{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T23:06:34Z","timestamp":1771628794408,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1982,12,1]],"date-time":"1982-12-01T00:00:00Z","timestamp":407548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1982,12]]},"abstract":"<jats:p>1. Unanaesthetized, unrestrained rabbits were exposed for 3 hr in a chamber to either air, hypoxic gas mixtures (10% or 14% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub>) or a hyperoxic gas mixture (50% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub>). The carotid bodies were then removed and incubated for 3 hr in modified Tyrode media equilibrated with 100% O<jats:sub>2<\/jats:sub> and containing either [<jats:sup>3<\/jats:sup>H]tyrosine or [<jats:sup>3<\/jats:sup>H]DOPA. The contents of [<jats:sup>3<\/jats:sup>H]DA and [<jats:sup>3<\/jats:sup>H]NA in the tissue were determined as described in the preceding paper.<\/jats:p><jats:p>2. When [<jats:sup>3<\/jats:sup>H]DOPA was used as precursor, neither labelled dopamine (DA) or noradrenaline (NA) synthesis was increased in carotid bodies from rabbits exposed to 10% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub>. Following exposure to 10% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub> and incubation with [<jats:sup>3<\/jats:sup>H]tyrosine, however, [<jats:sup>3<\/jats:sup>H]DA synthesis was increased by 72% above control (air) values while [<jats:sup>3<\/jats:sup>H]NA synthesis was unchanged. Less severe hypoxia, 14% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub>, resulted in a smaller increase in [<jats:sup>3<\/jats:sup>H]DA synthesis, i.e. 53% above control value. Again, [<jats:sup>3<\/jats:sup>H]NA synthesis was unchanged. Similar experiments with the superior cervical ganglion involving exposure of the animals to either 10% or 14% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub> did not produce any change in the amounts of [<jats:sup>3<\/jats:sup>H]DA or [<jats:sup>3<\/jats:sup>H]NA synthesized from [<jats:sup>3<\/jats:sup>H]tyrosine when compared to control animals breathing air.<\/jats:p><jats:p>3. Sympathectomy of the carotid body or transection of the carotid sinus nerve 12\u201015 days prior to hypoxic exposure (10% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub>) did not alter the increase in [<jats:sup>3<\/jats:sup>H]DA synthesis compared to normally innervated carotid bodies.<\/jats:p><jats:p>4. Carotid bodies incubated with [<jats:sup>3<\/jats:sup>H]tyrosine for 2 hr in an alternating O<jats:sub>2<\/jats:sub>\/N<jats:sub>2<\/jats:sub> sequence (5 min in media equilibrated with 100% O<jats:sub>2<\/jats:sub> followed by 3 min in media equilibrated with 100% N<jats:sub>2<\/jats:sub>) synthesized 37% more [<jats:sup>3<\/jats:sup>H]DA than control carotid bodies similarly exposed to an alternating O<jats:sub>2<\/jats:sub>\/O<jats:sub>2<\/jats:sub> sequence. [<jats:sup>3<\/jats:sup>H]NA synthesis was unchanged. However, tissue levels of non\u2010metabolized [<jats:sup>3<\/jats:sup>H]tyrosine were reduced by 19% in the carotid bodies exposed to the O<jats:sub>2<\/jats:sub>\/N<jats:sub>2<\/jats:sub> sequence.<\/jats:p><jats:p>5. Exposure of rabbits for 3 hr to 50% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub>, followed by incubation of their carotid bodies in [<jats:sup>3<\/jats:sup>H]tyrosine, resulted in a 19% decrease in the absolute value for [<jats:sup>3<\/jats:sup>H]DA synthesis compared to control carotid bodies, but this difference was not significant (<jats:italic>P<\/jats:italic> &gt; 0\u00b705). However, [<jats:sup>3<\/jats:sup>H]NA synthesis was significantly reduced (51%; <jats:italic>P<\/jats:italic> &lt; 0\u00b705) in the hyperoxic carotid bodies. Similar experiments with the superior cervical ganglion showed that [<jats:sup>3<\/jats:sup>H]DA and [<jats:sup>3<\/jats:sup>H]NA synthesis were unchanged under control <jats:italic>vs.<\/jats:italic> hyperoxic conditions.<\/jats:p><jats:p>6. Carotid bodies incubated with [<jats:sup>3<\/jats:sup>H]tyrosine for 3 hr, then transferred for 1 hr to unlabelled media equilibrated with 10% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub>, released 81% more [<jats:sup>3<\/jats:sup>H]DA, and contained 38% less [<jats:sup>3<\/jats:sup>H]DA, than similarly treated carotid bodies exposed to 100% O<jats:sub>2<\/jats:sub>. [<jats:sup>3<\/jats:sup>H]NA was not detectable in the media, and tissue levels of [<jats:sup>3<\/jats:sup>H]NA were the same in both hypoxic and control carotid bodies.<\/jats:p>","DOI":"10.1113\/jphysiol.1982.sp014440","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:01:38Z","timestamp":1418972498000},"page":"81-91","source":"Crossref","is-referenced-by-count":49,"title":["Effects of hypoxia on catecholamine synthesis in rabbit carotid body <i>in vitro<\/i>"],"prefix":"10.1113","volume":"333","author":[{"given":"S.","family":"Fidone","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Yoshizaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1982,12]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1982.sp014440","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1982.sp014440","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T04:27:14Z","timestamp":1699244834000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1982.sp014440"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,12]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1982,12]]}},"alternative-id":["10.1113\/jphysiol.1982.sp014440"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1982.sp014440","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,12]]}}}