{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T11:49:07Z","timestamp":1776426547313,"version":"3.51.2"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1984,7,1]],"date-time":"1984-07-01T00:00:00Z","timestamp":457488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1984,7]]},"abstract":"<jats:p>The patch\u2010clamp method was applied to single atrioventricular (a.v.) node cells of the rabbit heart to study the characteristics of the K+ channel. When the electrode contained 5.4 mM\u2010K+, depolarizations of the cell\u2010attached patch membrane induced outward single channel currents characterized by burst\u2010like openings; the open\u2010state probability increased from 0.005\u20100.01 at \u201040 mV to 0.07\u20100.1 at +20 mV of membrane potential. The reversal potentials of the current at K+ concentrations of 5.4, 20 and 130 mM in the electrode agreed with those given by the Nernst equation, indicating that this channel is selective for K+ ions. The slope conductance of the channel decreased beyond 60\u201090 mV positive to the reversal potential (inward\u2010going rectification). The conductance near the reversal potential increased with increasing K+ concentrations on either side of the membrane: from 31\u201032 pS at 5.4 mM\u2010K+ to 41\u201042 pS at 20 mM\u2010K+ on the outside, and from 19 pS at 90 mM\u2010K+ to 29.3 pS at 130 mM\u2010K+ on the inside. Superfusion of the cell with 5.4 mM\u2010CN\u2010, glucose\u2010free Tyrode markedly increased the number of channel openings in the cell\u2010attached patch. In the inside\u2010out patch, application of 1 mM\u2010adenosine\u20105'\u2010triphosphate (ATP) at the inner surface of the patch membrane blocked reversibly the channel activity, while 1 mM\u2010adenosine\u20105'\u2010diphosphate (ADP) failed to block it. The conductance and kinetics of the channel were not modified by increasing the Ca2+ concentration from 10(\u20108) M to 5 X 10(\u20106) M on the inner side of the membrane, while a further increase in Ca2+ to 10(\u20104) M decreased the open\u2010state probability. The probability density for the open time fitted well with an exponential distribution (time constant of 5.4 ms at 60 mV positive to the resting potential), while that for the closed time was separated into a fast and a slow component (time constants of 4.0 and 132.0 ms, respectively). The time constant of the slow component decreased significantly with depolarization in some preparations. However, neither the time constant of the fast component of the closed\u2010time histogram nor that of the open\u2010time histogram was voltage\u2010dependent.<\/jats:p>","DOI":"10.1113\/jphysiol.1984.sp015290","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T06:49:28Z","timestamp":1418971768000},"page":"265-284","source":"Crossref","is-referenced-by-count":93,"title":["Adenosine\u20105'\u2010triphosphate\u2010sensitive single potassium channel in the atrioventricular node cell of the rabbit heart."],"prefix":"10.1113","volume":"352","author":[{"given":"M","family":"Kakei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Noma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1984,7]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1984.sp015290","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1984.sp015290","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T03:07:06Z","timestamp":1697771226000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1984.sp015290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984,7]]}},"alternative-id":["10.1113\/jphysiol.1984.sp015290"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1984.sp015290","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,7]]}}}