{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T07:56:54Z","timestamp":1770969414366,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1975,1,1]],"date-time":"1975-01-01T00:00:00Z","timestamp":157766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1975,1]]},"abstract":"<jats:p>1. Intracellular recordings of membrane potential, input resistance and time constant have been made in vitro from the exocrine acinar cells of the mouse pancreas using glass micro\u2010electrodes. The acinar cells were stimulated by acetylcholine (ACh). In some cases ACh was simply directly added to the tissue superfusion bath, in other experiments ACh was applied locally to pancreatic acini by micro\u2010iontophoresis. 2. Current\u2010voltage relations were investigated by injecting rectangular de\u2010 or hyperpolarizing current pulses through the recording micro\u2010electrode. Within a relatively wide range (\u221220 to \u221270 mV) there was a linear relation between injected current and change in membrane potential. The slope of such linear curves corresponded to an input resistance of about 3\u20108 M omega. The membrane time constant was about 5\u201310 msec. 3. ACh depolarized the cell membrane and caused a marked reduction of input resistance and time constant. The minimum latency of the ACh\u2010induced depolarization (microiontophoretic application) was 100\u2013300 msec. Maximal depolarization was about 20 mV. The effect of this local ACh application was abolished by atropine (1\u20134 \u00d7 10\u20136 M). The blocking effect of atropine was fully reversible. 4. Stimulating with ACh during the passage of large depolarizing current pulses made it possible simultaneously to observe the effect of ACh at two different levels of resting potential (RP). At the spontaneous RP of about minus 40 mV ACh evoked a depolarization of usual magnitude (15\u201320 mV) while at the artificially displaced level of about \u221210 mV a small hyperpolarization (about 5 mV) was observed. It therefore appears that the reversal potential of the transmitter equilibrium potential is about \u221220 mV. 5. Replacement of the superfusion fluid C1 by sulphate or methylsulphate caused an initial short\u2010lasting depolarization, thereafter the normal resting potential was reassumed...<\/jats:p>","DOI":"10.1113\/jphysiol.1975.sp010807","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T09:13:20Z","timestamp":1418980400000},"page":"431-465","source":"Crossref","is-referenced-by-count":52,"title":["Pancreatic acinar cells: ionic dependence of acetylcholine\u2010induced membrane potential and resistance change."],"prefix":"10.1113","volume":"244","author":[{"given":"A","family":"Nishiyama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O H","family":"Petersen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1975,1]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1975.sp010807","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1975.sp010807","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T04:00:47Z","timestamp":1699156847000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1975.sp010807"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1975,1]]}},"alternative-id":["10.1113\/jphysiol.1975.sp010807"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1975.sp010807","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1975,1]]}}}