{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:07:00Z","timestamp":1762067220249},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1978,9,1]],"date-time":"1978-09-01T00:00:00Z","timestamp":273456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1978,9]]},"abstract":"<jats:p>1. 45Ca efflux and tissue Ca content were examined in goldfish ventricles under conditions known to affect cellular Ca movements. 2. EGTA or Ca\u2010EGTA was added to the washout solutions in sufficient concentration (10 mM) to avoid retardation of the apparent tissue 45Ca efflux by extracellular 45Ca binding or backflux. 3. After a variable initial increase, the cellular Ca content usually stabilizes within 60 min when ventricles are immersed in Li\u2010 or K\u2010substituted saline containing 1.8 mM Ca0 (under these conditions the internal Ca2+ concentration is below 10(\u20105) M). 4. 45Ca efflux is maximally activated by external concentrations of Ca2+ as low as 10(\u20106) M, in both Na\u2010containing and Na\u2010free saline. 5. 45Ca efflux decreases in Na\u2010free solutions. It is reactivated by Na\u2010saline. The effect of different external Na concentration on 45Ca efflux is comparable at external Ca2+ concentrations between 10(\u20106) M and 2 x 10(\u20103) M. 6. Reactivation of Ca efflux after Na0 readmission is inhibited by metabolic poisoning, or in the presence of 10 mM\u2010caffeine. Loading with 45Ca at very low external Ca2+ concentration prevents the inhibition of Ca efflux in Na\u2010free medium. 7. Caffeine (10 mM) produces contractions of about equla size when K\u2010depolarized preparations are immersed in either Na\u2010 or Li\u2010saline. At the same time there is a similar increase in 45Ca efflux in absence of Na0 and in its presence. 8. In the virtual absence of Ca2+0 (10(\u20105) M\u2010Ca, 10(\u20102) M\u2010EGTA) and Na+0, the residual 45Ca efflux is reversibly inhibited by cyanide (2 mM). 9. The results are roughly compatible with the general concept of ATP\u2010dependent Na\u2010Ca exchange in internal Ca2+ homeostasis. However, this hypothesis should probably be modified to account for the fact that under physiological concentrations Na+0 and Ca2+0 do not compete for activating 45Ca efflux. Metabolic products may be involved in Na0\u2010 and Ca0\u2010dependent Ca efflux. It is therefore not excluded that a Na\u2010independent active mechanism co\u2010operates with Na\u2010Ca exchange in Ca extrusion.<\/jats:p>","DOI":"10.1113\/jphysiol.1978.sp012462","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:52:00Z","timestamp":1418975520000},"page":"263-283","source":"Crossref","is-referenced-by-count":16,"title":["The effect of sodium, calcium and metabolic inhibitors on calcium efflux from goldfish heart ventricles."],"prefix":"10.1113","volume":"282","author":[{"given":"P","family":"Busselen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"van Kerkhove","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1978,9]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1978.sp012462","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1978.sp012462","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T10:50:21Z","timestamp":1699181421000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1978.sp012462"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1978,9]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1978,9]]}},"alternative-id":["10.1113\/jphysiol.1978.sp012462"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1978.sp012462","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1978,9]]}}}