{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T04:30:47Z","timestamp":1778905847308,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1982,12,1]],"date-time":"1982-12-01T00:00:00Z","timestamp":407548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1982,12]]},"abstract":"<jats:p>1. Rabbit carotid bodies were pre\u2010loaded with [<jats:sup>3<\/jats:sup>H]dopamine (DA) synthesized from [<jats:sup>3<\/jats:sup>H]tyrosine and then mounted in a vertical drop\u2010type superfusion chamber which permitted simultaneous collection of released [<jats:sup>3<\/jats:sup>H]DA and recording of chemoreceptor discharge from the carotid sinus nerve.<\/jats:p><jats:p>2. The time course of the spontaneous release of [<jats:sup>3<\/jats:sup>H]DA (superfusion with media equilibrated with 100% O<jats:sub>2<\/jats:sub>) in the presence of monoamine oxidase inhibitors exhibited two linear components, an initial steep phase followed after 3\u20104 hr by a later slower phase of release.<\/jats:p><jats:p>3. When a 5 min low O<jats:sub>2<\/jats:sub> stimulus was delivered during the initial steep linear component of resting [<jats:sup>3<\/jats:sup>H]DA release, there was an abrupt increase in release, the magnitude of which was stimulus\u2010dependent.<\/jats:p><jats:p>4. The efflux of total radioactivity from the preparation declined exponentially with time; under resting conditions it was principally non\u2010metabolized [<jats:sup>3<\/jats:sup>H]tyrosine. During stimulation, however the efflux increased, and 60\u201080% of the radioactivity could be attributed to [<jats:sup>3<\/jats:sup>H]DA.<\/jats:p><jats:p>5. For a given low O<jats:sub>2<\/jats:sub> stimulus, the ratio of [<jats:sup>3<\/jats:sup>H]DA release during the stimulus period over that in the preceding control period remained approximately the same throughout a single experiment. Ratios for different low O<jats:sub>2<\/jats:sub> stimuli (50, 40, 30, 20, 10 and 0% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub>) yielded a parabolic relationship when plotted against stimulus intensity.<\/jats:p><jats:p>6. Transection of the carotid sinus nerve or removal of the superior cervical ganglion 12\u201015 days prior to the experiment did not affect the release of [<jats:sup>3<\/jats:sup>H]DA at moderate stimulus intensities (superfusion with media equilibrated with 30% or 10% O<jats:sub>2<\/jats:sub> in N<jats:sub>2<\/jats:sub>) but both procedures significantly depressed release at the highest stimulus intensity (100% N<jats:sub>2<\/jats:sub>).<\/jats:p><jats:p>7. Chemoreceptor discharge and [<jats:sup>3<\/jats:sup>H]DA release were simultaneously monitored in experiments using superfusion media free of monoamine oxidase inhibitors. In these experiments, the efflux of [<jats:sup>3<\/jats:sup>H]dihydroxyphenyl acetic acid (DOPAC) was also measured. The increase in peak chemosensory discharge was closely correlated with the increase in total release ([<jats:sup>3<\/jats:sup>H]DA + [<jats:sup>3<\/jats:sup>H]DOPAC) during stimulation with a series of low O<jats:sub>2<\/jats:sub> stimuli.<\/jats:p><jats:p>8. Release of [<jats:sup>3<\/jats:sup>H]DA was almost completely abolished during superfusion with Ca<jats:sup>2+<\/jats:sup>\u2010free, high Mg<jats:sup>2+<\/jats:sup> (2\u00b71 mM) media, and the stimulus\u2010related efflux of [<jats:sup>3<\/jats:sup>H]DOPAC was significantly reduced. However, chemoreceptor discharge was diminished by only 55%. These data are discussed with respect to their implications for DA as a chemosensory transmitter in rabbit carotid body.<\/jats:p>","DOI":"10.1113\/jphysiol.1982.sp014441","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T07:01:38Z","timestamp":1418972498000},"page":"93-110","source":"Crossref","is-referenced-by-count":206,"title":["Effects of low oxygen on the release of dopamine from the rabbit carotid body <i>in vitro<\/i>"],"prefix":"10.1113","volume":"333","author":[{"given":"S.","family":"Fidone","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Yoshizaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1982,12]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1982.sp014441","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1982.sp014441","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,6]],"date-time":"2023-11-06T04:27:47Z","timestamp":1699244867000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1982.sp014441"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,12]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1982,12]]}},"alternative-id":["10.1113\/jphysiol.1982.sp014441"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1982.sp014441","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,12]]}}}