{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:38:37Z","timestamp":1790642317403,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1982,12,1]],"date-time":"1982-12-01T00:00:00Z","timestamp":407548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1982,12]]},"abstract":"<jats:p>1. Catecholamine synthesis in rabbit carotid body was studied <jats:italic>in vitro<\/jats:italic> using [<jats:sup>3<\/jats:sup>H]DOPA and [<jats:sup>3<\/jats:sup>H]tyrosine as precursors. The effects of sympathectomy and transection of the carotid sinus nerve on [<jats:sup>3<\/jats:sup>H]dopamine ([<jats:sup>3<\/jats:sup>H]DA) and [<jats:sup>3<\/jats:sup>H]noradrenaline ([<jats:sup>3<\/jats:sup>H]NA) synthesis were investigated in chronically denervated carotid bodies.<\/jats:p><jats:p>2. When [<jats:sup>3<\/jats:sup>H]DOPA was used as precursor, the synthesis of [<jats:sup>3<\/jats:sup>H]DA was linear for more than 6 hr. The carotid body synthesized larger amounts of [<jats:sup>3<\/jats:sup>H]catecholamines than when [<jats:sup>3<\/jats:sup>H]tyrosine was used as precursor, but most of this excess was liberated into the incubation media. Using 10 \u03bcM\u2010[<jats:sup>3<\/jats:sup>H]DOPA as precursor, the synthesis rates were 6\u00b776 and 1\u00b751 n\u2010mole\/g per hr for [<jats:sup>3<\/jats:sup>H]DA and [<jats:sup>3<\/jats:sup>H]NA, respectively; with 40 \u03bcM\u2010[<jats:sup>3<\/jats:sup>H]DOPA, these values increased to 19\u00b722 and 3\u00b723 n\u2010mole\/g per hr, respectively.<\/jats:p><jats:p>3. The relationship between [<jats:sup>3<\/jats:sup>H]DOPA concentration and [<jats:sup>3<\/jats:sup>H]DA synthesis was linear throughout the range 5\u201040 \u03bcM\u2010[<jats:sup>3<\/jats:sup>H]DOPA.<\/jats:p><jats:p>4. Sympathectomy reduced the synthesis of [<jats:sup>3<\/jats:sup>H]NA by 90% and [<jats:sup>3<\/jats:sup>H]DA by 37% when [<jats:sup>3<\/jats:sup>H]DOPA was used as precursor.<\/jats:p><jats:p>5. When [<jats:sup>3<\/jats:sup>H]tyrosine (40 \u03bcM) was used as precursor, synthesis of [<jats:sup>3<\/jats:sup>H]catecholamines was linear for at least 4 hr, with rates of 12\u00b710 and 0\u00b785 n\u2010mole\/g per hr for [<jats:sup>3<\/jats:sup>H]DA and [<jats:sup>3<\/jats:sup>H]NA, respectively.<\/jats:p><jats:p>6. [<jats:sup>3<\/jats:sup>H]DA and [<jats:sup>3<\/jats:sup>H]NA synthesis from [<jats:sup>3<\/jats:sup>H]tyrosine exhibited the characteristics of saturable processes, with <jats:italic>K<jats:sub>m<\/jats:sub><\/jats:italic> values of 16\u00b78 and 17\u00b76 \u03bcM, respectively.<\/jats:p><jats:p>7. 6\u2010methyltetrahydropterine (6\u2010MPH<jats:sub>4<\/jats:sub>, 100 \u03bcM), a synthetic analogue of the natural co\u2010factor for tyrosine hydroxylase, increased [<jats:sup>3<\/jats:sup>H]DA and [<jats:sup>3<\/jats:sup>H]NA synthesis from [<jats:sup>3<\/jats:sup> H]tyrosine in both the carotid body and superior cervical ganglion, with the greatest effect seen in the carotid body.<\/jats:p><jats:p>8. When [<jats:sup>3<\/jats:sup>H]tyrosine was used as precursor, sympathectomy of the carotid body reduced [<jats:sup>3<\/jats:sup>H]NA synthesis by 80%, but did not alter [<jats:sup>3<\/jats:sup>H]DA or [<jats:sup>3<\/jats:sup>H]tyrosine levels in the tissue. Transection of the carotid sinus nerve had no effect on [<jats:sup>3<\/jats:sup>H]catecholamine synthesis in the carotid body.<\/jats:p>","DOI":"10.1113\/jphysiol.1982.sp014439","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:01:38Z","timestamp":1418972498000},"page":"69-79","source":"Crossref","is-referenced-by-count":71,"title":["Catecholamine synthesis in rabbit carotid body <i>in vitro<\/i>"],"prefix":"10.1113","volume":"333","author":[{"given":"S.","family":"Fidone","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1982,12]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1982.sp014439","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1982.sp014439","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T04:27:55Z","timestamp":1699244875000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1982.sp014439"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,12]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1982,12]]}},"alternative-id":["10.1113\/jphysiol.1982.sp014439"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1982.sp014439","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,12]]}}}