{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T22:35:17Z","timestamp":1768084517797,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1988,9,1]],"date-time":"1988-09-01T00:00:00Z","timestamp":589075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1988,9]]},"abstract":"<jats:p>1. The stimulatory action of propionate on colonic electrolyte transport and involvement of the enteric reflex in this was studied in vitro using an Ussing chamber in the rat. The short\u2010circuit current (Isc) and bidirectional fluxes of Na+ and Cl\u2010 were measured. Mucosa\u2010submucosa preparations, containing the submucosal nerve, from the distal colon were used in most cases. 2. Mucosal application of propionate caused transient increases in the transmural potential difference, with the mucosal side negative, Isc and conductance. Serosal application of the acid had no effect. 3. Adaptation of the Isc response occurred when the acid was applied to the bathing solution cumulatively without washing out the first dose. If tissues were washed and held more than 20 min before the next application, the response was almost completely restored. 4. The increase in Isc in response to propionate was concentration dependent, with a 50% effective concentration of approximately 7 x 10(\u20105) M. 5. Two other short\u2010chain fatty acids (SCFAs), n\u2010butyrate and n\u2010valerate, but not acetate, increased Isc when added to the mucosal bathing solution. 6. Bumetanide (3 x 10(\u20105) M) and the serosal chloride\u2010free condition, but not amiloride (10(\u20104) M), inhibited the responses of Isc to propionate. Propionate\u2010stimulated Cl\u2010 secretion resulted mainly from an increase in unidirectional serosal\u2010to\u2010mucosal Cl\u2010 movement. Propionate did not affect the Na+ flux. 7. Tetrodotoxin (10(\u20107) M), somatostatin (10(\u20107) M) and hexamethonium (10(\u20104) M) inhibited the propionate\u2010evoked increase in Isc by 40, 70 and 30%, respectively. 8. Atropine (10(\u20105) M) also inhibited the Isc\u2010increase response to propionate more than 90%. 9. Pre\u2010treatment (2 min) of the mucosal surface with procaine (5 x 10(\u20104) M) inhibited the propionate\u2010evoked increase in Isc by 90%. 10. The results suggest that luminal propionate transiently stimulated the colonic chloride secretory response that is not due to direct action on colonocytes, but due in large part to release of acetylcholine at neuro\u2010colonocyte junctions, probably via an enteric reflex involving a mucosal sensory mechanism, cholinergic motor nerves and submucosal ganglia.<\/jats:p>","DOI":"10.1113\/jphysiol.1988.sp017264","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T06:27:02Z","timestamp":1418970422000},"page":"559-575","source":"Crossref","is-referenced-by-count":54,"title":["Luminal propionate\u2010induced secretory response in the rat distal colon in vitro."],"prefix":"10.1113","volume":"403","author":[{"given":"T","family":"Yajima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1988,9]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1988.sp017264","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1988.sp017264","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T12:08:42Z","timestamp":1697976522000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1988.sp017264"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,9]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,9]]}},"alternative-id":["10.1113\/jphysiol.1988.sp017264"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1988.sp017264","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,9]]}}}