{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T17:40:30Z","timestamp":1699206030810},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1980,2,1]],"date-time":"1980-02-01T00:00:00Z","timestamp":318211200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1980,2]]},"abstract":"<jats:p>1. Effects of adenosine and the adenosine nucleotides (ATP, ADP, AMP and CoA) on the guinea\u2010pig taenia caecum were studied by measuring simultaneously the changes in membrane potential and in contractility induced by these agents using the sucrose\u2010gap technique.<\/jats:p><jats:p>2. In the presence of carbachol (5 \u00d7 10<jats:sup>\u22128<\/jats:sup> \n<jats:sc>M<\/jats:sc>) the hyperpolarization and the relaxation induced by these agents was found to be closely correlated. Their different potencies suggested an action on the P<jats:sub>2<\/jats:sub> type purinoceptor: ATP \u2265 ADP \u2265 AMP &gt; CoA &gt; AD.<\/jats:p><jats:p>3. The transmitter released endogenously in the presence of carbachol (5 \u00d7 10<jats:sup>\u22128<\/jats:sup> \n<jats:sc>M<\/jats:sc>) by the non\u2010adrenergic inhibitory (n.a.i.) nerves after half\u2010maximal stimulation induced an inhibitory junction potential (i.j.p.) which transiently counteracted the carbachol\u2010induced depolarization. This i.j.p. was mimicked by ATP, ADP, and AMP applied exogenously in concentrations of about 3 \u00d7 10<jats:sup>\u22125<\/jats:sup> \n<jats:sc>M<\/jats:sc>, by CoA (3 \u00d7 10<jats:sup>\u22124<\/jats:sup> \n<jats:sc>M<\/jats:sc>) and by adenosine (3 \u00d7 10<jats:sup>\u22123<\/jats:sup> \n<jats:sc>M<\/jats:sc>).<\/jats:p><jats:p>4. The results presented are in agreement with the hypothesis that the transmitter substance released by the n.a.i. nerves is a purine nucleotide, which in the guinea\u2010pig taenia caecum affects the smooth muscle cell membrane via the <jats:italic>P<\/jats:italic><jats:sub>2<\/jats:sub>\u2010purinoceptor. Of the putative transmitters studied, ATP, ADP and AMP seem the most likely.<\/jats:p>","DOI":"10.1113\/jphysiol.1980.sp013111","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:24:23Z","timestamp":1418973863000},"page":"75-83","source":"Crossref","is-referenced-by-count":14,"title":["A comparison of effects evoked in guinea\u2010pig taenia caecum by purine nucleotides and by \u2018purinergic\u2019 nerve stimulation"],"prefix":"10.1113","volume":"299","author":[{"given":"L. P.","family":"Jager","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. A. M.","family":"Schevers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1980,2]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1980.sp013111","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1980.sp013111","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T17:07:30Z","timestamp":1699204050000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1980.sp013111"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1980,2]]}},"alternative-id":["10.1113\/jphysiol.1980.sp013111"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1980.sp013111","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,2]]}}}