{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:24:43Z","timestamp":1761017083623},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1976,3,1]],"date-time":"1976-03-01T00:00:00Z","timestamp":194486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1976,3]]},"abstract":"<jats:p>1. While much is now known about the Na\u2010K\u2010ATPase and the posttetanic hyperpolarization of nervous tissue, they have yet to be studied together in the same preparation. 2. The post\u2010tetanic hyperpolarization was studied in desheathed garfish olfactory nerve. The rate constant of decay of the post\u2010tetanic hyperpolarization was determined by monitoring difference potentials after stimulation at 1\/sec for 2\u20103 min. 3. In membrane fractions prepared from these nerves, the ouabain\u2010sensitive ATPase activity (Na\u2010K\u2010ATPase) was determined by spectrophotometric measurements. 4. Both the post\u2010tetanic hyperpolarization and the Na\u2010K\u2010ATPase showed a similar sigmoidal dependence on K+ concentration. The sequence of cation specificities measured at the K\u2010site of the enzyme was the same as that determined by post\u2010tetanic hyperpolarization measurements in whole nerve. 5. The rate constants of the enzyme showed a dependence on Na+ concentration that paralleled the way in which the post\u2010tetanic hyperpolarization rate constants varied as a function of the number of impulses. When Na+ was completely replaced by Li+, neither enzyme activity nor post\u2010tetanic hyperpolarization could be measured. 6. The pH optimum for enzyme activity was between pH 7\u20100 and 7\u20108, while the optimal pH for post\u2010tetanic hyperpolarization was above pH 8\u20100. 7. Metabolite levels in preparations of this nerve studied in vitro correspond to levels found in vivo. 8. High energy phosphate levels were measured fluorometrically in extracts of nerve samples that had been stimulated in air at 1\/sec for various intervals. 9. During the first 2 min of stimulation, there was a significant accumulation of inorganic phosphate, and the ATP\/ADP.Pi ratio dropped appreciably. 10. The accumulation of ATPase products was commensurate with the approach of post\u2010tetanic hyperpolarization rate constants to their maximum level. This provides direct evidence for an ATPase functioning in active Na+ transport in nerve. 11. The garfish Na\u2010K\u2010ATPase is sensitive to the ATP\/ADP ratio of the incubating medium, but is relatively insensitive to orthophosphate, Pi. The fall in post\u2010tetanic hyperpolarization rate constants observed with continued nerve stimulation may have been partially due to the falling ATP\/ADP ratio measured in nerve under similar conditions.<\/jats:p>","DOI":"10.1113\/jphysiol.1976.sp011310","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T09:01:43Z","timestamp":1418979703000},"page":"41-60","source":"Crossref","is-referenced-by-count":13,"title":["Post\u2010tetanic hyperpolarization, sodium\u2010potassium\u2010activated adenosine triphosphatase and high energy phosphate levels in garfish olfactory nerve."],"prefix":"10.1113","volume":"256","author":[{"given":"D B","family":"McDougal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L A","family":"Osborn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1976,3]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1976.sp011310","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1976.sp011310","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T06:24:23Z","timestamp":1699251863000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1976.sp011310"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,3]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1976,3]]}},"alternative-id":["10.1113\/jphysiol.1976.sp011310"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1976.sp011310","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1976,3]]}}}