{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T23:28:58Z","timestamp":1771630138544,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1974,10,1]],"date-time":"1974-10-01T00:00:00Z","timestamp":149817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1974,10]]},"abstract":"<jats:p>1. Na and K contents of isolated rat superior cervical ganglia were measured by flame photometry, and intracellular Na and K concentrations ([Na]<jats:sub>i<\/jats:sub> and [K]<jats:sub>i<\/jats:sub>) calculated using Li and <jats:sup>35<\/jats:sup>SO<jats:sub>4<\/jats:sub> to determine extracellular space (e.c.s.).<\/jats:p><jats:p>2. Resting concentrations after 1\u20102 hr incubation at 25\u00b0 C in normal Krebs solution were: [Na]<jats:sub>i<\/jats:sub>, 19\u00b78 \u00b1 0\u00b79 m\u2010mole (kg cell water)<jats:sup>\u22121<\/jats:sup>; [K]<jats:sub>i<\/jats:sub>, 192\u00b77 \u00b1 2\u00b78 m\u2010mole (kg cell water)<jats:sup>\u22121<\/jats:sup> (mean \u00b1 \n<jats:sc>S.E.<\/jats:sc> of mean of thirty\u2010five ganglia). Correction for losses during e.c.s. measurement gave 22 m\n<jats:sc>M<\/jats:sc> [Na]<jats:sub>i<\/jats:sub> and 207 m\n<jats:sc>M<\/jats:sc> [K]<jats:sub>i<\/jats:sub> as probable fresh concentrations.<\/jats:p><jats:p>3. Carbachol (180 \u03bc\n<jats:sc>M<\/jats:sc> for 4 min) increased [Na]<jats:sub>i<\/jats:sub> by 47\u00b78 \u00b1 2\u00b79 m\u2010mole (kg cell water)<jats:sup>\u22121<\/jats:sup> and decreased [K]<jats:sub>i<\/jats:sub> by 54\u00b76 \u00b1 4\u00b73 m\u2010mole (kg cell water)<jats:sup>\u22121<\/jats:sup>. Maximal exchange with carbachol or nicotine (at \u223c 1 m\n<jats:sc>M<\/jats:sc> for 4 min) amounted to 80\u2013100 m\u2010mole (kg cell water)<jats:sup>\u22121<\/jats:sup>. On washing with Krebs solution containing 2\u00b75 m\n<jats:sc>M<\/jats:sc> hexamethonium recovery of ionic concentrations occurred with a rate constant of 0\u00b73\u20130\u00b74 min<jats:sup>\u22121<\/jats:sup>.<\/jats:p><jats:p>4. Restitution of ganglionic Na and K after carbachol was inhibited by washing with K\u2010free solution, and slowed by ouabain (0\u00b714 m\n<jats:sc>M<\/jats:sc>), cyanide (2 m\n<jats:sc>M<\/jats:sc>) or cooling (<jats:italic>Q<\/jats:italic><jats:sub>10<\/jats:sub> 2\u00b77 between 17 and 27\u00b0 C).<\/jats:p><jats:p>5. Equilibrium potentials for Na and K (<jats:italic>E<\/jats:italic><jats:sub>Na<\/jats:sub>, <jats:italic>E<\/jats:italic><jats:sub>K<\/jats:sub>) at rest were calculated to be +49 and \u221288 mV. At a membrane potential (<jats:italic>E<\/jats:italic><jats:sub>m<\/jats:sub>) of \u221270 m\n<jats:sc>V<\/jats:sc>, the permeability ratio <jats:italic>P<\/jats:italic><jats:sub>Na<\/jats:sub>:<jats:italic>P<\/jats:italic><jats:sub>K<\/jats:sub> was calculated at 0\u00b704:1 (assuming <jats:italic>P<\/jats:italic><jats:sub>Cl<\/jats:sub>:<jats:italic>P<\/jats:italic><jats:sub>K<\/jats:sub> &lt; 0\u00b71).<\/jats:p>","DOI":"10.1113\/jphysiol.1974.sp010709","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T10:12:13Z","timestamp":1418983933000},"page":"307-319","source":"Crossref","is-referenced-by-count":38,"title":["Changes of intracellular sodium and potassium ion concentrations in isolated rat superior cervical ganglia induced by depolarizing agents"],"prefix":"10.1113","volume":"242","author":[{"given":"D. A.","family":"Brown","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. N.","family":"Scholfield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1974,10]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1974.sp010709","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1974.sp010709","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T18:16:06Z","timestamp":1699208166000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1974.sp010709"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,10]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1974,10]]}},"alternative-id":["10.1113\/jphysiol.1974.sp010709"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1974.sp010709","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,10]]}}}