{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T22:24:31Z","timestamp":1771626271064,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1982,7,1]],"date-time":"1982-07-01T00:00:00Z","timestamp":394329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1982,7]]},"abstract":"1. Passive electrical properties of sheep cardiac Purkinje fibres were assessed by performing linear cable analysis. In a separate set of experiments pHi<\/jats:sub> was monitored using recessed\u2010tip pH\u2010sensitive micro\u2010electrodes.<\/jats:p>2. In Tris\u2010buffered Tyrode solution (nominally CO2<\/jats:sub>\u2010free), the pHi<\/jats:sub> was 7\u00b727, in bicarbonate\u2010buffered solution equilibrated with 6% CO2<\/jats:sub>, the mean pHi<\/jats:sub> was 7\u00b702.<\/jats:p>3. Application of 15 mM\u2010NH4<\/jats:sub>Cl produced a rapid intracellular alkalinization (0\u00b719 pH units), followed by a slower acidification. Removal of NH4<\/jats:sub>Cl gave rise to a slow and transient intracellular acidification (0\u00b75 pH units).<\/jats:p>4. The biphasic and transient shift in pHi<\/jats:sub>, induced by the NH4<\/jats:sub>Cl treatment, was accompanied by a change of the inside longitudinal resistance per unit fibre length, r<\/jats:italic>i<\/jats:sub>, displaying a similar time course. The increase in pHi<\/jats:sub> produced a maximum decrease in r<\/jats:italic>i<\/jats:sub> of 16\u00b74%, while the decrease in pHi<\/jats:sub> yielded a maximum increase in r<\/jats:italic>i<\/jats:sub> of 30\u00b74%.<\/jats:p>5. Changing from bicarbonate\u2010buffered Tyrode solution equilibrated with 6% CO2<\/jats:sub> to Tris\u2010buffered Tyrode solution led to an increase in pHi<\/jats:sub> (0\u00b726 pH units). A subsequent change to bicarbonate\u2010buffered Tyrode solution equilibrated with 15% CO2<\/jats:sub> produced a decrease in pHi<\/jats:sub> (0\u00b748 pH units). Both changes were sustained.<\/jats:p>6. This CO2<\/jats:sub> protocol gave rise to corresponding changes in r<\/jats:italic>i<\/jats:sub>; the intracellular alkalosis was associated with a decrease in r<\/jats:italic>i<\/jats:sub> (21\u00b72%), and the intracellular acidosis was accompanied by an increase in r<\/jats:italic>i<\/jats:sub> (30%).<\/jats:p>7. Based on recent findings showing an interaction between pHi<\/jats:sub> and pCai<\/jats:sub> (Hess & Weingart, 1980), it is concluded that the changes in r<\/jats:italic>i<\/jats:sub> are directly caused by protons and not indirectly via secondary changes of the [Ca2+<\/jats:sup>]i<\/jats:sub>.<\/jats:p>8. The pHi<\/jats:sub>\u2010dependent changes in r<\/jats:italic>i<\/jats:sub> are likely to reflect alterations of the nexal resistance, r<\/jats:italic>n<\/jats:sub>, because the cytoplasmic resistance, r<\/jats:italic>c<\/jats:sub>, has the inverse sensitivity to pHi<\/jats:sub>.<\/jats:p>9. Unlike pCai<\/jats:sub>, pHi<\/jats:sub> would seem to be able to modify the cell\u2010to\u2010cell coupling by increasing or decreasing r<\/jats:italic>i<\/jats:sub> over a rather narrow range, without ever producing electrical uncoupling.<\/jats:p>10. Because of basic differences in the action of calcium and protons on the cell\u2010to\u2010cell coupling (magnitude of the effect, operative concentration range), it is tempting to conclude that there is more than one kind of binding site which controls the nexal conductance.<\/jats:p>","DOI":"10.1113\/jphysiol.1982.sp014254","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:03:05Z","timestamp":1418972585000},"page":"87-104","source":"Crossref","is-referenced-by-count":72,"title":["Ungulate cardiac Purkinje fibres: the influence of intracellular pH on the electrical cell\u2010to\u2010cell coupling"],"prefix":"10.1113","volume":"328","author":[{"given":"Walter R.","family":"Reber","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Weingart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1982,7]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1982.sp014254","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1982.sp014254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,5]],"date-time":"2023-11-05T06:41:38Z","timestamp":1699166498000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1982.sp014254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1982,7]]}},"alternative-id":["10.1113\/jphysiol.1982.sp014254"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1982.sp014254","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,7]]}}}