{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T00:33:29Z","timestamp":1790987609215,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1987,5,1]],"date-time":"1987-05-01T00:00:00Z","timestamp":546825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1987,5]]},"abstract":"<jats:p>1. Intracellular recordings from cultured rat spinal cord neurones demonstrated that Cl(\u2010)\u2010dependent responses to GABA (gamma\u2010aminobutyric acid) (but not glycine) were increased in amplitude and duration by the steroid anaesthetic alphaxalone (3 alpha\u2010hydroxy\u20105 alpha\u2010pregnane\u201011,20\u2010dione) at submicromolar concentrations that produced little or no effect on passive electrical properties. The non\u2010anaesthetic 3 beta\u2010hydroxy analogue was without effect on GABA\u2010evoked responses. 2. Under voltage clamp, membrane currents evoked by GABA were potentiated by alphaxalone without change in the reversal potential for the GABA\u2010evoked response. Fluctuation analysis of GABA\u2010evoked currents suggested that the mean open\u2010time of GABA\u2010activated channels was prolonged from 30 to 74 ms in the presence of the anaesthetic. 3. Higher concentrations of alphaxalone, similar to those reported during surgical anaesthesia, increased membrane conductance in the absence of exogenously applied GABA. Under voltage clamp, current responses to alphaxalone reversed at the same potential as did responses to GABA, suggesting that they result from increased Cl\u2010 conductance. 4. Alphaxalone responses were reduced by the GABA antagonist bicuculline. Fluctuation analysis of current responses to the anaesthetic suggest that they result from the activation of ion channels of long (100 ms) open\u2010time and elementary conductance indistinguishable from that of channels activated by GABA (20 pS). Taken together, these findings indicate that the steroid anaesthetic is able to directly activate Cl\u2010 conductance normally activated by GABA in spinal neurones. 5. The actions of the steroid at GABA\u2010receptor\u2010Cl(\u2010)\u2010channel complexes are similar to those produced by the anaesthetic barbiturates (e.g. pentobarbitone), although obtained at 50\u2010100\u2010fold lower concentrations. These effects on the inhibitory Cl(\u2010)\u2010conductance mechanism may be partly responsible for the depressant actions of alphaxalone on the mammalian central nervous system.<\/jats:p>","DOI":"10.1113\/jphysiol.1987.sp016547","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T06:41:29Z","timestamp":1418971289000},"page":"485-501","source":"Crossref","is-referenced-by-count":141,"title":["Potentiation of gamma\u2010aminobutyric\u2010acid\u2010activated chloride conductance by a steroid anaesthetic in cultured rat spinal neurones."],"prefix":"10.1113","volume":"386","author":[{"given":"J L","family":"Barker","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N L","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G D","family":"Lange","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D G","family":"Owen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1987,5]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1987.sp016547","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1987.sp016547","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T15:21:21Z","timestamp":1697901681000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1987.sp016547"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,5]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1987,5]]}},"alternative-id":["10.1113\/jphysiol.1987.sp016547"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1987.sp016547","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,5]]}}}