{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T08:45:37Z","timestamp":1697877937081},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1984,7,1]],"date-time":"1984-07-01T00:00:00Z","timestamp":457488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1984,7]]},"abstract":"<jats:p>To characterize the role of cyclic nucleotides in secretion of enzymes by the lacrimal gland, pieces of rat exorbital glands were perfused with (1) 8\u2010bromoadenosine\u20103',5'\u2010cyclic monophosphate (8 Br cyclic AMP), (2) 8\u2010bromoguanosine\u20103',5'\u2010cyclic monophosphate (8 Br cyclic GMP), (3) forskolin, a stimulator of adenylate cyclase activity, (4) 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX), an inhibitor of phosphodiesterase activity, or (5) carbachol, a cholinergic agonist. As a measure of enzyme secretion, timed collections of the perifusate effluent were analysed for peroxidase, an enzyme secreted by the lacrimal gland. Control peroxidase secretion was 0.3\u20100.9 (u.\/min per milligram protein). Peroxidase secretion was stimulated by 8 Br cyclic AMP (1 mM), but not by 8 Br cyclic GMP (1 mM). A 2\u2010fold increase was detected. Peroxidase secretion was also stimulated by forskolin (60 microM), IBMX (1 mM), and the cholinergic agonist carbachol, which all stimulated peroxidase secretion 2\u2010or 3\u2010fold. The effect of maximally effective concentrations of IBMX (1 mM) and carbachol (0.1 mM) on secretion was additive. Finally, Ca2+ depletion in the presence of EGTA (1 mM) inhibited both IBMX\u2010and carbachol\u2010induced secretion by 45% and 60% respectively. We conclude that cyclic AMP, but not cyclic GMP, can stimulate lacrimal gland enzyme secretion. Cyclic AMP appears to utilize a pathway separate from but convergent with cholinergic agonists.<\/jats:p>","DOI":"10.1113\/jphysiol.1984.sp015297","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T06:49:28Z","timestamp":1418971768000},"page":"375-384","source":"Crossref","is-referenced-by-count":12,"title":["Cyclic nucleotide\u2010dependent enzyme secretion in the rat lacrimal gland."],"prefix":"10.1113","volume":"352","author":[{"given":"D A","family":"Dartt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Donowitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V J","family":"Joshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R S","family":"Mathieu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G W","family":"Sharp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1984,7]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1984.sp015297","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1984.sp015297","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T03:06:12Z","timestamp":1697771172000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1984.sp015297"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1984,7]]}},"alternative-id":["10.1113\/jphysiol.1984.sp015297"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1984.sp015297","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1984,7]]}}}