{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T22:45:24Z","timestamp":1775947524399,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1987,2,1]],"date-time":"1987-02-01T00:00:00Z","timestamp":539136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1987,2]]},"abstract":"<jats:p>1. Ca\u2010selective micro\u2010electrodes were used to measure free Ca concentration in axons and extruded axoplasm. 2. Free Ca in axons immersed in artificial sea water containing 3 mM\u2010Ca averaged 77 nM in freshly dissected axons and 4.9 microM in cyanide\u2010 or azide\u2010poisoned axons. 3. Extruded axoplasm maintained a free Ca only a little higher than that of the axons from which it was obtained. 4. Axoplasmic buffering was investigated by titrating isolated axoplasm with CaCl2 or K\u2010EGTA and monitoring the change in free Ca. Energy\u2010dependent and energy\u2010independent components of Ca binding could be recognized in fresh axoplasm. The energy\u2010dependent fraction could be further subdivided into Ruthenium Red\u2010sensitive and Ruthenium Red\u2010insensitive components and the energy\u2010independent fraction into a component of high affinity and rather low capacity and another component of low affinity and large capacity. 5. The Ruthenium Red\u2010sensitive process could accumulate many millimoles Ca per kilogram axoplasm while still maintaining a free Ca close to 100 nM. After injection of Ruthenium Red into fresh axoplasm, binding is dramatically altered so that it closely resembles that in a metabolically poisoned preparation. 6. The Ruthenium Red\u2010insensitive process has a small capacity and appears to be capable of lowering free Ca to about 200 nM. It can, however, lower free Ca to 50\u2010150 nM if oxalate is also present. 7. Simultaneous measurement of pH and free Ca showed that axoplasmic pH only begins to fall appreciably in response to added Ca when mitochondrial Ca buffering becomes impaired. 8. Raising axoplasmic levels of Na or Li, but not K, tends to bring about a concomitant rise in free Ca.<\/jats:p>","DOI":"10.1113\/jphysiol.1987.sp016414","type":"journal-article","created":{"date-parts":[[2015,12,16]],"date-time":"2015-12-16T14:13:17Z","timestamp":1450275197000},"page":"369-394","source":"Crossref","is-referenced-by-count":34,"title":["Calcium buffering in axons and axoplasm of Loligo."],"prefix":"10.1113","volume":"383","author":[{"given":"P F","family":"Baker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Umbach","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1987,2]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1987.sp016414","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1987.sp016414","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1987.sp016414","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T05:54:49Z","timestamp":1697867689000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1987.sp016414"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1987,2]]}},"alternative-id":["10.1113\/jphysiol.1987.sp016414"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1987.sp016414","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,2]]}}}