effect on the Ca fluxes.<\/jats:p><jats:p>5. The sulphydryl reagents, ethacrynic acid and \n<jats:sc>N<\/jats:sc>\u2010ethylmaleimide (NEM), have a rapid and marked effect on reducing the Ca efflux.<\/jats:p><jats:p>6. L cell ghosts previously poisoned with DNP+IAA have a low Ca efflux. When ATP or CTP is incorporated into such cells the Ca efflux becomes normal.<\/jats:p><jats:p>7. An extra amount of phosphate is produced by L cell ghosts when pumping Ca. This is equivalent to the splitting of 1\u00b78 moles of ATP per mole of Ca pumped.<\/jats:p><jats:p>8. It is concluded that L cells have a Ca pump driven by ATP, and that Na has no effect on Ca movements in these cells.<\/jats:p>","DOI":"10.1113\/jphysiol.1971.sp009640","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T11:13:51Z","timestamp":1418987631000},"page":"691-708","source":"Crossref","is-referenced-by-count":28,"title":["Effect of Na, metabolic inhibitors and ATP on Ca movements in L cells"],"prefix":"10.1113","volume":"218","author":[{"given":"J. F.","family":"Lamb","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Lindsay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1971,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1971.sp009640","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1971.sp009640","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T15:03:02Z","timestamp":1699455782000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1971.sp009640"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,11]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1971,11]]}},"alternative-id":["10.1113\/jphysiol.1971.sp009640"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1971.sp009640","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,11]]}}}