{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:31:43Z","timestamp":1791185503586,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1986,11,1]],"date-time":"1986-11-01T00:00:00Z","timestamp":531187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1986,11]]},"abstract":"<jats:p>Experiments were conducted on anaesthetized, paralysed and artificially ventilated cats in order to determine if the altered discharge pattern of phrenic motoneurones recorded during stimulation of medullary raphe nuclei (raphe magnus (r. magnus), raphe obscurus (r. obscurus), raphe pallidus (r. pallidus] are related to release of serotonin (5\u2010hydroxytryptamine, 5\u2010HT) at synapses on respiratory neurones. Effects of 5\u2010HT released by the neurotoxin p\u2010chloroamphetamine (PCA) on spontaneous activity of phrenic motoneurones were also examined. In addition, responses of phrenic motoneurones to 5\u2010HT applied by micro\u2010electrophoresis were recorded. Stimulation (100 Hz) of r. magnus or r. obscurus depressed the spontaneous inspiratory discharges of phrenic motoneurones. Administration of the 5\u2010HT receptor antagonists cinanserin, methysergide or methergoline reduced, but did not abolish, the inhibition. Inhibition of the neuronal reuptake of 5\u2010HT with fluoxetine enhanced the inhibition and reduced the peak inspiratory action potential frequency of spontaneous discharges. Stimulation (100 Hz) of r. pallidus produced increased firing of phrenic motoneurones. Firing of phrenic motoneurones was evoked even during the normally quiescent expiratory phase of spontaneous respiratory activity. Antagonists at 5\u2010HT receptors reduced the degree of tonic firing, resulting in partial restoration of expiratory pauses during r. pallidus stimulation. Inhibition of 5\u2010HT reuptake, on the other hand, resulted in increased tonic firing. Release of 5\u2010HT by PCA produced a rapid and severe reduction of phrenic nerve activity. Activity was restored by 5\u2010HT receptor antagonists. In reserpine\u2010treated cats, effects of stimulating medullary raphe nuclei were still pronounced, however 5\u2010HT receptor antagonists had no effect on the responses. These results, along with the observation that 5\u2010HT receptor antagonists are only partially effective in non\u2010reserpinized cats, indicate that non\u2010serotoninergic influences contribute as well to the responses evoked by raphe stimulation. Micro\u2010electrophoretic application of 5\u2010HT by large ejecting currents (100\u2010200 nA) had weak stimulatory effects on twenty of forty\u2010five phrenic motoneurones, which exhibited small increases in peak inspiratory discharge frequency during 5\u2010HT application. Ejecting currents less than 100 nA were without effect. It is concluded that 5\u2010HT analogues and agents which release endogenous 5\u2010HT after parenteral administration do not act directly at synapses on the soma membranes of phrenic motoneurones.(ABSTRACT TRUNCATED AT 400 WORDS)<\/jats:p>","DOI":"10.1113\/jphysiol.1986.sp016291","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T06:42:56Z","timestamp":1418971376000},"page":"373-385","source":"Crossref","is-referenced-by-count":101,"title":["Serotoninergic and non\u2010serotoninergic responses of phrenic motoneurones to raphe stimulation in the cat."],"prefix":"10.1113","volume":"380","author":[{"given":"P M","family":"Lalley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1986,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1986.sp016291","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1986.sp016291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T17:08:44Z","timestamp":1697821724000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1986.sp016291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,11]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1986,11]]}},"alternative-id":["10.1113\/jphysiol.1986.sp016291"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1986.sp016291","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,11]]}}}