{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T01:36:19Z","timestamp":1759541779600},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1980,5,1]],"date-time":"1980-05-01T00:00:00Z","timestamp":325987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1980,5]]},"abstract":"<jats:p>1. The aim of the present work was to study the role of Na+, Ca2+ and cyclic nucleotides in stimulus\u2010secretion coupling in rat pancreatic acinar cells utilizing Ca2+ specific ionophore A23187 as well as ionophores X\u2010537A and BrX\u2010537A which transport both monovalent and divalent cations across biological membranes. 2. X\u2010537A, BrX\u2010537A and A23187 increased amylase secretion by a direct energy dependent action without damaging the cells. Antimycin\u2010A and dinitrophenol increased the spontaneous release of amylase. 3. In the absence of extracellular Ca2+, the secretory response to X\u2010537A and BrX\u2010537A was reduced by 57 and 50% respectively whereas A23187 was without effect. In the absence of extracellular Na+, both basal and ionophore stimulated secretion of amylase were increased. Replacement of Na+ by Li+ or Tris+ in Ca2+\u2010free medium abolished the increase in amylase secretion. 4. X\u2010537A increased bidirectional flux of 45Ca2+. Efflux of 45Ca2+ persisted in Ca2+\u2010free media. In Tris\u2010Ringer and Li\u2010Ringer, basal 45Ca2+ influx and amylase secretion were increased. Moreover X\u2010537A enhanced both 45Ca2+ efflux and amylase secretion. This effect was abolished in media containing no Na+ and Ca2+ suggesting that Na+ concentration gradient across the membrane is critical for digestive enzyme secretion. 5. Theophylline increased basal concentration of cyclic\u2010GMP at 1, 2, 5, 15 and 30 min. X\u2010537A stimulated cyclic\u2010GMP synthesis in presence of theophylline with a peak effect observed at 15 min with X\u2010537A. Incubation of pancreatic fragments in Ca2+\u2010free media containing EGTA (5 x 10(\u20105) M) decreased basal and X\u2010537A stimulated synthesis of cyclic\u2010GMP. 6. From these data it is concluded that Na+ plays a direct role in Ca2+ mobilization and exocytosis. Like other tissues, cyclic\u2010GMP synthesis is dependent on extracellular Ca2+ and does not seem to play a role in stimulus\u2010secretion coupling.<\/jats:p>","DOI":"10.1113\/jphysiol.1980.sp013226","type":"journal-article","created":{"date-parts":[[2015,12,16]],"date-time":"2015-12-16T23:18:11Z","timestamp":1450307891000},"page":"1-17","source":"Crossref","is-referenced-by-count":1,"title":["Stimulus\u2010secretion coupling in rat pancreas: role of sodium, calcium and cyclic nucleotides studied by X\u2010537A and BrX\u2010537A."],"prefix":"10.1113","volume":"302","author":[{"given":"M","family":"Singh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1980,5]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1980.sp013226","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1980.sp013226","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1980.sp013226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T19:28:15Z","timestamp":1699212495000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1980.sp013226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,5]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1980,5]]}},"alternative-id":["10.1113\/jphysiol.1980.sp013226"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1980.sp013226","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,5]]}}}