{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T18:40:21Z","timestamp":1699209621011},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1974,10,1]],"date-time":"1974-10-01T00:00:00Z","timestamp":149817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1974,10]]},"abstract":"1. Isolated rat superior cervical ganglia were incubated in Krebs solution containing 24<\/jats:sup>Na and carbachol for 4 min at 25\u00b0 C. They were then washed at 3\u00b0 C for 15 min to remove extracellular 24<\/jats:sup>Na and the efflux of residual intracellular 24<\/jats:sup>Na stimulated by warming to 25\u00b0 C.<\/jats:p>2. During the 15 min wash at 3\u00b0 C desaturation curves became exponential with a rate constant of 0\u00b7012 \u00b1 0\u00b7001 min\u22121<\/jats:sup> (n<\/jats:italic> = 24). This was assumed to represent loss of intracellular 24<\/jats:sup>Na, and initial uptake of 24<\/jats:sup>Na was calculated therefrom by back\u2010extrapolation to zero wash\u2010time. After 4 min in 24<\/jats:sup>Na + 180 \u03bc\nM<\/jats:sc> carbachol intracellular [24<\/jats:sup>Na] so calculated was 61\u00b76 \u00b1 3\u00b71 m\nM<\/jats:sc> (n<\/jats:italic> = 18), representing 83% labelling of intracellular Na. In the absence of carbachol intracellular [24<\/jats:sup>Na] was 10\u00b70 \u00b1 0\u00b75 m\nM<\/jats:sc>, representing 49% labelling. Extracellular Na was labelled by > 90% after 4 min in 24<\/jats:sup>Na. The apparent rate constant for washout of extracellular 24<\/jats:sup>Na was 0\u00b76 min\u22121<\/jats:sup> at 3\u00b0 C and 0\u00b795 min\u22121<\/jats:sup> at 25\u00b0 C.<\/jats:p>3. The loss of the residual intracellular 24<\/jats:sup>Na during temperature stimulation was interpreted quantitatively in terms of an exponential decline of the bulk of intracellular 24<\/jats:sup>Na with an extrusion rate constant of 0\u00b739 \u00b1 0\u00b71 min\u22121<\/jats:sup> (n<\/jats:italic> = 18), efflux being delayed by passage through the extracellular space with an effective rate constant of 0\u00b78\u20131\u00b72 min\u22121<\/jats:sup>.<\/jats:p>4. The peak rate constant (k<\/jats:italic>C<\/jats:sup>) for the desaturation curve at 25\u00b0 C was 0\u00b735 \u00b1 0\u00b701 min\u22121<\/jats:sup>. An Arrhenius plot of log k<\/jats:italic>C<\/jats:sup>\/T<\/jats:italic>\u00b0 K<\/jats:italic>\u22121<\/jats:sup> yielded a two\u2010stage linear regression with a transition at 20\u00b0 C. Activation energies of 8 and 31 kcal. mole\u22121<\/jats:sup> were calculated above and below this transition respectively.<\/jats:p>5. Omission of K from the 25\u00b0 C temperature\u2010stimulating solution reduced k<\/jats:italic>C<\/jats:sup> by 62%. The K\u2010sensitive component of extrusion rate constant was a hyperbolic function of [K]e<\/jats:sub> with half\u2010saturation at 5\u00b76 m\nM<\/jats:sc>\u2010[K]e<\/jats:sub> and maximum k<\/jats:italic>C<\/jats:sup> of 0\u00b758 min\u22121<\/jats:sup>.<\/jats:p>6. Cyanide (2 m\nM<\/jats:sc>), 2,4\u2010dinitrophenol (1 m\nM<\/jats:sc>) and ouabain (1\u00b74 m\nM<\/jats:sc>) reduced k<\/jats:italic>C<\/jats:sup> by 50\u201390%. The half\u2010maximally inhibiting concentration of ouabain was about 60 \u03bc\nM<\/jats:sc>.<\/jats:p>7. Substitution of sucrose, Li or choline for external Na did not reduce the extrusion rate of 24<\/jats:sup>Na in either 6 m\nM<\/jats:sc>\u2010[K]e<\/jats:sub> or 0 m\nM<\/jats:sc>\u2010[K]e<\/jats:sub>. Li stimulated 24<\/jats:sup>Na extrusion in Na\u2010free, K\u2010free solution.<\/jats:p>8. The properties of the ganglionic Na pump deduced from rates of temperature\u2010stimulated 24<\/jats:sup>Na extrusion accord with the view that the ganglion hyperpolarization observed after Na loading by exposure to nicotinic depolarizing agents results from electrogenic Na extrusion. A comparable hyperpolarization is observed after temperature stimulation following Na loading.<\/jats:p>","DOI":"10.1113\/jphysiol.1974.sp010710","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T10:12:13Z","timestamp":1418983933000},"page":"321-351","source":"Crossref","is-referenced-by-count":19,"title":["Movements of labelled sodium ions in isolated rat superior cervical ganglia"],"prefix":"10.1113","volume":"242","author":[{"given":"D. A.","family":"Brown","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. N.","family":"Scholfield","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1974,10]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1974.sp010710","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1974.sp010710","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T18:16:08Z","timestamp":1699208168000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1974.sp010710"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1974,10]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1974,10]]}},"alternative-id":["10.1113\/jphysiol.1974.sp010710"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1974.sp010710","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1974,10]]}}}