{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T07:56:54Z","timestamp":1770969414403,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1973,6,1]],"date-time":"1973-06-01T00:00:00Z","timestamp":107740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1973,6]]},"abstract":"<jats:p>1. Intracellular recordings of membrane potentials have been made <jats:italic>in vitro<\/jats:italic> from the exocrine acinar cells of the mouse pancreas using glass micro\u2010electrodes.<\/jats:p><jats:p>2. The mean membrane potential of the acinar cells during superfusion with Krebs\u2010Henseleit solution was \u221239\u00b72 mV. Increasing [K]<jats:sub>o<\/jats:sub> tenfold decreased the membrane potential by 28 mV when [K]<jats:sub>o<\/jats:sub> was above 10 m\n<jats:sc>M<\/jats:sc>. This depolarization was not affected by atropine (1\u00b74 \u00d7 10<jats:sup>\u22126<\/jats:sup> \n<jats:sc>M<\/jats:sc>). Strophanthin\u2010G (10<jats:sup>\u22123<\/jats:sup> \n<jats:sc>M<\/jats:sc>) slowly depolarized the cells at about 10 mV hr<jats:sup>\u22121<\/jats:sup>.<\/jats:p><jats:p>3. Brief exposure to acetylcholine (ACh), 5\u00b75 \u00d7 10<jats:sup>\u22125<\/jats:sup> \n<jats:sc>M<\/jats:sc>, or pancreozymin resulted in a short lasting depolarization of the acinar cells. Atropine (1\u00b74 \u00d7 10<jats:sup>\u22126<\/jats:sup> \n<jats:sc>M<\/jats:sc>) blocked the depolarizing action of ACh but not that of pancreozymin. Adrenaline (5\u00b75 \u00d7 10<jats:sup>\u22125<\/jats:sup> \n<jats:sc>M<\/jats:sc>) or cyclic AMP (10<jats:sup>\u22123<\/jats:sup>\u201010<jats:sup>\u22124<\/jats:sup> \n<jats:sc>M<\/jats:sc>) did not influence the membrane potential.<\/jats:p><jats:p>4. The amplitude of the ACh\u2010induced depolarization was not dependent on the presence of CO<jats:sub>2<\/jats:sub>\/HCO<jats:sub>3<\/jats:sub> in the bathing fluid, but it was closely dependent on the extracellular Na concentration. However, ACh was still able to evoke a small depolarization even after prolonged exposure of the tissue to a Na\u2010free solution.<\/jats:p><jats:p>5. During exposure of the tissue to a Ca\u2010free solution the resting membrane potential was decreased and the ACh\u2010induced depolarization was significantly reduced. Some substances which are known in other tissues to inhibit membrane Ca<jats:sup>2+<\/jats:sup> currents, i.e. La<jats:sup>3+<\/jats:sup>, D\u2010600 and tetracaine, were able to reduce, but never abolish, the ACh\u2010induced depolarization.<\/jats:p><jats:p>6. These results suggest that the effect of ACh on the pancreatic acinar cell is to increase the permeability of the membrane to commonly occurring ions with a consequent Na\u2010influx and a small Ca\u2010influx.<\/jats:p>","DOI":"10.1113\/jphysiol.1973.sp010233","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T10:24:17Z","timestamp":1418984657000},"page":"283-295","source":"Crossref","is-referenced-by-count":56,"title":["Pancreatic acinar cells: ionic dependence of the membrane potential and acetylcholine\u2010induced depolarization"],"prefix":"10.1113","volume":"231","author":[{"given":"E. K.","family":"Matthews","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O. H.","family":"Petersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1973,6]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1973.sp010233","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1973.sp010233","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T13:07:42Z","timestamp":1699448862000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1973.sp010233"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1973,6]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1973,6]]}},"alternative-id":["10.1113\/jphysiol.1973.sp010233"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1973.sp010233","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1973,6]]}}}