{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:17:30Z","timestamp":1761059850187},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1980,7,1]],"date-time":"1980-07-01T00:00:00Z","timestamp":331257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1980,7]]},"abstract":"<jats:p>1. ACh\u2010induced secretion from the main excretory duct, as well as ACh\u2010induced hyperpolarizations and resting membrane potentials of superficial, probably acinar, cells were recorded from the rabbit lacrimal gland perfused <jats:italic>in vivo<\/jats:italic> with either control or test solutions.<\/jats:p><jats:p>2. During perfusion with test solution containing a Ca antagonist, verapamil (10<jats:sup>\u22125<\/jats:sup> to 10<jats:sup>\u22123<\/jats:sup> \n<jats:sc>M<\/jats:sc>), or a Ca chelator EGTA (10<jats:sup>\u22124<\/jats:sup> or 10<jats:sup>\u22123<\/jats:sup> \n<jats:sc>M<\/jats:sc>), ACh\u2010induced secretion was only 30% of the control value, whereas the magnitude of the ACh\u2010induced hyperpolarization was unchanged.<\/jats:p><jats:p>3. With 10<jats:sup>\u22125<\/jats:sup> \n<jats:sc>M<\/jats:sc>\u2010verapamil, but not with 10<jats:sup>\u22123<\/jats:sup> \n<jats:sc>M<\/jats:sc>\u2010verapamil or EGTA\u2010containing solutions, the inhibition of ACh\u2010induced flow was completely reversed either by replacing the test with control solution or by increasing the Ca concentration of the test solution twofold.<\/jats:p><jats:p>4. In addition, EGTA (10<jats:sup>\u22124<\/jats:sup> \n<jats:sc>M<\/jats:sc>) solution containing the normal extracellular Ca<jats:sup>2+<\/jats:sup> concentration inhibited secretion by 30%.<\/jats:p><jats:p>5. During perfusion with verapamil or EGTA test solutions the mean resting membrane potential of superficial, probably acinar, cells was depolarized by 15%.<\/jats:p><jats:p>6. It is concluded that although extracellular Ca is required for the major portion of ACh\u2010induced secretion, the magnitude of the ACh\u2010induced hyperpolarization of cells, which are most likely acinar, is independent of extracellular Ca.<\/jats:p>","DOI":"10.1113\/jphysiol.1980.sp013331","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:21:11Z","timestamp":1418973671000},"page":"397-403","source":"Crossref","is-referenced-by-count":15,"title":["Effect of calcium antagonism or chelation on rabbit lacrimal gland secretion and membrane potentials"],"prefix":"10.1113","volume":"304","author":[{"given":"Stella Y.","family":"Botelho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darlene A.","family":"Dartt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1980,7]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1980.sp013331","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1980.sp013331","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T09:23:47Z","timestamp":1699176227000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1980.sp013331"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1980,7]]}},"alternative-id":["10.1113\/jphysiol.1980.sp013331"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1980.sp013331","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,7]]}}}