{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:50:32Z","timestamp":1758279032282},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1986,4,1]],"date-time":"1986-04-01T00:00:00Z","timestamp":512697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1986,4]]},"abstract":"<jats:p>Measurements of the intracellular activity of Ca (aiCa), Na (aiNa) and H (pHi) ions have been made with resin\u2010filled ion\u2010sensitive micro\u2010electrodes in ferret ventricular trabeculae. The mean values in quiescent muscle at 30 degrees C were: aiNa, 11.1 +\/\u2010 1.0 mM; aiCa, 58.4 +\/\u2010 6.4 nM, and pHi, 7.20 +\/\u2010 0.11. The relation between aiNa and extracellular Na activity (aoNa) is not linear and is sensitive to temperature: the Q10 for the change in aiNa in normal Tyrode solution is 1.3 +\/\u2010 0.5 and rises to 3.5 +\/\u2010 0.5 when aoNa is reduced to 1.1 mM. The addition of CN to the bathing fluid causes little or no change in aiNa or aiCa but pHi rises to 7.38 +\/\u2010 0.10, yet in some preparations resting tension increases. Similar results are seen with carbonyl cyanide m\u2010chlorophenyl hydrazone. On lowering [Na]o, the fall in aiNa is very much greater than the rise in aiCa and the pHi is generally unchanged. When [Na]o is lowered in the presence of a respiratory inhibitor, the fall in aiNa is reduced, the rise in aiCa and the contracture tension are increased while pHi falls. The apparent coupling ratio for the Na\/Ca exchange varies between 3 and 4 depending on the experimental conditions. These results suggest that an intracellular process, with a high Q10 and which depends upon respiration and aiNa, is able to remove Ca2+ from the sarcoplasm and thereby interact with the sarcolemmal Na\/Ca exchange. This process could be the increase in the energy\u2010dependent accumulation of Ca2+ within mitochondria that will occur when the Ca efflux from these organelles is progressively inhibited as aiNa falls.<\/jats:p>","DOI":"10.1113\/jphysiol.1986.sp016040","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T06:44:07Z","timestamp":1418971447000},"page":"163-179","source":"Crossref","is-referenced-by-count":48,"title":["Sodium\/calcium exchange and intracellular calcium buffering in ferret myocardium: an ion\u2010sensitive micro\u2010electrode study."],"prefix":"10.1113","volume":"373","author":[{"given":"R A","family":"Chapman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1986,4]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1986.sp016040","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1986.sp016040","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T22:42:15Z","timestamp":1697928135000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1986.sp016040"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,4]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1986,4]]}},"alternative-id":["10.1113\/jphysiol.1986.sp016040"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1986.sp016040","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,4]]}}}