{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,9]],"date-time":"2023-11-09T00:37:53Z","timestamp":1699490273929},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1972,12,1]],"date-time":"1972-12-01T00:00:00Z","timestamp":92016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1972,12]]},"abstract":"<jats:p>1. Vascular responses and noradrenaline overflows have been studied in the isolated blood\u2010perfused cat spleen in response to electrical stimulation of the splenic nerve with trains of 200 supramaximal pulses at a frequency of 10 Hz given at 10 min intervals.<\/jats:p><jats:p>2. In the absence of blocking agents the spleen gave well\u2010defined responses and a mean maximum overflow of 436 \u00b1 96 pg noradrenaline per stimulus (<jats:italic>n<\/jats:italic> = 4) at the third train of stimuli.<\/jats:p><jats:p>3. Cocaine (2 \u00d7 10<jats:sup>\u22125<\/jats:sup> \n<jats:sc>M<\/jats:sc>) increased the response nearly threefold and raised the overflow to 840 \u00b1 131 pg\/stim (<jats:italic>n<\/jats:italic> = 4). At later stimulations the responses remained stable, but the overflows rose progressively to 1076 \u00b1 51 pg\/stim after five trains of stimuli.<\/jats:p><jats:p>4. Normetanephrine (10<jats:sup>\u22124<\/jats:sup> \n<jats:sc>M<\/jats:sc>) had no significant effect on response, but elevated the overflow to a mean maximum of 868 \u00b1 169 pg\/stim (<jats:italic>n<\/jats:italic> = 4).<\/jats:p><jats:p>5. Cocaine (2 \u00d7 10<jats:sup>\u22125<\/jats:sup> \n<jats:sc>M<\/jats:sc>) and normetanephrine (10<jats:sup>\u22124<\/jats:sup> \n<jats:sc>M<\/jats:sc>) given together increased the response by a factor of 6\u00b75 and raised the overflow to 1258 \u00b1 247 pg\/stim (<jats:italic>n<\/jats:italic> = 4).<\/jats:p><jats:p>6. Hydergine (approx. 10<jats:sup>\u22125<\/jats:sup> \n<jats:sc>M<\/jats:sc>) almost completely abolished the response and raised the overflow to 859 \u00b1 173 pg\/stim (<jats:italic>n<\/jats:italic> = 4).<\/jats:p><jats:p>7. Cocaine (2 \u00d7 10<jats:sup>\u22125<\/jats:sup> \n<jats:sc>M<\/jats:sc>), normetanephrine (10<jats:sup>\u22124<\/jats:sup> \n<jats:sc>M<\/jats:sc>) and hydergine (approx. 10<jats:sup>\u22125<\/jats:sup> \n<jats:sc>M<\/jats:sc>) given in combination abolished the response and raised the overflow to 4089 \u00b1 1148 pg\/stim (<jats:italic>n<\/jats:italic> = 4).<\/jats:p><jats:p>8. Phenoxybenzamine (10<jats:sup>\u22124<\/jats:sup> \n<jats:sc>M<\/jats:sc>) abolished the response and elevated the overflow to 4215 \u00b1 738 pg\/stim (<jats:italic>n<\/jats:italic> = 4).<\/jats:p><jats:p>9. These results are interpreted in terms of selective and combined block of \u2018uptake I\u2019, \u2018uptake II\u2019 and \u03b1\u2010adrenergic receptors. Facilitation of transmitter release by \u03b1\u2010blocking drugs, and the possible existence of an uptake process associated with the \u03b1\u2010receptors are discussed.<\/jats:p>","DOI":"10.1113\/jphysiol.1972.sp010052","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T10:53:34Z","timestamp":1418986414000},"page":"647-664","source":"Crossref","is-referenced-by-count":17,"title":["Vascular responses and noradrenaline overflows in the isolated blood\u2010perfused cat spleen: some effects of cocaine, normetanephrine and \u03b1\u2010blocking agents"],"prefix":"10.1113","volume":"227","author":[{"given":"Heather","family":"Cripps","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. P.","family":"Dearnaley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1972,12]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1972.sp010052","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1972.sp010052","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T21:25:40Z","timestamp":1699478740000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1972.sp010052"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,12]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1972,12]]}},"alternative-id":["10.1113\/jphysiol.1972.sp010052"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1972.sp010052","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,12]]}}}